Necl2 regulates epidermal adhesion and wound repair

Adam Giangreco, Kim B Jensen, Yoshimi Takai, Jun Miyoshi, Fiona M Watt

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)


Differential expression of cell adhesion molecules regulates stem cell location, self-renewal and lineage selection under steady state conditions and during tissue repair. We show that the intercellular adhesion protein nectin-like molecule 2 (Necl2) is highly expressed in bulge stem cells of adult human and mouse hair follicles. Overexpression of Necl2 in cultured human keratinocytes led to upregulation of calcium/calmodulin-associated Ser/Thr kinase (CASK), increased calcium-independent intercellular adhesion, and inhibition of cell motility and in vitro wound healing. Although the rate of cell proliferation was reduced, terminal differentiation was unaffected. To assess the role of Necl2 in vivo, we examined the epidermis of Necl2-null mice and developed transgenic mice that expressed Necl2 in the basal layer of murine epidermis. Necl2 overexpression led to a reduction in S-phase cells and an increase in quiescent cells retaining DNA label in the bulge. Although epidermal homeostasis appeared normal in both transgenic and knockout mice, wound healing was markedly delayed. Necl2 overexpression resulted in reduced proliferation and increased levels of CASK and E-cadherin at the leading edge of healing wounds, consistent with its effects in culture. Our results demonstrate that Necl2 is involved in regulating epidermal stem cell quiescence and location.

Original languageEnglish
Pages (from-to)3505-14
Number of pages10
JournalDevelopment (Cambridge)
Issue number20
Publication statusPrint publication - Oct 2009
Externally publishedYes


  • Animals
  • Cell Adhesion
  • Cell Adhesion Molecule-1
  • Cell Adhesion Molecules
  • Cell Differentiation
  • Cell Proliferation
  • Cells, Cultured
  • Epidermal Cells
  • Epidermis/metabolism
  • Homeostasis
  • Humans
  • Immunoglobulins/deficiency
  • Keratinocytes/cytology
  • Membrane Proteins/deficiency
  • Mice
  • Mice, Knockout
  • Stem Cells/cytology
  • Tumor Suppressor Proteins/metabolism
  • Up-Regulation
  • Wound Healing


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