Novel Escherichia coli-Infecting Bacteriophages Isolated from Uganda That Target Human Clinical Isolates

Janet Yakubu Nale; Benjamin Chan; Nnaemeka Emmanuel Nnadi; Jeffrey Kwok Jone Cheng; Susan Matts; Neda Nezam-Abadi; Christopher Jason Richard Turkington; Lucie Manon Charreton; Harroop Bola; Ramez Nazir; Abubakar Shaaban Hoza; Samuel Posian Wamala; Ivan Ibanda; Alice Nyambura Maina; Auleria Ajiambo Apopo; Venance Theophil Msoffe; Nyambura Moremi; Grace Wanjiru Moore; Ismail Asiimwe; Alice Namatovu; Paul Mutumba; Deus Kamya; Ritah Nabunje; Immaculate Nakabugo; Rudovick Ruben Kazwala; Erastus Kangethe; Abel Abera Negash; Arthur Kalyebi Watelo; Nelson Bukamba; Gideon Muhindo; Nathan Musisi Lubowa; Ngalla Jillani; Atunga Nyachieo; George Nasinyama; Jesca Nakavuma; Andrew Millard; Tobi Elaine Nagel; Martha Rebecca Jane Clokie

doi:10.1089/phage.2023.0012

Novel Escherichia coli-Infecting Bacteriophages Isolated from Uganda That Target Human Clinical Isolates

Janet Yakubu Nale
, Benjamin Chan
, Nnaemeka Emmanuel Nnadi
, Jeffrey Kwok Jone Cheng
, Susan Matts
, Neda Nezam-Abadi
, Christopher Jason Richard Turkington
, Lucie Manon Charreton
, Harroop Bola
, Ramez Nazir
, Abubakar Shaaban Hoza
, Samuel Posian Wamala
, Ivan Ibanda
, Alice Nyambura Maina
, Auleria Ajiambo Apopo
, Venance Theophil Msoffe
, Nyambura Moremi
, Grace Wanjiru Moore
, Ismail Asiimwe
, Alice Namatovu

Paul Mutumba, Deus Kamya, Ritah Nabunje, Immaculate Nakabugo, Rudovick Ruben Kazwala, Erastus Kangethe, Abel Abera Negash, Arthur Kalyebi Watelo, Nelson Bukamba, Gideon Muhindo, Nathan Musisi Lubowa, Ngalla Jillani, Atunga Nyachieo, George Nasinyama, Jesca Nakavuma, Andrew Millard, Tobi Elaine Nagel, Martha Rebecca Jane Clokie^*

^*Corresponding author for this work

Centre for Epidemiology and Planetary Health (CEPH)

Research output: Contribution to journal › Review article › peer-review

6 Citations (Scopus)

126 Downloads (Pure)

Abstract

Background: The antimicrobial resistance catastrophe is a growing global health threat and predicted to be worse in developing countries. Phages for Global Health (PGH) is training scientists in these regions to isolate relevant therapeutic phages for pathogenic bacteria within their locality, and thus contributing to making phage technology universally available.

Materials and Methods: During the inaugural PGH workshop in East Africa, samples from Ugandan municipal sewage facilities were collected and two novel Escherichia coli lytic phages were isolated and characterized.

Results: The phages, UP19 (capsid diameter ∼100 nm, contractile tail ∼120/20 nm) and UP30 (capsid diameter ∼70 nm, noncontractile tail of ∼170/20 nm), lysed ∼82% and ∼36% of the 11 clinical isolates examined, respectively. The genomes of UP19 (171.402 kb, 282 CDS) and UP30 (49.834 kb, 75 CDS) closely match the genera Dhakavirus and Tunavirus, respectively.

Conclusion: The phages isolated have therapeutic potential for further development against E. coli infections.

Original language	English
Pages (from-to)	141-149
Number of pages	9
Journal	PHAGE: Therapy, Applications, and Research
Volume	4
Issue number	3
Early online date	20 Sept 2023
DOIs	https://doi.org/10.1089/phage.2023.0012
Publication status	First published - 20 Sept 2023

Bibliographical note

Keywords

Phages for global health
phage therapy
Escherichia coli phage
antimicrobial resistance
Phages for Global Health

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10.1089/phage.2023.0012

PHAGE-2023-0012-Nale_1P_jynAccepted author manuscript, 2.51 MBLicence: CC BY

Cite this

Nale, J. Y., Chan, B., Nnadi, N. E., Cheng, J. K. J., Matts, S., Nezam-Abadi, N., Turkington, C. J. R., Charreton, L. M., Bola, H., Nazir, R., Hoza, A. S., Wamala, S. P., Ibanda, I., Maina, A. N., Apopo, A. A., Msoffe, V. T., Moremi, N., Moore, G. W., Asiimwe, I., ... Clokie, M. R. J. (2023). Novel Escherichia coli-Infecting Bacteriophages Isolated from Uganda That Target Human Clinical Isolates. PHAGE: Therapy, Applications, and Research, 4(3), 141-149. Advance online publication. https://doi.org/10.1089/phage.2023.0012

@article{887e5c3323724b2aa85b2cf625769634,

title = "Novel Escherichia coli-Infecting Bacteriophages Isolated from Uganda That Target Human Clinical Isolates",

abstract = "Background: The antimicrobial resistance catastrophe is a growing global health threat and predicted to be worse in developing countries. Phages for Global Health (PGH) is training scientists in these regions to isolate relevant therapeutic phages for pathogenic bacteria within their locality, and thus contributing to making phage technology universally available. Materials and Methods: During the inaugural PGH workshop in East Africa, samples from Ugandan municipal sewage facilities were collected and two novel Escherichia coli lytic phages were isolated and characterized. Results: The phages, UP19 (capsid diameter ∼100 nm, contractile tail ∼120/20 nm) and UP30 (capsid diameter ∼70 nm, noncontractile tail of ∼170/20 nm), lysed ∼82\% and ∼36\% of the 11 clinical isolates examined, respectively. The genomes of UP19 (171.402 kb, 282 CDS) and UP30 (49.834 kb, 75 CDS) closely match the genera Dhakavirus and Tunavirus, respectively. Conclusion: The phages isolated have therapeutic potential for further development against E. coli infections.",

keywords = "Phages for global health, phage therapy, Escherichia coli phage, antimicrobial resistance, Phages for Global Health",

author = "Nale, \{Janet Yakubu\} and Benjamin Chan and Nnadi, \{Nnaemeka Emmanuel\} and Cheng, \{Jeffrey Kwok Jone\} and Susan Matts and Neda Nezam-Abadi and Turkington, \{Christopher Jason Richard\} and Charreton, \{Lucie Manon\} and Harroop Bola and Ramez Nazir and Hoza, \{Abubakar Shaaban\} and Wamala, \{Samuel Posian\} and Ivan Ibanda and Maina, \{Alice Nyambura\} and Apopo, \{Auleria Ajiambo\} and Msoffe, \{Venance Theophil\} and Nyambura Moremi and Moore, \{Grace Wanjiru\} and Ismail Asiimwe and Alice Namatovu and Paul Mutumba and Deus Kamya and Ritah Nabunje and Immaculate Nakabugo and Kazwala, \{Rudovick Ruben\} and Erastus Kangethe and Negash, \{Abel Abera\} and Watelo, \{Arthur Kalyebi\} and Nelson Bukamba and Gideon Muhindo and Lubowa, \{Nathan Musisi\} and Ngalla Jillani and Atunga Nyachieo and George Nasinyama and Jesca Nakavuma and Andrew Millard and Nagel, \{Tobi Elaine\} and Clokie, \{Martha Rebecca Jane\}",

year = "2023",

month = sep,

day = "20",

doi = "10.1089/phage.2023.0012",

language = "English",

volume = "4",

pages = "141--149",

journal = "PHAGE: Therapy, Applications, and Research",

issn = "2641-6530",

publisher = "SAGE Publications Ltd",

number = "3",

}

Nale, JY, Chan, B, Nnadi, NE, Cheng, JKJ, Matts, S, Nezam-Abadi, N, Turkington, CJR, Charreton, LM, Bola, H, Nazir, R, Hoza, AS, Wamala, SP, Ibanda, I, Maina, AN, Apopo, AA, Msoffe, VT, Moremi, N, Moore, GW, Asiimwe, I, Namatovu, A, Mutumba, P, Kamya, D, Nabunje, R, Nakabugo, I, Kazwala, RR, Kangethe, E, Negash, AA, Watelo, AK, Bukamba, N, Muhindo, G, Lubowa, NM, Jillani, N, Nyachieo, A, Nasinyama, G, Nakavuma, J, Millard, A, Nagel, TE & Clokie, MRJ 2023, 'Novel Escherichia coli-Infecting Bacteriophages Isolated from Uganda That Target Human Clinical Isolates', PHAGE: Therapy, Applications, and Research, vol. 4, no. 3, pp. 141-149. https://doi.org/10.1089/phage.2023.0012

TY - JOUR

T1 - Novel Escherichia coli-Infecting Bacteriophages Isolated from Uganda That Target Human Clinical Isolates

AU - Nale, Janet Yakubu

AU - Chan, Benjamin

AU - Nnadi, Nnaemeka Emmanuel

AU - Cheng, Jeffrey Kwok Jone

AU - Matts, Susan

AU - Nezam-Abadi, Neda

AU - Turkington, Christopher Jason Richard

AU - Charreton, Lucie Manon

AU - Bola, Harroop

AU - Nazir, Ramez

AU - Hoza, Abubakar Shaaban

AU - Wamala, Samuel Posian

AU - Ibanda, Ivan

AU - Maina, Alice Nyambura

AU - Apopo, Auleria Ajiambo

AU - Msoffe, Venance Theophil

AU - Moremi, Nyambura

AU - Moore, Grace Wanjiru

AU - Asiimwe, Ismail

AU - Namatovu, Alice

AU - Mutumba, Paul

AU - Kamya, Deus

AU - Nabunje, Ritah

AU - Nakabugo, Immaculate

AU - Kazwala, Rudovick Ruben

AU - Kangethe, Erastus

AU - Negash, Abel Abera

AU - Watelo, Arthur Kalyebi

AU - Bukamba, Nelson

AU - Muhindo, Gideon

AU - Lubowa, Nathan Musisi

AU - Jillani, Ngalla

AU - Nyachieo, Atunga

AU - Nasinyama, George

AU - Nakavuma, Jesca

AU - Millard, Andrew

AU - Nagel, Tobi Elaine

AU - Clokie, Martha Rebecca Jane

PY - 2023/9/20

Y1 - 2023/9/20

N2 - Background: The antimicrobial resistance catastrophe is a growing global health threat and predicted to be worse in developing countries. Phages for Global Health (PGH) is training scientists in these regions to isolate relevant therapeutic phages for pathogenic bacteria within their locality, and thus contributing to making phage technology universally available. Materials and Methods: During the inaugural PGH workshop in East Africa, samples from Ugandan municipal sewage facilities were collected and two novel Escherichia coli lytic phages were isolated and characterized. Results: The phages, UP19 (capsid diameter ∼100 nm, contractile tail ∼120/20 nm) and UP30 (capsid diameter ∼70 nm, noncontractile tail of ∼170/20 nm), lysed ∼82% and ∼36% of the 11 clinical isolates examined, respectively. The genomes of UP19 (171.402 kb, 282 CDS) and UP30 (49.834 kb, 75 CDS) closely match the genera Dhakavirus and Tunavirus, respectively. Conclusion: The phages isolated have therapeutic potential for further development against E. coli infections.

AB - Background: The antimicrobial resistance catastrophe is a growing global health threat and predicted to be worse in developing countries. Phages for Global Health (PGH) is training scientists in these regions to isolate relevant therapeutic phages for pathogenic bacteria within their locality, and thus contributing to making phage technology universally available. Materials and Methods: During the inaugural PGH workshop in East Africa, samples from Ugandan municipal sewage facilities were collected and two novel Escherichia coli lytic phages were isolated and characterized. Results: The phages, UP19 (capsid diameter ∼100 nm, contractile tail ∼120/20 nm) and UP30 (capsid diameter ∼70 nm, noncontractile tail of ∼170/20 nm), lysed ∼82% and ∼36% of the 11 clinical isolates examined, respectively. The genomes of UP19 (171.402 kb, 282 CDS) and UP30 (49.834 kb, 75 CDS) closely match the genera Dhakavirus and Tunavirus, respectively. Conclusion: The phages isolated have therapeutic potential for further development against E. coli infections.

KW - Phages for global health

KW - phage therapy

KW - Escherichia coli phage

KW - antimicrobial resistance

KW - Phages for Global Health

UR - https://www.scopus.com/pages/publications/85173460007

U2 - 10.1089/phage.2023.0012

DO - 10.1089/phage.2023.0012

M3 - Review article

C2 - 37841386

SN - 2641-6530

VL - 4

SP - 141

EP - 149

JO - PHAGE: Therapy, Applications, and Research

JF - PHAGE: Therapy, Applications, and Research

IS - 3

ER -

Novel Escherichia coli-Infecting Bacteriophages Isolated from Uganda That Target Human Clinical Isolates

Abstract

Bibliographical note

Keywords

Access to Document

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