Preliminary investigation into a potential role for myostatin and its receptor (ActRIIB) in lean and obese horses and ponies

PK Morrison, Chen Bing, Patricia Harris, Charlotte Maltin, Dai Grove-White, CM Argo*

*Corresponding author for this work

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2 Citations (Scopus)
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Abstract

Obesity is a widespread problem across the leisure population of horses and ponies in industrialised nations. Skeletal muscle is a major contributor to whole body resting energy requirements and communicates with other tissues through the secretion of myokines into the circulation. Myostatin, a myokine and negative regulator of skeletal muscle mass, has been implicated in obesity development in other species. This study evaluated gene and protein expression of myostatin and its receptor, ActRIIB in adipose tissues and skeletal muscles and serum myostatin concentrations in six lean and six obese animals to explore putative associations between these factors and obesity in horses and ponies. Myostatin mRNA expression was increased while ActRIIB mRNA was decreased in skeletal muscles of obese animals but these differences were absent at the protein level. Myostatin mRNA was increased in crest fat of obese animals but neither myostatin nor ActRIIB proteins were detected in this tissue. Mean circulating myostatin concentrations were significantly higher in obese than in lean groups; 4.98 ng/ml (±2.71) and 9.00 ng/ml (±2.04) for the lean and obese groups, respectively. In addition, there was a significant positive association between these levels and myostatin gene expression in skeletal muscles (average R2 = 0.58; p<0.05). Together, these results provide further basis for the speculation that myostatin and its receptor may play a role in obesity in horses and ponies.
Original languageEnglish
Article numbere112621
JournalPLoS ONE
Volume9
Issue number11
Early online date12 Nov 2014
DOIs
Publication statusFirst published - 12 Nov 2014
Externally publishedYes

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Myostatin
myostatin
Horses
horses
receptors
Muscle
skeletal muscle
Skeletal Muscle
obesity
Obesity
Animals
Tissue
Messenger RNA
Gene Expression
gene expression
animals
Proteins
Leisure Activities
Developed Countries
Gene expression

Cite this

Morrison, PK ; Bing, Chen ; Harris, Patricia ; Maltin, Charlotte ; Grove-White, Dai ; Argo, CM. / Preliminary investigation into a potential role for myostatin and its receptor (ActRIIB) in lean and obese horses and ponies. In: PLoS ONE. 2014 ; Vol. 9, No. 11.
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abstract = "Obesity is a widespread problem across the leisure population of horses and ponies in industrialised nations. Skeletal muscle is a major contributor to whole body resting energy requirements and communicates with other tissues through the secretion of myokines into the circulation. Myostatin, a myokine and negative regulator of skeletal muscle mass, has been implicated in obesity development in other species. This study evaluated gene and protein expression of myostatin and its receptor, ActRIIB in adipose tissues and skeletal muscles and serum myostatin concentrations in six lean and six obese animals to explore putative associations between these factors and obesity in horses and ponies. Myostatin mRNA expression was increased while ActRIIB mRNA was decreased in skeletal muscles of obese animals but these differences were absent at the protein level. Myostatin mRNA was increased in crest fat of obese animals but neither myostatin nor ActRIIB proteins were detected in this tissue. Mean circulating myostatin concentrations were significantly higher in obese than in lean groups; 4.98 ng/ml (±2.71) and 9.00 ng/ml (±2.04) for the lean and obese groups, respectively. In addition, there was a significant positive association between these levels and myostatin gene expression in skeletal muscles (average R2 = 0.58; p<0.05). Together, these results provide further basis for the speculation that myostatin and its receptor may play a role in obesity in horses and ponies.",
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Preliminary investigation into a potential role for myostatin and its receptor (ActRIIB) in lean and obese horses and ponies. / Morrison, PK; Bing, Chen; Harris, Patricia; Maltin, Charlotte; Grove-White, Dai; Argo, CM.

In: PLoS ONE, Vol. 9, No. 11, e112621, 12.11.2014.

Research output: Contribution to journalArticleResearchpeer-review

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