Quantitation using informative zeros (QUIZ): Application for GMO detection and quantification without recourse to certified reference material

David Lee; Maurizio La Mura; Andrew Greenland; Ian Mackay

doi:10.1016/j.foodchem.2008.05.118

Quantitation using informative zeros (QUIZ): Application for GMO detection and quantification without recourse to certified reference material

David Lee, Maurizio La Mura, Andrew Greenland, Ian Mackay

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

The amount of genetically modified RoundUp Ready™ soya in admixture samples was determined using a method, quantitation using informative zeros that employs most probable number statistics to estimate molecule numbers. By measuring the numbers of lectin, acting as a reference marker, and GMO-specific targets within these samples, we were able to determine their GMO contents. Estimates derived from QUIZ were comparable with results obtained using conventional real-time Taqman® PCR. Results of GMO content determination using QUIZ is based on a 'per genome' units and estimations can be obtained using very small amounts of DNA. Certified reference materials, constructed using weight measurements, were not required for quantification. © 2008 Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	974-978
Number of pages	5
Journal	Food Chemistry
Volume	118
Issue number	4
DOIs	https://doi.org/10.1016/j.foodchem.2008.05.118
Publication status	Print publication - 15 Feb 2010
Externally published	Yes

Keywords

DNA quantitation
GMO content determination
Lectin
RoundUp Ready™ soya
Single molecule amplification and detection
Transgene

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10.1016/j.foodchem.2008.05.118

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title = "Quantitation using informative zeros (QUIZ): Application for GMO detection and quantification without recourse to certified reference material",

abstract = "The amount of genetically modified RoundUp Ready{\texttrademark} soya in admixture samples was determined using a method, quantitation using informative zeros that employs most probable number statistics to estimate molecule numbers. By measuring the numbers of lectin, acting as a reference marker, and GMO-specific targets within these samples, we were able to determine their GMO contents. Estimates derived from QUIZ were comparable with results obtained using conventional real-time Taqman{\textregistered} PCR. Results of GMO content determination using QUIZ is based on a 'per genome' units and estimations can be obtained using very small amounts of DNA. Certified reference materials, constructed using weight measurements, were not required for quantification. {\textcopyright} 2008 Elsevier Ltd. All rights reserved.",

keywords = "DNA quantitation, GMO content determination, Lectin, RoundUp Ready{\texttrademark} soya, Single molecule amplification and detection, Transgene",

author = "David Lee and Mura, {Maurizio La} and Andrew Greenland and Ian Mackay",

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doi = "10.1016/j.foodchem.2008.05.118",

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T1 - Quantitation using informative zeros (QUIZ): Application for GMO detection and quantification without recourse to certified reference material

AU - Lee, David

AU - Mura, Maurizio La

AU - Greenland, Andrew

AU - Mackay, Ian

PY - 2010/2/15

Y1 - 2010/2/15

N2 - The amount of genetically modified RoundUp Ready™ soya in admixture samples was determined using a method, quantitation using informative zeros that employs most probable number statistics to estimate molecule numbers. By measuring the numbers of lectin, acting as a reference marker, and GMO-specific targets within these samples, we were able to determine their GMO contents. Estimates derived from QUIZ were comparable with results obtained using conventional real-time Taqman® PCR. Results of GMO content determination using QUIZ is based on a 'per genome' units and estimations can be obtained using very small amounts of DNA. Certified reference materials, constructed using weight measurements, were not required for quantification. © 2008 Elsevier Ltd. All rights reserved.

AB - The amount of genetically modified RoundUp Ready™ soya in admixture samples was determined using a method, quantitation using informative zeros that employs most probable number statistics to estimate molecule numbers. By measuring the numbers of lectin, acting as a reference marker, and GMO-specific targets within these samples, we were able to determine their GMO contents. Estimates derived from QUIZ were comparable with results obtained using conventional real-time Taqman® PCR. Results of GMO content determination using QUIZ is based on a 'per genome' units and estimations can be obtained using very small amounts of DNA. Certified reference materials, constructed using weight measurements, were not required for quantification. © 2008 Elsevier Ltd. All rights reserved.

KW - DNA quantitation

KW - GMO content determination

KW - Lectin

KW - RoundUp Ready™ soya

KW - Single molecule amplification and detection

KW - Transgene

UR - http://www.mendeley.com/research/quantitation-using-informative-zeros-quiz-application-gmo-detection-quantification-without-recourse-1

U2 - 10.1016/j.foodchem.2008.05.118

DO - 10.1016/j.foodchem.2008.05.118

M3 - Article

SN - 0308-8146

VL - 118

SP - 974

EP - 978

JO - Food Chemistry

JF - Food Chemistry

IS - 4

ER -

Quantitation using informative zeros (QUIZ): Application for GMO detection and quantification without recourse to certified reference material

Abstract

Keywords

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