Precise annotation of human, chimpanzee, rhesus macaque and mouse mitochondrial genomes leads to insight into mitochondrial transcription in mammals

Xiufeng Jin, Zhi Cheng, Bo Wang, Tung On Yau, Ze Chen, Stephen C Barker, Defu Chen, Wenjun Bu, Daqing Sun, Shan Gao

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

In the present study, we applied our 'precise annotation' to the mitochondrial (mt) genomes of human, chimpanzee, rhesus macaque and mouse using 5' and 3' end small RNAs. Our new annotations updated previous annotations. In particular, our new annotations led to two important novel findings: (1) the identification of five Conserved Sequence Blocks (CSB1, CSB2, CSB3, LSP and HSP) in the control regions; and (2) the annotation of Transcription Initiation and novel Transcription Termination Sites. Based on these annotations, we proposed a novel model of mt transcription which can account for the mt transcription and its regulation in mammals. According to our model, Transcription Termination Sites function as switches to regulate the production of short, long primary transcripts and uninterrupted transcription, rather than simply terminate the mt transcription. Moreover, the expression levels of mitochondrial transcription termination factors control the proportions of rRNAs, mRNAs and lncRNAs in total mt RNA. Our findings point to the existence of many other, as yet unidentified, Transcription Termination Sites in mammals.

Original languageEnglish
Pages (from-to)395-402
Number of pages8
JournalRNA Biology
Volume17
Issue number3
Early online date10 Jan 2020
DOIs
Publication statusPrint publication - 3 Mar 2020
Externally publishedYes

Keywords

  • Mitochondrial DNA
  • control region
  • mitochondrial t ranscription
  • precise annotation
  • uninterrupted transcription

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