Putative nociceptor responses to mechanical and chemical stimulation in skeletal muscles of the chicken leg

DA Sandercock*

*Corresponding author for this work

Research output: Contribution to journalReview article

8 Citations (Scopus)

Abstract

Electrophysiological responses of nociceptive sensory afferent fibres in the skeletal muscle of the chicken (Gallus domesticus) were examined using mechanical and chemical stimulation. The activity of single nociceptive afferent fibres was recorded from micro-dissected filaments of the fibular and lateral tibial nerves, which innervate the fibularis longus and lateral gastrocnemius muscles. Seventeen putative nociceptive fibres were identified by mechanical stimulation (muscle compression). Conduction velocities (CVs) ranged from 2.8 to 11.3 m/s (mean 5.8; S.E.M.F0.9 m/s). Response thresholds to tissue compression ranged from 38 to 126 kPa (mean 81; S.E.M.±4 kPa). Increases in pressure intensity, above individual fibre thresholds (X2 moderate; X3 noxious), produced intensity dependent increases in discharge rates. Fibres exhibited slowly adapting, irregular discharges lasting the duration of the stimulus and showed no spontaneous activity in the absence of mechanical stimulation. Intramuscular injection of acetic acid (1% v/v in isotonic saline; pH 2.8) in to the receptive field area stimulated discharge activity in 13 of the 17 (76%) pressure sensitive fibres. Acid injection resulted in prolonged irregular single or intermittent clustered discharges, which continued beyond the 15-min recording period. This study demonstrates the existence of nociceptive sensory fibres in chicken skeletal muscle that are able to respond to and encode acute tissue threatening and subjectively painful stimuli. The physiological characteristics of these nociceptive afferents are consistent with mammalian group III skeletal muscle nociceptors. These findings support the suggestion of a common, acute nociceptive response function in skeletal muscle in avians and other vertebrate classes.
Original languageEnglish
Pages (from-to)155-162
Number of pages7
JournalBrain Research Reviews
Volume46
Issue number2
Early online date11 Sep 2004
DOIs
Publication statusPrint publication - Oct 2004
Externally publishedYes

Fingerprint

skeletal muscle
legs
chickens
muscles
intramuscular injection
acetic acid
nerve tissue
vertebrates
injection
duration
acids
tissues
myotubes

Keywords

  • Chicken
  • Skeletal muscle
  • Nociceptive sensory afferents
  • Mechanical stimulation
  • Chemical stimulation
  • Response thresholds
  • Muscle pain
  • Myopathy
  • Muscle injury
  • Avians
  • Neuronal firing
  • Conduction velocities

Cite this

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title = "Putative nociceptor responses to mechanical and chemical stimulation in skeletal muscles of the chicken leg",
abstract = "Electrophysiological responses of nociceptive sensory afferent fibres in the skeletal muscle of the chicken (Gallus domesticus) were examined using mechanical and chemical stimulation. The activity of single nociceptive afferent fibres was recorded from micro-dissected filaments of the fibular and lateral tibial nerves, which innervate the fibularis longus and lateral gastrocnemius muscles. Seventeen putative nociceptive fibres were identified by mechanical stimulation (muscle compression). Conduction velocities (CVs) ranged from 2.8 to 11.3 m/s (mean 5.8; S.E.M.F0.9 m/s). Response thresholds to tissue compression ranged from 38 to 126 kPa (mean 81; S.E.M.±4 kPa). Increases in pressure intensity, above individual fibre thresholds (X2 moderate; X3 noxious), produced intensity dependent increases in discharge rates. Fibres exhibited slowly adapting, irregular discharges lasting the duration of the stimulus and showed no spontaneous activity in the absence of mechanical stimulation. Intramuscular injection of acetic acid (1{\%} v/v in isotonic saline; pH 2.8) in to the receptive field area stimulated discharge activity in 13 of the 17 (76{\%}) pressure sensitive fibres. Acid injection resulted in prolonged irregular single or intermittent clustered discharges, which continued beyond the 15-min recording period. This study demonstrates the existence of nociceptive sensory fibres in chicken skeletal muscle that are able to respond to and encode acute tissue threatening and subjectively painful stimuli. The physiological characteristics of these nociceptive afferents are consistent with mammalian group III skeletal muscle nociceptors. These findings support the suggestion of a common, acute nociceptive response function in skeletal muscle in avians and other vertebrate classes.",
keywords = "Chicken, Skeletal muscle, Nociceptive sensory afferents, Mechanical stimulation, Chemical stimulation, Response thresholds, Muscle pain, Myopathy, Muscle injury, Avians, Neuronal firing, Conduction velocities",
author = "DA Sandercock",
year = "2004",
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language = "English",
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pages = "155--162",
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Putative nociceptor responses to mechanical and chemical stimulation in skeletal muscles of the chicken leg. / Sandercock, DA.

In: Brain Research Reviews, Vol. 46, No. 2, 10.2004, p. 155-162.

Research output: Contribution to journalReview article

TY - JOUR

T1 - Putative nociceptor responses to mechanical and chemical stimulation in skeletal muscles of the chicken leg

AU - Sandercock, DA

PY - 2004/10

Y1 - 2004/10

N2 - Electrophysiological responses of nociceptive sensory afferent fibres in the skeletal muscle of the chicken (Gallus domesticus) were examined using mechanical and chemical stimulation. The activity of single nociceptive afferent fibres was recorded from micro-dissected filaments of the fibular and lateral tibial nerves, which innervate the fibularis longus and lateral gastrocnemius muscles. Seventeen putative nociceptive fibres were identified by mechanical stimulation (muscle compression). Conduction velocities (CVs) ranged from 2.8 to 11.3 m/s (mean 5.8; S.E.M.F0.9 m/s). Response thresholds to tissue compression ranged from 38 to 126 kPa (mean 81; S.E.M.±4 kPa). Increases in pressure intensity, above individual fibre thresholds (X2 moderate; X3 noxious), produced intensity dependent increases in discharge rates. Fibres exhibited slowly adapting, irregular discharges lasting the duration of the stimulus and showed no spontaneous activity in the absence of mechanical stimulation. Intramuscular injection of acetic acid (1% v/v in isotonic saline; pH 2.8) in to the receptive field area stimulated discharge activity in 13 of the 17 (76%) pressure sensitive fibres. Acid injection resulted in prolonged irregular single or intermittent clustered discharges, which continued beyond the 15-min recording period. This study demonstrates the existence of nociceptive sensory fibres in chicken skeletal muscle that are able to respond to and encode acute tissue threatening and subjectively painful stimuli. The physiological characteristics of these nociceptive afferents are consistent with mammalian group III skeletal muscle nociceptors. These findings support the suggestion of a common, acute nociceptive response function in skeletal muscle in avians and other vertebrate classes.

AB - Electrophysiological responses of nociceptive sensory afferent fibres in the skeletal muscle of the chicken (Gallus domesticus) were examined using mechanical and chemical stimulation. The activity of single nociceptive afferent fibres was recorded from micro-dissected filaments of the fibular and lateral tibial nerves, which innervate the fibularis longus and lateral gastrocnemius muscles. Seventeen putative nociceptive fibres were identified by mechanical stimulation (muscle compression). Conduction velocities (CVs) ranged from 2.8 to 11.3 m/s (mean 5.8; S.E.M.F0.9 m/s). Response thresholds to tissue compression ranged from 38 to 126 kPa (mean 81; S.E.M.±4 kPa). Increases in pressure intensity, above individual fibre thresholds (X2 moderate; X3 noxious), produced intensity dependent increases in discharge rates. Fibres exhibited slowly adapting, irregular discharges lasting the duration of the stimulus and showed no spontaneous activity in the absence of mechanical stimulation. Intramuscular injection of acetic acid (1% v/v in isotonic saline; pH 2.8) in to the receptive field area stimulated discharge activity in 13 of the 17 (76%) pressure sensitive fibres. Acid injection resulted in prolonged irregular single or intermittent clustered discharges, which continued beyond the 15-min recording period. This study demonstrates the existence of nociceptive sensory fibres in chicken skeletal muscle that are able to respond to and encode acute tissue threatening and subjectively painful stimuli. The physiological characteristics of these nociceptive afferents are consistent with mammalian group III skeletal muscle nociceptors. These findings support the suggestion of a common, acute nociceptive response function in skeletal muscle in avians and other vertebrate classes.

KW - Chicken

KW - Skeletal muscle

KW - Nociceptive sensory afferents

KW - Mechanical stimulation

KW - Chemical stimulation

KW - Response thresholds

KW - Muscle pain

KW - Myopathy

KW - Muscle injury

KW - Avians

KW - Neuronal firing

KW - Conduction velocities

U2 - https://doi.org/10.1016/j.brainresrev.2004.07.020

DO - https://doi.org/10.1016/j.brainresrev.2004.07.020

M3 - Review article

VL - 46

SP - 155

EP - 162

JO - Brain Research Reviews

JF - Brain Research Reviews

SN - 0165-0173

IS - 2

ER -