Refinement of passive nematode recovery from cotton growing high clay content Australian vertisols

O. G.G. Knox*, B. S. Griffiths

*Corresponding author for this work

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Vertisols are characterized by their high clay content, which can hinder assessment of their soil biology. Recovery of nematodes from vertisols with active techniques has indicated that free living nematode numbers within Australian vertisols are often low. Despite this, cotton yield losses due to the reniform nematode have recently been reported in Australia. In light of this and other changes in the cotton production system, we undertook a series of experiments to determine whether passive nematode extraction techniques could be applied to high clay vertisols. Existing published passive nematode recovery methods proved to be inefficient. The use of sodium hexametaphosphate as a clay dispersant was found to further reduce effectiveness. Instead, direct extraction of soil in Ludox at 1.1 g/cm3 followed by sieving was found to be as effective at recovering nematodes using Baermann funnel extraction from fresh soil but considerably quicker and three times more effective with dried soil.

Original languageEnglish
Pages (from-to)316-325
Number of pages10
JournalCommunications in Soil Science and Plant Analysis
Volume48
Issue number3
Early online date1 Mar 2017
DOIs
Publication statusFirst published - 1 Mar 2017

Fingerprint

Vertisol
Vertisols
nematode
cotton
clay
Nematoda
soil biology
soil
free-living nematodes
sieving
recovery method
dispersant
production technology
production system
methodology
sodium
experiment

Keywords

  • Acrobeles
  • Axonchium
  • Colloidal silica
  • Ludox
  • Pratylenchus

Cite this

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title = "Refinement of passive nematode recovery from cotton growing high clay content Australian vertisols",
abstract = "Vertisols are characterized by their high clay content, which can hinder assessment of their soil biology. Recovery of nematodes from vertisols with active techniques has indicated that free living nematode numbers within Australian vertisols are often low. Despite this, cotton yield losses due to the reniform nematode have recently been reported in Australia. In light of this and other changes in the cotton production system, we undertook a series of experiments to determine whether passive nematode extraction techniques could be applied to high clay vertisols. Existing published passive nematode recovery methods proved to be inefficient. The use of sodium hexametaphosphate as a clay dispersant was found to further reduce effectiveness. Instead, direct extraction of soil in Ludox at 1.1 g/cm3 followed by sieving was found to be as effective at recovering nematodes using Baermann funnel extraction from fresh soil but considerably quicker and three times more effective with dried soil.",
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Refinement of passive nematode recovery from cotton growing high clay content Australian vertisols. / Knox, O. G.G.; Griffiths, B. S.

In: Communications in Soil Science and Plant Analysis, Vol. 48, No. 3, 01.03.2017, p. 316-325.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Refinement of passive nematode recovery from cotton growing high clay content Australian vertisols

AU - Knox, O. G.G.

AU - Griffiths, B. S.

PY - 2017/3/1

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AB - Vertisols are characterized by their high clay content, which can hinder assessment of their soil biology. Recovery of nematodes from vertisols with active techniques has indicated that free living nematode numbers within Australian vertisols are often low. Despite this, cotton yield losses due to the reniform nematode have recently been reported in Australia. In light of this and other changes in the cotton production system, we undertook a series of experiments to determine whether passive nematode extraction techniques could be applied to high clay vertisols. Existing published passive nematode recovery methods proved to be inefficient. The use of sodium hexametaphosphate as a clay dispersant was found to further reduce effectiveness. Instead, direct extraction of soil in Ludox at 1.1 g/cm3 followed by sieving was found to be as effective at recovering nematodes using Baermann funnel extraction from fresh soil but considerably quicker and three times more effective with dried soil.

KW - Acrobeles

KW - Axonchium

KW - Colloidal silica

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KW - Pratylenchus

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