QTL mapping for resistance against cereal cyst nematode (Heterodera avenae Woll.) in wheat (Triticum aestivum L.)

Saksham Pundir, Rajiv Sharma, Deepak Kumar, Vikas Kumar Singh, Deepti Chaturvedi, Rambir Singh Kanwar, Marion S. Röder, Andreas Börner, Martin W. Ganal, Pushpendra Kumar Gupta, Shailendra Sharma, Shiveta Sharma*

*Corresponding author for this work

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Abstract

The resistance to cereal cyst nematode (Heterodera avenae Woll.) in wheat (Triticum aestivum L.) was studied using 114 doubled haploid lines from a novel ITMI mapping population. These lines were screened for nematode infestation in a controlled environment for two years. QTL-mapping analyses were performed across two years (Y1 and Y2) as well as combining two years (CY) data. On the 114 lines that were screened, a total of 2,736 data points (genotype, batch or years, and replication combinations) were acquired. For QTL analysis, 12,093 markers (11,678 SNPs and 415 SSRs markers) were used, after filtering the genotypic data, for the QTL mapping. Composite interval mapping, using Haley-Knott regression (hk) method in R/QTL, was used for QTL analysis. In total, 19 QTLs were detected out of which 13 were novel and six were found to be colocalized or nearby to previously reported Cre genes, QTLs or MTAs for H. avenae or H. filipjevi. Nine QTLs were detected across all three groups (Y1, Y2 and CY) including a significant QTL "QCcn.ha-2D" on chromosome 2D that explains 23% of the variance. This QTL colocalized with a previously identified Cre3 locus. Novel QTL, QCcn.ha-2A, detected in the present study could be the possible unreported homeoloci to QCcn.ha-2D, QCcn.ha-2B.1 and QCcn.ha-2B.2. Six significant digenic epistatic interactions were also observed. In addition, 26 candidate genes were also identified including genes known for their involvement in PPNs (plant parasitic nematodes) resistance in different plant species. In-silico expression of putative candidate genes showed differential expression in roots during specific developmental stages. Results obtained in the present study are useful for wheat breeding to generate resistant genetic resources against H. avenae.

Original languageEnglish
Article number9586
JournalScientific Reports
Volume12
Issue number1
Early online date10 Jun 2022
DOIs
Publication statusFirst published - 10 Jun 2022

Keywords

  • Animals
  • Cysts
  • Edible Grain
  • Plant Breeding
  • Plant Diseases/genetics
  • Triticum/genetics
  • Tylenchida
  • Tylenchoidea/genetics

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