Novel Escherichia coli-Infecting Bacteriophages Isolated from Uganda That Target Human Clinical Isolates

Janet Yakubu Nale, Benjamin Chan, Nnaemeka Emmanuel Nnadi, Jeffrey Kwok Jone Cheng, Susan Matts, Neda Nezam-Abadi, Christopher Jason Richard Turkington, Lucie Manon Charreton, Harroop Bola, Ramez Nazir, Abubakar Shaaban Hoza, Samuel Posian Wamala, Ivan Ibanda, Alice Nyambura Maina, Auleria Ajiambo Apopo, Venance Theophil Msoffe, Nyambura Moremi, Grace Wanjiru Moore, Ismail Asiimwe, Alice NamatovuPaul Mutumba, Deus Kamya, Ritah Nabunje, Immaculate Nakabugo, Rudovick Ruben Kazwala, Erastus Kangethe, Abel Abera Negash, Arthur Kalyebi Watelo, Nelson Bukamba, Gideon Muhindo, Nathan Musisi Lubowa, Ngalla Jillani, Atunga Nyachieo, George Nasinyama, Jesca Nakavuma, Andrew Millard, Tobi Elaine Nagel, Martha Rebecca Jane Clokie*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

1 Citation (Scopus)
45 Downloads (Pure)

Abstract

Background: The antimicrobial resistance catastrophe is a growing global health threat and predicted to be worse in developing countries. Phages for Global Health (PGH) is training scientists in these regions to isolate relevant therapeutic phages for pathogenic bacteria within their locality, and thus contributing to making phage technology universally available.

Materials and Methods: During the inaugural PGH workshop in East Africa, samples from Ugandan municipal sewage facilities were collected and two novel Escherichia coli lytic phages were isolated and characterized.

Results: The phages, UP19 (capsid diameter ∼100 nm, contractile tail ∼120/20 nm) and UP30 (capsid diameter ∼70 nm, noncontractile tail of ∼170/20 nm), lysed ∼82% and ∼36% of the 11 clinical isolates examined, respectively. The genomes of UP19 (171.402 kb, 282 CDS) and UP30 (49.834 kb, 75 CDS) closely match the genera Dhakavirus and Tunavirus, respectively.

Conclusion: The phages isolated have therapeutic potential for further development against E. coli infections.
Original languageEnglish
Pages (from-to)141-149
Number of pages9
JournalPHAGE: Therapy, Applications, and Research
Volume4
Issue number3
Early online date20 Sept 2023
DOIs
Publication statusFirst published - 20 Sept 2023

Bibliographical note

Copyright 2023, Mary Ann Liebert, Inc., publishers.

Keywords

  • Phages for global health
  • phage therapy
  • Escherichia coli phage
  • antimicrobial resistance
  • Phages for Global Health

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