Secretome profiling reveals virulence-associated proteins of Fusarium proliferatum during interaction with banana fruit

Taotao Li, Yu Wu, Yong Wang, Haiyan Gao, Vijai Kumar Gupta*, Xuewu Duan, Hongxia Qu, Yueming Jiang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Secreted proteins are vital for the pathogenicity of many fungi through manipulating their hosts for efficient colonization. Fusarium proliferatum is a phytopathogenic fungus infecting many crops, vegetables, and fruit, including banana fruit. To access the proteins involved in pathogen–host interaction, we used label-free quantitative proteomics technology to comparatively analyze the secretomes of F. proliferatum cultured with and without banana peel in Czapek’s broth medium. By analyzing the secretomes of F. proliferatum, we have identified 105 proteins with 40 exclusively secreted and 65 increased in abundance in response to a banana peel. These proteins were involved in the promotion of invasion of banana fruit, and they were mainly categorized into virulence factors, cell wall degradation, metabolic process, response to stress, regulation, and another unknown biological process. The expressions of corresponding genes confirmed the existence of these secreted proteins in the banana peel. Furthermore, expression pattern suggested variable roles for these genes at different infection stages. This study expanded the current database of F. proliferatum secreted proteins which might be involved in the infection strategy of this fungus. Additionally, this study warranted the further attention of some secreted proteins that might initiate infection of F. proliferatum on banana fruit.

Original languageEnglish
Article number246
JournalBiomolecules
Volume9
Issue number6
Early online date23 Jun 2019
DOIs
Publication statusFirst published - 23 Jun 2019
Externally publishedYes

Keywords

  • Fungi
  • Gene expression
  • Pathogenicity
  • Plant host
  • Secreted proteins
  • Virulence

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