Odd and branched chain fatty acids to estimate proportions of cellulolytic and amylolytic particle associated bacteria

B Vlaeminck, RJ Dewhurst, D Demeyer, V Fievez

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5 Citations (Scopus)

Abstract

Proportions of cellulolytic and amylolytic bacteria in particle associated rumen bacteria were estimated from odd and branched chain fatty acid patterns. Whole rumen contents were collected from four dairy cows and particle associated bacteria were separated by differential centrifugation. The experiment was according to a 4×4 Latin square design with diets differing in forage:concentrate ratio, ranging from 80:20 to 35:65. Proportions of cellulolytic bacteria decreased with increasing dietary proportion of concentrate and estimates were in general agreement with population assessments obtained both by traditional bacterial enumerations and more recent molecular techniques.
Original languageEnglish
Pages (from-to)235-238
JournalJournal of Animal and Feed Sciences
Volume13
Issue number1
Publication statusPrint publication - 2004

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odd chain fatty acids
branched chain fatty acids
bacteria
concentrates
rumen bacteria
plate count
centrifugation
rumen
dairy cows
forage
diet
methodology

Cite this

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title = "Odd and branched chain fatty acids to estimate proportions of cellulolytic and amylolytic particle associated bacteria",
abstract = "Proportions of cellulolytic and amylolytic bacteria in particle associated rumen bacteria were estimated from odd and branched chain fatty acid patterns. Whole rumen contents were collected from four dairy cows and particle associated bacteria were separated by differential centrifugation. The experiment was according to a 4×4 Latin square design with diets differing in forage:concentrate ratio, ranging from 80:20 to 35:65. Proportions of cellulolytic bacteria decreased with increasing dietary proportion of concentrate and estimates were in general agreement with population assessments obtained both by traditional bacterial enumerations and more recent molecular techniques.",
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issn = "1230-1388",
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Odd and branched chain fatty acids to estimate proportions of cellulolytic and amylolytic particle associated bacteria. / Vlaeminck, B; Dewhurst, RJ; Demeyer, D; Fievez, V.

In: Journal of Animal and Feed Sciences, Vol. 13, No. 1, 2004, p. 235-238.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Odd and branched chain fatty acids to estimate proportions of cellulolytic and amylolytic particle associated bacteria

AU - Vlaeminck, B

AU - Dewhurst, RJ

AU - Demeyer, D

AU - Fievez, V

PY - 2004

Y1 - 2004

N2 - Proportions of cellulolytic and amylolytic bacteria in particle associated rumen bacteria were estimated from odd and branched chain fatty acid patterns. Whole rumen contents were collected from four dairy cows and particle associated bacteria were separated by differential centrifugation. The experiment was according to a 4×4 Latin square design with diets differing in forage:concentrate ratio, ranging from 80:20 to 35:65. Proportions of cellulolytic bacteria decreased with increasing dietary proportion of concentrate and estimates were in general agreement with population assessments obtained both by traditional bacterial enumerations and more recent molecular techniques.

AB - Proportions of cellulolytic and amylolytic bacteria in particle associated rumen bacteria were estimated from odd and branched chain fatty acid patterns. Whole rumen contents were collected from four dairy cows and particle associated bacteria were separated by differential centrifugation. The experiment was according to a 4×4 Latin square design with diets differing in forage:concentrate ratio, ranging from 80:20 to 35:65. Proportions of cellulolytic bacteria decreased with increasing dietary proportion of concentrate and estimates were in general agreement with population assessments obtained both by traditional bacterial enumerations and more recent molecular techniques.

UR - https://doi.org/10.22358/jafs/73848/2004

M3 - Article

VL - 13

SP - 235

EP - 238

JO - Journal of Animal and Feed Sciences

JF - Journal of Animal and Feed Sciences

SN - 1230-1388

IS - 1

ER -