Population genetic structure of Microdochium majus and Microdochium nivale associated with foot rot of cereals in the Czech Republic and adaptation to penthiopyrad

P Matusinsky, Leona L. Scobodova, ND Havis, Michael Hess, Ludvik Tvaruzek

Research output: Contribution to journalArticle

Abstract

Microdochium nivale and Microdochium majus cause brown foot rot and snow mould in cereals. Even though Microdochium spp. are no longer treated by targeted spraying during the season, they are exposed to untargeted fungicidal applications, while other pathogens are being controlled. The aim of the present study was to evaluate the population genetic structure of Microdochium spp. associated with foot rot of wheat in the Czech Republic and the reaction of that population to the SDHI fungicide penthiopyrad. Using amplified fragment length polymorphism (AFLP) analysis to examine genetic structure, agar dilution to measure inhibitory effect, and various statistical methods to analyse populations, two genetic populations were found corresponding to the species Microdochium majus and M. nivale, and restricted gene flow in populations between years was determined. This study describes for the first time a SDHI-insensitive reaction in populations of M. nivale and M. majus. Low sensitivity was identified in both species, but it was observed more frequently in M. majus populations.
Original languageEnglish
Pages (from-to)1-12
Number of pages12
JournalEuropean Journal of Plant Pathology
Volume155
Issue number1
Early online date27 Aug 2019
DOIs
Publication statusPrint publication - 8 Sep 2019

Fingerprint

Microdochium
Monographella nivalis
Czech Republic
population genetics
snow molds
spraying
amplified fragment length polymorphism
fungicides
gene flow
statistical analysis
agar
wheat
pathogens

Bibliographical note

© Koninklijke Nederlandse Planteziektenkundige Vereniging 2019

Keywords

  • brown foot rot
  • fungicide resistance
  • snow mould
  • wheat

Cite this

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title = "Population genetic structure of Microdochium majus and Microdochium nivale associated with foot rot of cereals in the Czech Republic and adaptation to penthiopyrad",
abstract = "Microdochium nivale and Microdochium majus cause brown foot rot and snow mould in cereals. Even though Microdochium spp. are no longer treated by targeted spraying during the season, they are exposed to untargeted fungicidal applications, while other pathogens are being controlled. The aim of the present study was to evaluate the population genetic structure of Microdochium spp. associated with foot rot of wheat in the Czech Republic and the reaction of that population to the SDHI fungicide penthiopyrad. Using amplified fragment length polymorphism (AFLP) analysis to examine genetic structure, agar dilution to measure inhibitory effect, and various statistical methods to analyse populations, two genetic populations were found corresponding to the species Microdochium majus and M. nivale, and restricted gene flow in populations between years was determined. This study describes for the first time a SDHI-insensitive reaction in populations of M. nivale and M. majus. Low sensitivity was identified in both species, but it was observed more frequently in M. majus populations.",
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Population genetic structure of Microdochium majus and Microdochium nivale associated with foot rot of cereals in the Czech Republic and adaptation to penthiopyrad. / Matusinsky, P; Scobodova, Leona L.; Havis, ND; Hess, Michael; Tvaruzek, Ludvik.

In: European Journal of Plant Pathology, Vol. 155, No. 1, 08.09.2019, p. 1-12.

Research output: Contribution to journalArticle

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T1 - Population genetic structure of Microdochium majus and Microdochium nivale associated with foot rot of cereals in the Czech Republic and adaptation to penthiopyrad

AU - Matusinsky, P

AU - Scobodova, Leona L.

AU - Havis, ND

AU - Hess, Michael

AU - Tvaruzek, Ludvik

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AB - Microdochium nivale and Microdochium majus cause brown foot rot and snow mould in cereals. Even though Microdochium spp. are no longer treated by targeted spraying during the season, they are exposed to untargeted fungicidal applications, while other pathogens are being controlled. The aim of the present study was to evaluate the population genetic structure of Microdochium spp. associated with foot rot of wheat in the Czech Republic and the reaction of that population to the SDHI fungicide penthiopyrad. Using amplified fragment length polymorphism (AFLP) analysis to examine genetic structure, agar dilution to measure inhibitory effect, and various statistical methods to analyse populations, two genetic populations were found corresponding to the species Microdochium majus and M. nivale, and restricted gene flow in populations between years was determined. This study describes for the first time a SDHI-insensitive reaction in populations of M. nivale and M. majus. Low sensitivity was identified in both species, but it was observed more frequently in M. majus populations.

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