Restricted dog leucocyte antigen (DLA) class II haplotypes and genotypes in Beagles

Francesca Soutter, Lorna J Kennedy, William E R Ollier, Laia Solano-Gallego, Brian Catchpole

Research output: Contribution to journalShort communication peer-review

5 Citations (Scopus)

Abstract

Beagles are commonly used in vaccine trials as part of the regulatory approval process. Genetic restriction within this breed and the impact this might have on vaccine responses are rarely considered. This study was designed to characterise diversity of dog leucocyte antigen (DLA) class II genes in a breeding colony of laboratory Beagles, whose offspring are used in vaccine studies. DLA haplotypes were determined by PCR and sequence-based typing from genomic DNA extracted from blood. Breeding colony Beagles had significantly different DLA haplotype frequencies in comparison with pet Beagles and both groups showed limited DLA diversity. Restricted DLA class II genetic variability within Beagles might result in selective antigen presentation and vaccine responses that are not necessarily representative of those seen in other dog breeds.

Original languageEnglish
Pages (from-to)345-7
Number of pages3
JournalVeterinary Journal
Volume203
Issue number3
DOIs
Publication statusPrint publication - Mar 2015
Externally publishedYes

Keywords

  • Animals
  • Dogs/genetics
  • Genotype
  • Haplotypes
  • Histocompatibility Antigens/genetics
  • Species Specificity

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