Phenotypic and phylogenetic studies were performed on eight Gram-negative-staining, rod-shaped bacteria isolated from seals. Biochemical and physiological studies showed identical profiles for all of the isolates and indicated that they were related to the family Pasteurellaceae. 16S rRNA gene sequencing demonstrated that the organism represented a distinct cluster with two sublines within the family Pasteurellaceae with <96 % sequence similarity to any recognized species. Multilocus sequence analysis (MLSA) including rpoB, infB and recN genes further confirmed these findings with the eight isolates forming a genus-like cluster with two branches. Genome relatedness as deduced from recN gene sequences suggested that the isolates represented a new genus with two species. On the basis of the results of the phylogenetic analysis and phenotypic criteria, it is proposed that these bacteria from seals are classified as Bisgaardia hudsonensis gen. nov., sp. nov. (the type species) and Bisgaardia genomospecies 1. The G+C content of the DNA was 39.5 mol%. The type strain of Bisgaardia hudsonensis gen. nov., sp. nov. is M327/99/2T ( = CCUG 43067T = NCTC 13475T = 98-D-690BT) and the reference strain of Bisgaardia genomospecies 1 is M1765/96/5 ( = CCUG 59551 = NCTC 13474).
|Pages (from-to)||3016 - 3022|
|Number of pages||7|
|Journal||International Journal of Systematic and Evolutionary Microbiology|
|Publication status||First published - 2011|
Foster, G., Higgins, R., Leclair, D., Korczak, BM., Mikaelian, I., Patterson, IAP., & Kuhnert, P. (2011). Proposal of Bisgaardia hudsonensis gen. nov., sp. nov. and an additional genomospecies, isolated from seals, as new members of the family Pasteurellaceae. International Journal of Systematic and Evolutionary Microbiology, 61, 3016 - 3022.