Reliably colonising broiler chickens with Campylobacter spp. using a litter-based method

V Sandilands, F Whyte, LK Williams, TS Wilkinson, NHC Sparks, TJ Humphrey

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Abstract

1. Chicken-associated Campylobacter spp. are the cause of most food poisoning cases in Europe. In order to study the host-pathogen interactions, a reliable and reproducible method of colonising chickens with the bacteria is required. 2. This study aimed to identify a more appropriate and less invasive method of colonisation (cf. gavaging) by seeding bedding material (litter) that commercial chickens are kept on with a mixture of Campylobacter spp., broth and faeces. 3. The first phase of the study tested the longevity of Campylobacter spp. recovery in seeded litter over 24 h: significantly more Campylobacter spp. was recovered at 0 or 3 h post-seeding than at 6 and 24 h post-seeding, indicating that the pathogen can survive to detectable levels for at least 3 h in this environment. 4. In the second phase, three groups of 10 broiler chickens (negative for Campylobacter spp. prior to exposure) were exposed at 21 days of age to one of three different Campylobacter jejuni and C. coli mixes (A, B, C), using the method above. At 28 days of age, birds were euthanised by overdose of barbiturate or cervical dislocation, and livers and caeca removed for Campylobacte spp. assessment. 5. All liver and 28/30 caeca samples tested positive for Campylobacte spp., with mix A and C giving higher counts in the caeca than mix B. The method of euthanasia did not affect Campylobacter spp. counts. 6. In conclusion, a successful method for reliably colonising broiler chickens with Campylobacter spp. has been developed which negates the need for gavaging and is more representative of how contamination occurs in the field.
Original languageEnglish
Pages (from-to)698 - 702
Number of pages5
JournalBritish Poultry Science
Volume59
Issue number6
Early online date21 Sep 2018
DOIs
Publication statusFirst published - 21 Sep 2018

Fingerprint

Campylobacter
broiler chickens
cecum
sowing
chickens
methodology
cervical dislocation
barbiturates
overdose
Campylobacter coli
liver
host-pathogen relationships
Campylobacter jejuni
euthanasia
foodborne illness
feces
pathogens
birds
bacteria

Keywords

  • Caeca
  • Contamination
  • Food-borne illness
  • Gavage
  • Liver
  • Seeding

Cite this

Sandilands, V ; Whyte, F ; Williams, LK ; Wilkinson, TS ; Sparks, NHC ; Humphrey, TJ. / Reliably colonising broiler chickens with Campylobacter spp. using a litter-based method. In: British Poultry Science. 2018 ; Vol. 59, No. 6. pp. 698 - 702.
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abstract = "1. Chicken-associated Campylobacter spp. are the cause of most food poisoning cases in Europe. In order to study the host-pathogen interactions, a reliable and reproducible method of colonising chickens with the bacteria is required. 2. This study aimed to identify a more appropriate and less invasive method of colonisation (cf. gavaging) by seeding bedding material (litter) that commercial chickens are kept on with a mixture of Campylobacter spp., broth and faeces. 3. The first phase of the study tested the longevity of Campylobacter spp. recovery in seeded litter over 24 h: significantly more Campylobacter spp. was recovered at 0 or 3 h post-seeding than at 6 and 24 h post-seeding, indicating that the pathogen can survive to detectable levels for at least 3 h in this environment. 4. In the second phase, three groups of 10 broiler chickens (negative for Campylobacter spp. prior to exposure) were exposed at 21 days of age to one of three different Campylobacter jejuni and C. coli mixes (A, B, C), using the method above. At 28 days of age, birds were euthanised by overdose of barbiturate or cervical dislocation, and livers and caeca removed for Campylobacte spp. assessment. 5. All liver and 28/30 caeca samples tested positive for Campylobacte spp., with mix A and C giving higher counts in the caeca than mix B. The method of euthanasia did not affect Campylobacter spp. counts. 6. In conclusion, a successful method for reliably colonising broiler chickens with Campylobacter spp. has been developed which negates the need for gavaging and is more representative of how contamination occurs in the field.",
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Reliably colonising broiler chickens with Campylobacter spp. using a litter-based method. / Sandilands, V; Whyte, F; Williams, LK; Wilkinson, TS; Sparks, NHC; Humphrey, TJ.

In: British Poultry Science, Vol. 59, No. 6, 21.09.2018, p. 698 - 702.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Reliably colonising broiler chickens with Campylobacter spp. using a litter-based method

AU - Sandilands, V

AU - Whyte, F

AU - Williams, LK

AU - Wilkinson, TS

AU - Sparks, NHC

AU - Humphrey, TJ

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Y1 - 2018/9/21

N2 - 1. Chicken-associated Campylobacter spp. are the cause of most food poisoning cases in Europe. In order to study the host-pathogen interactions, a reliable and reproducible method of colonising chickens with the bacteria is required. 2. This study aimed to identify a more appropriate and less invasive method of colonisation (cf. gavaging) by seeding bedding material (litter) that commercial chickens are kept on with a mixture of Campylobacter spp., broth and faeces. 3. The first phase of the study tested the longevity of Campylobacter spp. recovery in seeded litter over 24 h: significantly more Campylobacter spp. was recovered at 0 or 3 h post-seeding than at 6 and 24 h post-seeding, indicating that the pathogen can survive to detectable levels for at least 3 h in this environment. 4. In the second phase, three groups of 10 broiler chickens (negative for Campylobacter spp. prior to exposure) were exposed at 21 days of age to one of three different Campylobacter jejuni and C. coli mixes (A, B, C), using the method above. At 28 days of age, birds were euthanised by overdose of barbiturate or cervical dislocation, and livers and caeca removed for Campylobacte spp. assessment. 5. All liver and 28/30 caeca samples tested positive for Campylobacte spp., with mix A and C giving higher counts in the caeca than mix B. The method of euthanasia did not affect Campylobacter spp. counts. 6. In conclusion, a successful method for reliably colonising broiler chickens with Campylobacter spp. has been developed which negates the need for gavaging and is more representative of how contamination occurs in the field.

AB - 1. Chicken-associated Campylobacter spp. are the cause of most food poisoning cases in Europe. In order to study the host-pathogen interactions, a reliable and reproducible method of colonising chickens with the bacteria is required. 2. This study aimed to identify a more appropriate and less invasive method of colonisation (cf. gavaging) by seeding bedding material (litter) that commercial chickens are kept on with a mixture of Campylobacter spp., broth and faeces. 3. The first phase of the study tested the longevity of Campylobacter spp. recovery in seeded litter over 24 h: significantly more Campylobacter spp. was recovered at 0 or 3 h post-seeding than at 6 and 24 h post-seeding, indicating that the pathogen can survive to detectable levels for at least 3 h in this environment. 4. In the second phase, three groups of 10 broiler chickens (negative for Campylobacter spp. prior to exposure) were exposed at 21 days of age to one of three different Campylobacter jejuni and C. coli mixes (A, B, C), using the method above. At 28 days of age, birds were euthanised by overdose of barbiturate or cervical dislocation, and livers and caeca removed for Campylobacte spp. assessment. 5. All liver and 28/30 caeca samples tested positive for Campylobacte spp., with mix A and C giving higher counts in the caeca than mix B. The method of euthanasia did not affect Campylobacter spp. counts. 6. In conclusion, a successful method for reliably colonising broiler chickens with Campylobacter spp. has been developed which negates the need for gavaging and is more representative of how contamination occurs in the field.

KW - Caeca

KW - Contamination

KW - Food-borne illness

KW - Gavage

KW - Liver

KW - Seeding

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DO - 10.1080/00071668.2018.1523538

M3 - Article

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JO - British Poultry Science

JF - British Poultry Science

SN - 0007-1668

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ER -