Relationships among cultivated and wild lentils revealed by RAPD analysis

S. K. Sharma, I. K. Dawson, R. Waugh

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

RAPD markers were used to distinguish between six different Lens taxa, representing cultivated lentil and its wild relatives. Twenty-four arbitrary sequence 10-mer primers were identified which revealed robust and easily interpretable amplification-product profiles. These generated a total of 88 polymorphic bands in 54 accessions and were used to partition variation within and among Lens taxa. The data showed that, of the taxa examined, ssp. orientalis is most similar to cultivated lentil. L. ervoides was the most divergent wild taxon followed by L. nigricans. The genetic similarity between the latter two species was of the same magnitude as between ssp. orientalis and cultivated lentil. In addition, species-diagnostic amplification products specific to L. odemensis, L. ervoides and L. nigricans were identified. These results correspond well with previous isozyme and RFLP studies. RAPDs, however, appear to provide a greater degree of resolution at a sub-species level. The level of variation detected within cultivated lentils suggests that RAPD markers may be an appropriate technology for the construction of genetic linkage maps between closely related Lens accessions.
Original languageEnglish
Pages (from-to)647-654
Number of pages8
JournalTheoretical and Applied Genetics
Volume91
Issue number4
DOIs
Publication statusPrint publication - Sep 1995
Externally publishedYes

Keywords

  • Cluster analysis
  • Diversity
  • Lens
  • Phylogeny
  • RAPD

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