Rumen microbial population dynamics in response to photoperiod

N R McEwan, L Abecia, M Regensbogenova, C L Adam, P A Findlay, C J Newbold

Research output: Contribution to journalArticleResearchpeer-review

28 Citations (Scopus)

Abstract

AIMS: This work was carried out to determine if there was a difference in the microbial population of the rumen associated with daylength at which sheep are housed.

METHODS AND RESULTS: Denaturing gradient gel electrophoresis (DGGE) was used to study the ciliate and bacterial diversity in the rumen of Soay rams kept in long day (16 h light) or short day (8 h light) photoperiods. Bacterial diversity varied according to the daylength conditions where the host animal was housed, as did total volatile fatty acids (VFA) concentrations. No differences associated with daylength were detected in ciliate diversity, branched VFA concentrations or the ruminal ammonia concentrations.

CONCLUSIONS: As diets had identical composition, yet voluntary intakes levels were higher during long days, it is proposed that the differences in bacterial populations arise because of the differences in amount of food consumed.

SIGNIFICANCE AND IMPACT OF THE STUDY: The outcome of this study demonstrated that factors beyond dietary composition must be taken into account when trying to study microbial populations, even in what can be considered a fairly constant environment.

Original languageEnglish
Pages (from-to)97-101
Number of pages5
JournalLetters in Applied Microbiology
Volume41
Issue number1
DOIs
Publication statusPrint publication - 2005
Externally publishedYes

Fingerprint

Rumen
Photoperiod
Population Dynamics
Volatile Fatty Acids
Population
Light
Denaturing Gradient Gel Electrophoresis
Ammonia
Sheep
Outcome Assessment (Health Care)
Diet
Food

Keywords

  • Animals
  • Bacteria/genetics
  • DNA Fingerprinting
  • DNA, Bacterial/analysis
  • Eating/physiology
  • Electrophoresis, Polyacrylamide Gel
  • Photoperiod
  • Rumen/chemistry
  • Sheep/microbiology
  • Time Factors

Cite this

McEwan, N R ; Abecia, L ; Regensbogenova, M ; Adam, C L ; Findlay, P A ; Newbold, C J. / Rumen microbial population dynamics in response to photoperiod. In: Letters in Applied Microbiology. 2005 ; Vol. 41, No. 1. pp. 97-101.
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abstract = "AIMS: This work was carried out to determine if there was a difference in the microbial population of the rumen associated with daylength at which sheep are housed.METHODS AND RESULTS: Denaturing gradient gel electrophoresis (DGGE) was used to study the ciliate and bacterial diversity in the rumen of Soay rams kept in long day (16 h light) or short day (8 h light) photoperiods. Bacterial diversity varied according to the daylength conditions where the host animal was housed, as did total volatile fatty acids (VFA) concentrations. No differences associated with daylength were detected in ciliate diversity, branched VFA concentrations or the ruminal ammonia concentrations.CONCLUSIONS: As diets had identical composition, yet voluntary intakes levels were higher during long days, it is proposed that the differences in bacterial populations arise because of the differences in amount of food consumed.SIGNIFICANCE AND IMPACT OF THE STUDY: The outcome of this study demonstrated that factors beyond dietary composition must be taken into account when trying to study microbial populations, even in what can be considered a fairly constant environment.",
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McEwan, NR, Abecia, L, Regensbogenova, M, Adam, CL, Findlay, PA & Newbold, CJ 2005, 'Rumen microbial population dynamics in response to photoperiod', Letters in Applied Microbiology, vol. 41, no. 1, pp. 97-101. https://doi.org/10.1111/j.1472-765X.2005.01707.x

Rumen microbial population dynamics in response to photoperiod. / McEwan, N R; Abecia, L; Regensbogenova, M; Adam, C L; Findlay, P A; Newbold, C J.

In: Letters in Applied Microbiology, Vol. 41, No. 1, 2005, p. 97-101.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Rumen microbial population dynamics in response to photoperiod

AU - McEwan, N R

AU - Abecia, L

AU - Regensbogenova, M

AU - Adam, C L

AU - Findlay, P A

AU - Newbold, C J

PY - 2005

Y1 - 2005

N2 - AIMS: This work was carried out to determine if there was a difference in the microbial population of the rumen associated with daylength at which sheep are housed.METHODS AND RESULTS: Denaturing gradient gel electrophoresis (DGGE) was used to study the ciliate and bacterial diversity in the rumen of Soay rams kept in long day (16 h light) or short day (8 h light) photoperiods. Bacterial diversity varied according to the daylength conditions where the host animal was housed, as did total volatile fatty acids (VFA) concentrations. No differences associated with daylength were detected in ciliate diversity, branched VFA concentrations or the ruminal ammonia concentrations.CONCLUSIONS: As diets had identical composition, yet voluntary intakes levels were higher during long days, it is proposed that the differences in bacterial populations arise because of the differences in amount of food consumed.SIGNIFICANCE AND IMPACT OF THE STUDY: The outcome of this study demonstrated that factors beyond dietary composition must be taken into account when trying to study microbial populations, even in what can be considered a fairly constant environment.

AB - AIMS: This work was carried out to determine if there was a difference in the microbial population of the rumen associated with daylength at which sheep are housed.METHODS AND RESULTS: Denaturing gradient gel electrophoresis (DGGE) was used to study the ciliate and bacterial diversity in the rumen of Soay rams kept in long day (16 h light) or short day (8 h light) photoperiods. Bacterial diversity varied according to the daylength conditions where the host animal was housed, as did total volatile fatty acids (VFA) concentrations. No differences associated with daylength were detected in ciliate diversity, branched VFA concentrations or the ruminal ammonia concentrations.CONCLUSIONS: As diets had identical composition, yet voluntary intakes levels were higher during long days, it is proposed that the differences in bacterial populations arise because of the differences in amount of food consumed.SIGNIFICANCE AND IMPACT OF THE STUDY: The outcome of this study demonstrated that factors beyond dietary composition must be taken into account when trying to study microbial populations, even in what can be considered a fairly constant environment.

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KW - DNA, Bacterial/analysis

KW - Eating/physiology

KW - Electrophoresis, Polyacrylamide Gel

KW - Photoperiod

KW - Rumen/chemistry

KW - Sheep/microbiology

KW - Time Factors

U2 - 10.1111/j.1472-765X.2005.01707.x

DO - 10.1111/j.1472-765X.2005.01707.x

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JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

SN - 0266-8254

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