Oxidative costs of reproduction in mouse strains selected for different levels of food intake and which differ in reproductive performance

AH Al Jothery, LM Vaanholt, N Mody, A Arnous, J Lykkesfeldt, L Bunger, WG Hill, SE Mitchell, DB Allison, JR Speakman

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Abstract

Oxidative damage caused by reactive oxygen species has been hypothesised to underpin the trade-off between reproduction and somatic maintenance, i.e., the life-history-oxidative stress theory. Previous tests of this hypothesis have proved equivocal, and it has been suggested that the variation in responses may be related to the tissues measured. Here, we measured oxidative damage (protein carbonyls, 8-OHdG) and antioxidant protection (enzymatic antioxidant activity and serum antioxidant capacity) in multiple tissues of reproductive (R) and non-reproductive (N) mice from two mouse strains selectively bred for high (H) or low (L) food intake, which differ in their reproductive performance, i.e., H mice have increased milk energy output (MEO) and wean larger pups. Levels of oxidative damage were unchanged (liver) or reduced (brain and serum) in R versus N mice, and no differences in multiple measures of oxidative protection were found between H and L mice in liver (except for Glutathione Peroxidase), brain or mammary glands. Also, there were no associations between an individual’s energetic investment (e.g., MEO) and most of the oxidative stress measures detected in various tissues. These data are inconsistent with the oxidative stress theory, but were more supportive of, but not completely consistent, with the ‘oxidative shielding’ hypothesis.
Original languageEnglish
JournalScientific Reports
Volume6
Issue number36353
Early online date14 Nov 2016
DOIs
Publication statusFirst published - 14 Nov 2016

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food intake
reproductive performance
mice
oxidative stress
brain
antioxidants
milk
liver
energy
glutathione peroxidase
mammary glands
pups
reactive oxygen species
antioxidant activity
life history
breeds
tissues
proteins
testing

Keywords

  • Biochemistry
  • Genetics

Cite this

Al Jothery, AH ; Vaanholt, LM ; Mody, N ; Arnous, A ; Lykkesfeldt, J ; Bunger, L ; Hill, WG ; Mitchell, SE ; Allison, DB ; Speakman, JR. / Oxidative costs of reproduction in mouse strains selected for different levels of food intake and which differ in reproductive performance. In: Scientific Reports. 2016 ; Vol. 6, No. 36353.
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abstract = "Oxidative damage caused by reactive oxygen species has been hypothesised to underpin the trade-off between reproduction and somatic maintenance, i.e., the life-history-oxidative stress theory. Previous tests of this hypothesis have proved equivocal, and it has been suggested that the variation in responses may be related to the tissues measured. Here, we measured oxidative damage (protein carbonyls, 8-OHdG) and antioxidant protection (enzymatic antioxidant activity and serum antioxidant capacity) in multiple tissues of reproductive (R) and non-reproductive (N) mice from two mouse strains selectively bred for high (H) or low (L) food intake, which differ in their reproductive performance, i.e., H mice have increased milk energy output (MEO) and wean larger pups. Levels of oxidative damage were unchanged (liver) or reduced (brain and serum) in R versus N mice, and no differences in multiple measures of oxidative protection were found between H and L mice in liver (except for Glutathione Peroxidase), brain or mammary glands. Also, there were no associations between an individual’s energetic investment (e.g., MEO) and most of the oxidative stress measures detected in various tissues. These data are inconsistent with the oxidative stress theory, but were more supportive of, but not completely consistent, with the ‘oxidative shielding’ hypothesis.",
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Al Jothery, AH, Vaanholt, LM, Mody, N, Arnous, A, Lykkesfeldt, J, Bunger, L, Hill, WG, Mitchell, SE, Allison, DB & Speakman, JR 2016, 'Oxidative costs of reproduction in mouse strains selected for different levels of food intake and which differ in reproductive performance', Scientific Reports, vol. 6, no. 36353. https://doi.org/10.1038/srep36353

Oxidative costs of reproduction in mouse strains selected for different levels of food intake and which differ in reproductive performance. / Al Jothery, AH; Vaanholt, LM; Mody, N; Arnous, A; Lykkesfeldt, J; Bunger, L; Hill, WG; Mitchell, SE; Allison, DB; Speakman, JR.

In: Scientific Reports, Vol. 6, No. 36353, 14.11.2016.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Oxidative costs of reproduction in mouse strains selected for different levels of food intake and which differ in reproductive performance

AU - Al Jothery, AH

AU - Vaanholt, LM

AU - Mody, N

AU - Arnous, A

AU - Lykkesfeldt, J

AU - Bunger, L

AU - Hill, WG

AU - Mitchell, SE

AU - Allison, DB

AU - Speakman, JR

PY - 2016/11/14

Y1 - 2016/11/14

N2 - Oxidative damage caused by reactive oxygen species has been hypothesised to underpin the trade-off between reproduction and somatic maintenance, i.e., the life-history-oxidative stress theory. Previous tests of this hypothesis have proved equivocal, and it has been suggested that the variation in responses may be related to the tissues measured. Here, we measured oxidative damage (protein carbonyls, 8-OHdG) and antioxidant protection (enzymatic antioxidant activity and serum antioxidant capacity) in multiple tissues of reproductive (R) and non-reproductive (N) mice from two mouse strains selectively bred for high (H) or low (L) food intake, which differ in their reproductive performance, i.e., H mice have increased milk energy output (MEO) and wean larger pups. Levels of oxidative damage were unchanged (liver) or reduced (brain and serum) in R versus N mice, and no differences in multiple measures of oxidative protection were found between H and L mice in liver (except for Glutathione Peroxidase), brain or mammary glands. Also, there were no associations between an individual’s energetic investment (e.g., MEO) and most of the oxidative stress measures detected in various tissues. These data are inconsistent with the oxidative stress theory, but were more supportive of, but not completely consistent, with the ‘oxidative shielding’ hypothesis.

AB - Oxidative damage caused by reactive oxygen species has been hypothesised to underpin the trade-off between reproduction and somatic maintenance, i.e., the life-history-oxidative stress theory. Previous tests of this hypothesis have proved equivocal, and it has been suggested that the variation in responses may be related to the tissues measured. Here, we measured oxidative damage (protein carbonyls, 8-OHdG) and antioxidant protection (enzymatic antioxidant activity and serum antioxidant capacity) in multiple tissues of reproductive (R) and non-reproductive (N) mice from two mouse strains selectively bred for high (H) or low (L) food intake, which differ in their reproductive performance, i.e., H mice have increased milk energy output (MEO) and wean larger pups. Levels of oxidative damage were unchanged (liver) or reduced (brain and serum) in R versus N mice, and no differences in multiple measures of oxidative protection were found between H and L mice in liver (except for Glutathione Peroxidase), brain or mammary glands. Also, there were no associations between an individual’s energetic investment (e.g., MEO) and most of the oxidative stress measures detected in various tissues. These data are inconsistent with the oxidative stress theory, but were more supportive of, but not completely consistent, with the ‘oxidative shielding’ hypothesis.

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JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

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