Relationships between play and responses to tickling in male juvenile rats

TJH Hammond, Vincent Bombail, Birte L Nielsen, Simone L Meddle, AB Lawrence, Sarah M Brown

Research output: Contribution to journalArticle

Abstract

Play is a putatively positive experience and of key interest to the study of affective state in animals. Rats produce 50kHz ultrasonic vocalisation (USVs) during positive experiences, including social play and tickling. The tickling paradigm is intended to mimic social play resulting in positively valanced ultrasonic vocalisation (USV) production. We tested two hypotheses on the relationship between tickling and play: that tickling would increase play behaviour or that play behaviour would increase in anticipation of tickling, and that tickling would share some specific properties of play(rebound and emotional contagion of unexposed cage mates). Male Wistar rats (N=64, with 32 rats/cohort) of 28 days of age were housed in pairs with one rat assigned to be tickled and one as the non-tickled control. Production of 50kHz USVs and hand-following behaviour was measured. Prior to handling, solitary and social play was recorded for 5 minutes in the home cage. A two-day break in tickling was used to assess a potential rebound increase in responses to tickling. Only one rat within each cage was handled to assess emotional contagion through changes in the behaviour of the cage-mate. Solitary but not social play increased prior to tickling relative to controls (p = 0.01).There were marked differences between cohorts; tickled rats in C2 produced less 50kHz USVs than those in C1(p = 0.04) and overall, C2 rats played less than rats in C1 (social p= 0.04 and solitary p< 0.001) and had a lighter start weight on arrival (p= 0.009) compared with cohort 1 (C1).In C1, there was evidence of rebound in USV production (p< 0.001) and a contagious effect of tickling reflected by increased hand-following in cage mates (p = 0.02). We found a positive relationship between start weight and USV responses to tickling (Rs= 0.43, p < 0.001), suggesting that the divergence in USV production may be due to developmental differences between cohorts. The results suggest that the relationship between tickling and play is complex in that tickling only affected solitary and not social play, and that tickling responses showed rebound and contagion effects on cage-mates which were specific to cohort responses to tickling.
Original languageEnglish
Article number104879
JournalApplied Animal Behaviour Science
Volume221
Early online date25 Sep 2019
DOIs
Publication statusPrint publication - Dec 2019

Fingerprint

Ultrasonics
vocalization
ultrasonics
cages
rats
play activities
Hand
hands
Weights and Measures
behavior change
Wistar Rats
animals

Keywords

  • Male rats
  • Play
  • Positive affective states
  • Positive animal welfare
  • Tickling

Cite this

Hammond, TJH ; Bombail, Vincent ; Nielsen, Birte L ; Meddle, Simone L ; Lawrence, AB ; Brown, Sarah M. / Relationships between play and responses to tickling in male juvenile rats. In: Applied Animal Behaviour Science. 2019 ; Vol. 221.
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title = "Relationships between play and responses to tickling in male juvenile rats",
abstract = "Play is a putatively positive experience and of key interest to the study of affective state in animals. Rats produce 50kHz ultrasonic vocalisation (USVs) during positive experiences, including social play and tickling. The tickling paradigm is intended to mimic social play resulting in positively valanced ultrasonic vocalisation (USV) production. We tested two hypotheses on the relationship between tickling and play: that tickling would increase play behaviour or that play behaviour would increase in anticipation of tickling, and that tickling would share some specific properties of play(rebound and emotional contagion of unexposed cage mates). Male Wistar rats (N=64, with 32 rats/cohort) of 28 days of age were housed in pairs with one rat assigned to be tickled and one as the non-tickled control. Production of 50kHz USVs and hand-following behaviour was measured. Prior to handling, solitary and social play was recorded for 5 minutes in the home cage. A two-day break in tickling was used to assess a potential rebound increase in responses to tickling. Only one rat within each cage was handled to assess emotional contagion through changes in the behaviour of the cage-mate. Solitary but not social play increased prior to tickling relative to controls (p = 0.01).There were marked differences between cohorts; tickled rats in C2 produced less 50kHz USVs than those in C1(p = 0.04) and overall, C2 rats played less than rats in C1 (social p= 0.04 and solitary p< 0.001) and had a lighter start weight on arrival (p= 0.009) compared with cohort 1 (C1).In C1, there was evidence of rebound in USV production (p< 0.001) and a contagious effect of tickling reflected by increased hand-following in cage mates (p = 0.02). We found a positive relationship between start weight and USV responses to tickling (Rs= 0.43, p < 0.001), suggesting that the divergence in USV production may be due to developmental differences between cohorts. The results suggest that the relationship between tickling and play is complex in that tickling only affected solitary and not social play, and that tickling responses showed rebound and contagion effects on cage-mates which were specific to cohort responses to tickling.",
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Relationships between play and responses to tickling in male juvenile rats. / Hammond, TJH; Bombail, Vincent; Nielsen, Birte L; Meddle, Simone L; Lawrence, AB; Brown, Sarah M.

In: Applied Animal Behaviour Science, Vol. 221, 104879, 12.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Relationships between play and responses to tickling in male juvenile rats

AU - Hammond, TJH

AU - Bombail, Vincent

AU - Nielsen, Birte L

AU - Meddle, Simone L

AU - Lawrence, AB

AU - Brown, Sarah M

PY - 2019/12

Y1 - 2019/12

N2 - Play is a putatively positive experience and of key interest to the study of affective state in animals. Rats produce 50kHz ultrasonic vocalisation (USVs) during positive experiences, including social play and tickling. The tickling paradigm is intended to mimic social play resulting in positively valanced ultrasonic vocalisation (USV) production. We tested two hypotheses on the relationship between tickling and play: that tickling would increase play behaviour or that play behaviour would increase in anticipation of tickling, and that tickling would share some specific properties of play(rebound and emotional contagion of unexposed cage mates). Male Wistar rats (N=64, with 32 rats/cohort) of 28 days of age were housed in pairs with one rat assigned to be tickled and one as the non-tickled control. Production of 50kHz USVs and hand-following behaviour was measured. Prior to handling, solitary and social play was recorded for 5 minutes in the home cage. A two-day break in tickling was used to assess a potential rebound increase in responses to tickling. Only one rat within each cage was handled to assess emotional contagion through changes in the behaviour of the cage-mate. Solitary but not social play increased prior to tickling relative to controls (p = 0.01).There were marked differences between cohorts; tickled rats in C2 produced less 50kHz USVs than those in C1(p = 0.04) and overall, C2 rats played less than rats in C1 (social p= 0.04 and solitary p< 0.001) and had a lighter start weight on arrival (p= 0.009) compared with cohort 1 (C1).In C1, there was evidence of rebound in USV production (p< 0.001) and a contagious effect of tickling reflected by increased hand-following in cage mates (p = 0.02). We found a positive relationship between start weight and USV responses to tickling (Rs= 0.43, p < 0.001), suggesting that the divergence in USV production may be due to developmental differences between cohorts. The results suggest that the relationship between tickling and play is complex in that tickling only affected solitary and not social play, and that tickling responses showed rebound and contagion effects on cage-mates which were specific to cohort responses to tickling.

AB - Play is a putatively positive experience and of key interest to the study of affective state in animals. Rats produce 50kHz ultrasonic vocalisation (USVs) during positive experiences, including social play and tickling. The tickling paradigm is intended to mimic social play resulting in positively valanced ultrasonic vocalisation (USV) production. We tested two hypotheses on the relationship between tickling and play: that tickling would increase play behaviour or that play behaviour would increase in anticipation of tickling, and that tickling would share some specific properties of play(rebound and emotional contagion of unexposed cage mates). Male Wistar rats (N=64, with 32 rats/cohort) of 28 days of age were housed in pairs with one rat assigned to be tickled and one as the non-tickled control. Production of 50kHz USVs and hand-following behaviour was measured. Prior to handling, solitary and social play was recorded for 5 minutes in the home cage. A two-day break in tickling was used to assess a potential rebound increase in responses to tickling. Only one rat within each cage was handled to assess emotional contagion through changes in the behaviour of the cage-mate. Solitary but not social play increased prior to tickling relative to controls (p = 0.01).There were marked differences between cohorts; tickled rats in C2 produced less 50kHz USVs than those in C1(p = 0.04) and overall, C2 rats played less than rats in C1 (social p= 0.04 and solitary p< 0.001) and had a lighter start weight on arrival (p= 0.009) compared with cohort 1 (C1).In C1, there was evidence of rebound in USV production (p< 0.001) and a contagious effect of tickling reflected by increased hand-following in cage mates (p = 0.02). We found a positive relationship between start weight and USV responses to tickling (Rs= 0.43, p < 0.001), suggesting that the divergence in USV production may be due to developmental differences between cohorts. The results suggest that the relationship between tickling and play is complex in that tickling only affected solitary and not social play, and that tickling responses showed rebound and contagion effects on cage-mates which were specific to cohort responses to tickling.

KW - Male rats

KW - Play

KW - Positive affective states

KW - Positive animal welfare

KW - Tickling

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DO - 10.1016/j.applanim.2019.104879

M3 - Article

VL - 221

JO - Applied Animal Behaviour Science

JF - Applied Animal Behaviour Science

SN - 0168-1591

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