Rumen bacterial community responses to DPA, EPA and DHA in cattle and sheep: a comparative in vitro study

D Carreño, P G Toral, E Pinloche, A Belenguer, D R Yáñez-Ruiz, G Hervás, N R McEwan, C J Newbold, P Frutos

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Abstract

The role of marine lipids as modulators of ruminal biohydrogenation of dietary unsaturated fatty acids may be explained by the effects of their n-3 polyunsaturated fatty acids (PUFA) on the bacterial community. However, the impact of individual PUFA has barely been examined, and it is uncertain which bacteria are truly involved in biohydrogenation. In addition, despite interspecies differences in rumen bacterial composition, we are not aware of any direct comparison of bovine and ovine responses to dietary PUFA. Therefore, rumen fluid from cannulated cattle and sheep were used as inocula to examine in vitro the effect of 20:5n-3 (EPA), 22:5n-3 (DPA), and 22:6n-3 (DHA) on the bacterial community. Amplicon 16 S rRNA sequencing suggested that EPA and DHA had a greater contribution to the action of marine lipids than DPA both in cattle and sheep. Certain effects were exclusive to each ruminant species, which underlines the complexity of rumen microbial responses to dietary fatty acids. Based on changes in bacterial abundance, Barnesiella, Prevotella, Paraprevotella, Hallela, Anaerovorax, Succiniclasticum, Ruminococcus and Ruminobacter may be involved in the ruminal response in biohydrogenation to the addition of marine lipids, but further research is necessary to confirm their actual role in ruminal lipid metabolism.

Original languageEnglish
Article number11857
JournalScientific Reports
Volume9
Issue number1
Early online date14 Aug 2019
DOIs
Publication statusFirst published - 14 Aug 2019

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Rumen
Unsaturated Fatty Acids
Sheep
Lipids
Ruminococcus
Prevotella
Omega-3 Fatty Acids
Ruminants
Lipid Metabolism
Fatty Acids
Bacteria
Research
In Vitro Techniques

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Carreño, D., Toral, P. G., Pinloche, E., Belenguer, A., Yáñez-Ruiz, D. R., Hervás, G., ... Frutos, P. (2019). Rumen bacterial community responses to DPA, EPA and DHA in cattle and sheep: a comparative in vitro study. Scientific Reports, 9(1), [11857]. https://doi.org/10.1038/s41598-019-48294-y
Carreño, D ; Toral, P G ; Pinloche, E ; Belenguer, A ; Yáñez-Ruiz, D R ; Hervás, G ; McEwan, N R ; Newbold, C J ; Frutos, P. / Rumen bacterial community responses to DPA, EPA and DHA in cattle and sheep: a comparative in vitro study. In: Scientific Reports. 2019 ; Vol. 9, No. 1.
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Carreño, D, Toral, PG, Pinloche, E, Belenguer, A, Yáñez-Ruiz, DR, Hervás, G, McEwan, NR, Newbold, CJ & Frutos, P 2019, 'Rumen bacterial community responses to DPA, EPA and DHA in cattle and sheep: a comparative in vitro study', Scientific Reports, vol. 9, no. 1, 11857. https://doi.org/10.1038/s41598-019-48294-y

Rumen bacterial community responses to DPA, EPA and DHA in cattle and sheep: a comparative in vitro study. / Carreño, D; Toral, P G; Pinloche, E; Belenguer, A; Yáñez-Ruiz, D R; Hervás, G; McEwan, N R; Newbold, C J; Frutos, P.

In: Scientific Reports, Vol. 9, No. 1, 11857, 14.08.2019.

Research output: Contribution to journalArticle

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Carreño D, Toral PG, Pinloche E, Belenguer A, Yáñez-Ruiz DR, Hervás G et al. Rumen bacterial community responses to DPA, EPA and DHA in cattle and sheep: a comparative in vitro study. Scientific Reports. 2019 Aug 14;9(1). 11857. https://doi.org/10.1038/s41598-019-48294-y