Murine aggregation chimeras and wholemount imaging in airway stem cell biology

Ian R Rosewell; Adam Giangreco

doi:10.1007/978-1-61779-980-8_20

Murine aggregation chimeras and wholemount imaging in airway stem cell biology

Ian R Rosewell, Adam Giangreco^*

^*Corresponding author for this work

Research output: Contribution to journal › Report/ Case Report › peer-review

1 Citation (Scopus)

Abstract

Local tissue stem cells are known to exist in mammalian lungs but their role in epithelial maintenance remains unclear. We therefore developed murine aggregation chimera and wholemount imaging techniques to assess the contribution of these cells to lung homeostasis and repair. In this chapter we provide further details regarding the generation of murine aggregation chimera mice and their subsequent use in wholemount lung imaging. We also describe methods related to the interpretation of this data that allows for quantitative assessment of airway stem cell activation versus quiescence. Using these techniques, it is possible to compare the growth and differentiation capacity of various lung epithelial cells in normal, repairing, and diseased states.

Original language	English
Pages (from-to)	263-74
Number of pages	12
Journal	Methods in Molecular Biology
Volume	916
DOIs	https://doi.org/10.1007/978-1-61779-980-8_20
Publication status	Print publication - 2012
Externally published	Yes

Keywords

Animals
Embryo Implantation
Embryo, Mammalian/embryology
Female
Fertilization
Hybridization, Genetic
Image Processing, Computer-Assisted
Lung/cytology
Male
Mice
Microdissection
Microscopy, Confocal
Molecular Imaging/methods
Pregnancy
Stem Cells/cytology

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10.1007/978-1-61779-980-8_20

Cite this

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title = "Murine aggregation chimeras and wholemount imaging in airway stem cell biology",

abstract = "Local tissue stem cells are known to exist in mammalian lungs but their role in epithelial maintenance remains unclear. We therefore developed murine aggregation chimera and wholemount imaging techniques to assess the contribution of these cells to lung homeostasis and repair. In this chapter we provide further details regarding the generation of murine aggregation chimera mice and their subsequent use in wholemount lung imaging. We also describe methods related to the interpretation of this data that allows for quantitative assessment of airway stem cell activation versus quiescence. Using these techniques, it is possible to compare the growth and differentiation capacity of various lung epithelial cells in normal, repairing, and diseased states.",

keywords = "Animals, Embryo Implantation, Embryo, Mammalian/embryology, Female, Fertilization, Hybridization, Genetic, Image Processing, Computer-Assisted, Lung/cytology, Male, Mice, Microdissection, Microscopy, Confocal, Molecular Imaging/methods, Pregnancy, Stem Cells/cytology",

author = "Rosewell, {Ian R} and Adam Giangreco",

year = "2012",

doi = "10.1007/978-1-61779-980-8_20",

language = "English",

volume = "916",

pages = "263--74",

journal = "Methods in Molecular Biology",

issn = "1064-3745",

publisher = "Humana Press",

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TY - JOUR

T1 - Murine aggregation chimeras and wholemount imaging in airway stem cell biology

AU - Rosewell, Ian R

AU - Giangreco, Adam

PY - 2012

Y1 - 2012

N2 - Local tissue stem cells are known to exist in mammalian lungs but their role in epithelial maintenance remains unclear. We therefore developed murine aggregation chimera and wholemount imaging techniques to assess the contribution of these cells to lung homeostasis and repair. In this chapter we provide further details regarding the generation of murine aggregation chimera mice and their subsequent use in wholemount lung imaging. We also describe methods related to the interpretation of this data that allows for quantitative assessment of airway stem cell activation versus quiescence. Using these techniques, it is possible to compare the growth and differentiation capacity of various lung epithelial cells in normal, repairing, and diseased states.

AB - Local tissue stem cells are known to exist in mammalian lungs but their role in epithelial maintenance remains unclear. We therefore developed murine aggregation chimera and wholemount imaging techniques to assess the contribution of these cells to lung homeostasis and repair. In this chapter we provide further details regarding the generation of murine aggregation chimera mice and their subsequent use in wholemount lung imaging. We also describe methods related to the interpretation of this data that allows for quantitative assessment of airway stem cell activation versus quiescence. Using these techniques, it is possible to compare the growth and differentiation capacity of various lung epithelial cells in normal, repairing, and diseased states.

KW - Animals

KW - Embryo Implantation

KW - Embryo, Mammalian/embryology

KW - Female

KW - Fertilization

KW - Hybridization, Genetic

KW - Image Processing, Computer-Assisted

KW - Lung/cytology

KW - Male

KW - Mice

KW - Microdissection

KW - Microscopy, Confocal

KW - Molecular Imaging/methods

KW - Pregnancy

KW - Stem Cells/cytology

U2 - 10.1007/978-1-61779-980-8_20

DO - 10.1007/978-1-61779-980-8_20

M3 - Report/ Case Report

C2 - 22914947

SN - 1064-3745

VL - 916

SP - 263

EP - 274

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Murine aggregation chimeras and wholemount imaging in airway stem cell biology

Abstract

Keywords

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