Quantitation using informative zeros (QUIZ): Application for GMO detection and quantification without recourse to certified reference material

David Lee, Maurizio La Mura, Andrew Greenland, Ian Mackay

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The amount of genetically modified RoundUp Ready™ soya in admixture samples was determined using a method, quantitation using informative zeros that employs most probable number statistics to estimate molecule numbers. By measuring the numbers of lectin, acting as a reference marker, and GMO-specific targets within these samples, we were able to determine their GMO contents. Estimates derived from QUIZ were comparable with results obtained using conventional real-time Taqman® PCR. Results of GMO content determination using QUIZ is based on a 'per genome' units and estimations can be obtained using very small amounts of DNA. Certified reference materials, constructed using weight measurements, were not required for quantification. © 2008 Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)974-978
Number of pages5
JournalFood Chemistry
Volume118
Issue number4
DOIs
Publication statusPrint publication - 15 Feb 2010
Externally publishedYes

Fingerprint

glyphosate
Weighing
Lectins
Real-Time Polymerase Chain Reaction
Genes
Statistics
Genome
Weights and Measures
Molecules
DNA
lectins
quantitative polymerase chain reaction
statistics
sampling
genome
methodology

Keywords

  • DNA quantitation
  • GMO content determination
  • Lectin
  • RoundUp Ready™ soya
  • Single molecule amplification and detection
  • Transgene

Cite this

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abstract = "The amount of genetically modified RoundUp Ready™ soya in admixture samples was determined using a method, quantitation using informative zeros that employs most probable number statistics to estimate molecule numbers. By measuring the numbers of lectin, acting as a reference marker, and GMO-specific targets within these samples, we were able to determine their GMO contents. Estimates derived from QUIZ were comparable with results obtained using conventional real-time Taqman{\circledR} PCR. Results of GMO content determination using QUIZ is based on a 'per genome' units and estimations can be obtained using very small amounts of DNA. Certified reference materials, constructed using weight measurements, were not required for quantification. {\circledC} 2008 Elsevier Ltd. All rights reserved.",
keywords = "DNA quantitation, GMO content determination, Lectin, RoundUp Ready™ soya, Single molecule amplification and detection, Transgene",
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Quantitation using informative zeros (QUIZ): Application for GMO detection and quantification without recourse to certified reference material. / Lee, David; Mura, Maurizio La; Greenland, Andrew; Mackay, Ian.

In: Food Chemistry, Vol. 118, No. 4, 15.02.2010, p. 974-978.

Research output: Contribution to journalArticle

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