New Matrixes to Diagnose Aethina tumida Presence at Apiary Level

Antonella Cersini, Marco Pietropaoli*, Gabriele Pietrella, Jorge Rivera-Gomis, Giovanni Federico, Silvia Tofani, Raffaella Conti, Roberta C. Rubino, Camilla Di Ruggiero, Giovanni Formato

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Aethina tumida (Coleoptera: Nitidulidae - Small Hive Beetle - SHB), is a parasite of honey bee colonies that causes the notifiable disease called aethinosis. In 2014, SHB was detected in Southern Italy, where it is still present (Calabria region). As part of surveillance activities, official diagnosis of the disease is performed by veterinary services via visual inspection of single hives in the apiaries. New outbreaks can be eradicated and the spread of SHBs limited by early detection of new introductions. We report an alternative protocol for the diagnosis of SHB through swabs and hive debris analysis by PCR. This was tested in three apiaries through the evaluation of different SHB infestation levels with a hive inspection method. This approach for sampling, followed by biomolecular methods, was fast and useful in unfavorable conditions (bad weather, aggressiveness, robbing), could be integrated in the official diagnosis procedures and may act as pre-clinic indicator.

Original languageEnglish
Pages (from-to)345-348
Number of pages4
JournalJournal of Apicultural Science
Issue number2
Publication statusPrint publication - 1 Dec 2021
Externally publishedYes


  • Aethina tumida
  • hive debris
  • PCR
  • swab


Dive into the research topics of 'New Matrixes to Diagnose Aethina tumida Presence at Apiary Level'. Together they form a unique fingerprint.

Cite this