Rapid RAPD analysis for distinguishing Listeria species and Listeria monocytogenes serotypes using a capillary air thermal cycler

S. F. Black, D. I. Gray, D. R. Fenlon, R. G. Kroll*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Using a novel capillary thermal cycler, randomly amplified polymorphic DNA (RAPD) generated DNA fingerprints were obtained in 3 h. The RAPD profiles were produced using a random 10‐mer primer (5′‐ACCGCCTGCT‐3′) which discriminated between different Listeria spp. Unique fragment profiles of Listeria monocytogenes serotypes were produced from serotypes 1a, 2, 3a, 4ab, 4a and 4c but serotypes, 1/2a, 4b, 4d and 7 had similar profiles.

Original languageEnglish
Pages (from-to)188-190
Number of pages3
JournalLetters in Applied Microbiology
Volume20
Issue number3
DOIs
Publication statusPrint publication - Mar 1995

Fingerprint Dive into the research topics of 'Rapid RAPD analysis for distinguishing Listeria species and Listeria monocytogenes serotypes using a capillary air thermal cycler'. Together they form a unique fingerprint.

Cite this