Relationships between responses to tickling, play behaviour and physical condition in male juvenile rats

TJH Hammond, Sarah M Brown, Simone L Meddle, Vincent Bombail, AB Lawrence

Research output: Contribution to conferenceAbstract

Abstract

The tickling paradigm is a well-established model used to elicit positive emotional states in rats, but there is little investigation into the model’s relationship with play behaviour and how pre-existing characteristics (eg physical condition) influence the response of individuals to tickling. 64 adolescent (age 28 days) male Wistar rats, (split into two batches) were housed in pairs and assigned to treatment (tickling) or control (neutral handling). One animal within each cage (n = 32, 16 per cohort) was handled. During handling, frequency modulated ultrasonic vocalisation (FM USV) production and approach behaviour in the form of hand-following (HF) was measured. Prior to handling, home cage play was recorded for 5 minutes. There were significant cohort effects in tickling responses, play and body weight. In cohort 1 (C1), tickled animals produced more FM USVs (F1,89.1 36.18, p < 0.001) and showed increased HF compared with controls (F1,88.7 = 16.46, p < 0.001), both measures indicating that tickled animals were in a more positive emotional state. In cohort 2 (C2), treatment had no effect on FM USVs (F1,94= 0.44, p = 0.508). However, there was no difference in HF between batches (F1,188 = 0, p = 0.948) with tickled animals also showing increased solitary play (F1,94= 5.03, p = 0.027). Compared with C1, C2 produced fewer tickle-induced FM USVs (F1,94 = 6.98, p = 0.01), had a lower starting body weight (F1,188 = 50.25, p < 0.001), reduced average daily gain (F1,188 = 35.95, p = 0.008) and also showed less solitary and social play (solitary; F1,94= 5.74, p = 0.019: social; F1,94= 3.37, p = 0.069). These results suggest some degree of dissociation between tickling responses and play behaviour; whilst solitary play was increased by tickling, social play was not. Measures of the tickling response were also dissociated as whilst USVs and HF responded to treatment, only USVs were sensitive to a cohort effect. Ultimately, this suggests that the relationship between tickling and play behaviour is more complex than previously described.
Original languageEnglish
Pages65
Number of pages1
Publication statusPrint publication - 2018
EventUFAW International Animal Welfare Science Symposium 2018: Recent advances in animal welfare science VI - Centre for Life, Newcastle upon Tyne, United Kingdom
Duration: 28 Jun 2018 → …
https://www.ufaw.org.uk/ufaw-events/recent-advances-in-animal-welfare-science-vi

Conference

ConferenceUFAW International Animal Welfare Science Symposium 2018
CountryUnited Kingdom
CityNewcastle upon Tyne
Period28/06/18 → …
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play activities
hands
rats
animals
cages
body weight
vocalization
average daily gain
ultrasonics

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Hammond, TJH., Brown, S. M., Meddle, S. L., Bombail, V., & Lawrence, AB. (2018). Relationships between responses to tickling, play behaviour and physical condition in male juvenile rats. 65. Abstract from UFAW International Animal Welfare Science Symposium 2018, Newcastle upon Tyne, United Kingdom.
Hammond, TJH ; Brown, Sarah M ; Meddle, Simone L ; Bombail, Vincent ; Lawrence, AB. / Relationships between responses to tickling, play behaviour and physical condition in male juvenile rats. Abstract from UFAW International Animal Welfare Science Symposium 2018, Newcastle upon Tyne, United Kingdom.1 p.
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abstract = "The tickling paradigm is a well-established model used to elicit positive emotional states in rats, but there is little investigation into the model’s relationship with play behaviour and how pre-existing characteristics (eg physical condition) influence the response of individuals to tickling. 64 adolescent (age 28 days) male Wistar rats, (split into two batches) were housed in pairs and assigned to treatment (tickling) or control (neutral handling). One animal within each cage (n = 32, 16 per cohort) was handled. During handling, frequency modulated ultrasonic vocalisation (FM USV) production and approach behaviour in the form of hand-following (HF) was measured. Prior to handling, home cage play was recorded for 5 minutes. There were significant cohort effects in tickling responses, play and body weight. In cohort 1 (C1), tickled animals produced more FM USVs (F1,89.1 36.18, p < 0.001) and showed increased HF compared with controls (F1,88.7 = 16.46, p < 0.001), both measures indicating that tickled animals were in a more positive emotional state. In cohort 2 (C2), treatment had no effect on FM USVs (F1,94= 0.44, p = 0.508). However, there was no difference in HF between batches (F1,188 = 0, p = 0.948) with tickled animals also showing increased solitary play (F1,94= 5.03, p = 0.027). Compared with C1, C2 produced fewer tickle-induced FM USVs (F1,94 = 6.98, p = 0.01), had a lower starting body weight (F1,188 = 50.25, p < 0.001), reduced average daily gain (F1,188 = 35.95, p = 0.008) and also showed less solitary and social play (solitary; F1,94= 5.74, p = 0.019: social; F1,94= 3.37, p = 0.069). These results suggest some degree of dissociation between tickling responses and play behaviour; whilst solitary play was increased by tickling, social play was not. Measures of the tickling response were also dissociated as whilst USVs and HF responded to treatment, only USVs were sensitive to a cohort effect. Ultimately, this suggests that the relationship between tickling and play behaviour is more complex than previously described.",
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Hammond, TJH, Brown, SM, Meddle, SL, Bombail, V & Lawrence, AB 2018, 'Relationships between responses to tickling, play behaviour and physical condition in male juvenile rats', UFAW International Animal Welfare Science Symposium 2018, Newcastle upon Tyne, United Kingdom, 28/06/18 pp. 65.

Relationships between responses to tickling, play behaviour and physical condition in male juvenile rats. / Hammond, TJH; Brown, Sarah M; Meddle, Simone L; Bombail, Vincent; Lawrence, AB.

2018. 65 Abstract from UFAW International Animal Welfare Science Symposium 2018, Newcastle upon Tyne, United Kingdom.

Research output: Contribution to conferenceAbstract

TY - CONF

T1 - Relationships between responses to tickling, play behaviour and physical condition in male juvenile rats

AU - Hammond, TJH

AU - Brown, Sarah M

AU - Meddle, Simone L

AU - Bombail, Vincent

AU - Lawrence, AB

PY - 2018

Y1 - 2018

N2 - The tickling paradigm is a well-established model used to elicit positive emotional states in rats, but there is little investigation into the model’s relationship with play behaviour and how pre-existing characteristics (eg physical condition) influence the response of individuals to tickling. 64 adolescent (age 28 days) male Wistar rats, (split into two batches) were housed in pairs and assigned to treatment (tickling) or control (neutral handling). One animal within each cage (n = 32, 16 per cohort) was handled. During handling, frequency modulated ultrasonic vocalisation (FM USV) production and approach behaviour in the form of hand-following (HF) was measured. Prior to handling, home cage play was recorded for 5 minutes. There were significant cohort effects in tickling responses, play and body weight. In cohort 1 (C1), tickled animals produced more FM USVs (F1,89.1 36.18, p < 0.001) and showed increased HF compared with controls (F1,88.7 = 16.46, p < 0.001), both measures indicating that tickled animals were in a more positive emotional state. In cohort 2 (C2), treatment had no effect on FM USVs (F1,94= 0.44, p = 0.508). However, there was no difference in HF between batches (F1,188 = 0, p = 0.948) with tickled animals also showing increased solitary play (F1,94= 5.03, p = 0.027). Compared with C1, C2 produced fewer tickle-induced FM USVs (F1,94 = 6.98, p = 0.01), had a lower starting body weight (F1,188 = 50.25, p < 0.001), reduced average daily gain (F1,188 = 35.95, p = 0.008) and also showed less solitary and social play (solitary; F1,94= 5.74, p = 0.019: social; F1,94= 3.37, p = 0.069). These results suggest some degree of dissociation between tickling responses and play behaviour; whilst solitary play was increased by tickling, social play was not. Measures of the tickling response were also dissociated as whilst USVs and HF responded to treatment, only USVs were sensitive to a cohort effect. Ultimately, this suggests that the relationship between tickling and play behaviour is more complex than previously described.

AB - The tickling paradigm is a well-established model used to elicit positive emotional states in rats, but there is little investigation into the model’s relationship with play behaviour and how pre-existing characteristics (eg physical condition) influence the response of individuals to tickling. 64 adolescent (age 28 days) male Wistar rats, (split into two batches) were housed in pairs and assigned to treatment (tickling) or control (neutral handling). One animal within each cage (n = 32, 16 per cohort) was handled. During handling, frequency modulated ultrasonic vocalisation (FM USV) production and approach behaviour in the form of hand-following (HF) was measured. Prior to handling, home cage play was recorded for 5 minutes. There were significant cohort effects in tickling responses, play and body weight. In cohort 1 (C1), tickled animals produced more FM USVs (F1,89.1 36.18, p < 0.001) and showed increased HF compared with controls (F1,88.7 = 16.46, p < 0.001), both measures indicating that tickled animals were in a more positive emotional state. In cohort 2 (C2), treatment had no effect on FM USVs (F1,94= 0.44, p = 0.508). However, there was no difference in HF between batches (F1,188 = 0, p = 0.948) with tickled animals also showing increased solitary play (F1,94= 5.03, p = 0.027). Compared with C1, C2 produced fewer tickle-induced FM USVs (F1,94 = 6.98, p = 0.01), had a lower starting body weight (F1,188 = 50.25, p < 0.001), reduced average daily gain (F1,188 = 35.95, p = 0.008) and also showed less solitary and social play (solitary; F1,94= 5.74, p = 0.019: social; F1,94= 3.37, p = 0.069). These results suggest some degree of dissociation between tickling responses and play behaviour; whilst solitary play was increased by tickling, social play was not. Measures of the tickling response were also dissociated as whilst USVs and HF responded to treatment, only USVs were sensitive to a cohort effect. Ultimately, this suggests that the relationship between tickling and play behaviour is more complex than previously described.

UR - https://www.ufaw.org.uk/ufaw-events/recent-advances-in-animal-welfare-science-vi

M3 - Abstract

SP - 65

ER -

Hammond TJH, Brown SM, Meddle SL, Bombail V, Lawrence AB. Relationships between responses to tickling, play behaviour and physical condition in male juvenile rats. 2018. Abstract from UFAW International Animal Welfare Science Symposium 2018, Newcastle upon Tyne, United Kingdom.