Rumen protozoa are rich in polyunsaturated fatty acids due to the ingestion of chloroplasts

Sharon A Huws, Eun J Kim, Alison H Kingston-Smith, Michael R F Lee, Stefan M Muetzel, Alan R Cookson, Charles J Newbold, R John Wallace, Nigel D Scollan

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

Within this study, we investigated whether the polyunsaturated fatty acids (PUFA)-rich nature of rumen protozoa is a consequence of ingestion of PUFA-rich chloroplasts. Four Hereford x Friesian steers were offered hay [low 18:3 (n-3) and low chlorophyll concentration] followed by freshly cut perennial ryegrass [high 18:3 (n-3) and high chlorophyll concentration] for 16 days. On the 14th and 16th days, rumen protozoa as well as attached and planktonic bacteria were fractionated 1 h before (-1 h), 2 and 6 h postfeeding, and their fatty acid concentrations determined. Protozoa fractionated from fresh grass-fed steers were richer (P<0.05) in PUFA, except conjugated linoleic acid, for all time points compared with those from hay-fed steers. Protozoal density was higher (P<0.05) for grass compared with hay. Entodinomorphid abundance was 3.4 times higher on fresh grass (P<0.01) compared with hay. Confocal microscopy and transmission electron microscopy confirmed that Epidinium spp. were commonly saturated with intracellular cytoplasmic chloroplasts. These data suggest that engulfment of chloroplasts is a major contributor to the high 18:3 (n-3) concentration of protozoa.

Original languageEnglish
Pages (from-to)461-71
Number of pages11
JournalFEMS Microbiology Ecology
Volume69
Issue number3
DOIs
Publication statusPrint publication - Sep 2009
Externally publishedYes

Keywords

  • Animal Feed/analysis
  • Animals
  • Bacteria/genetics
  • Cattle
  • Chlorophyll/metabolism
  • Chloroplasts/metabolism
  • DNA, Bacterial/genetics
  • DNA, Protozoan/genetics
  • Eukaryota/genetics
  • Fatty Acids, Unsaturated/metabolism
  • Male
  • RNA, Ribosomal, 16S/genetics
  • RNA, Ribosomal, 18S/genetics
  • Rumen/microbiology

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