Sequence polymorphism in polyploid wheat and their d-genome diploid ancestor

Katherine S Caldwell, Jan Dvorak, Evans S Lagudah, Eduard Akhunov, Ming-Cheng Luo, Petra Wolters, Wayne Powell

Research output: Contribution to journalArticleResearchpeer-review

114 Citations (Scopus)

Abstract

Sequencing was used to investigate the origin of the D genome of the allopolyploid species Triticum aestivum and Aegilops cylindrica. A 247-bp region of the wheat D-genome Xwye838 locus, encoding ADP-glucopyrophosphorylase, and a 326-bp region of the wheat D-genome Gss locus, encoding granule-bound starch synthase, were sequenced in a total 564 lines of hexaploid wheat (T. aestivum, genome AABBDD) involving all its subspecies and 203 lines of Aegilops tauschii, the diploid source of the wheat D genome. In Ae. tauschii, two SNP variants were detected at the Xwye838 locus and 11 haplotypes at the Gss locus. Two haplotypes with contrasting frequencies were found at each locus in wheat. Both wheat Xwye838 variants, but only one of the Gss haplotypes seen in wheat, were found among the Ae. tauschii lines. The other wheat Gss haplotype was not found in either Ae. tauschii or 70 lines of tetraploid Ae. cylindrica (genomes CCDD), which is known to hybridize with wheat. It is concluded that both T. aestivum and Ae. cylindrica originated recurrently, with at least two genetically distinct progenitors contributing to the formation of the D genome in both species.

Original languageEnglish
Pages (from-to)941-7
Number of pages7
JournalGenetics
Volume167
Issue number2
DOIs
Publication statusPrint publication - Jun 2004
Externally publishedYes

Fingerprint

Polyploidy
Diploidy
Triticum
Genome
Haplotypes
Starch Synthase
Tetraploidy
Adenosine Diphosphate
Single Nucleotide Polymorphism

Keywords

  • Base Sequence
  • DNA Primers
  • Diploidy
  • Genetic Variation
  • Genome, Plant
  • Nucleic Acid Conformation
  • Polymerase Chain Reaction/methods
  • Polyploidy
  • Sequence Alignment
  • Triticum/classification

Cite this

Caldwell, K. S., Dvorak, J., Lagudah, E. S., Akhunov, E., Luo, M-C., Wolters, P., & Powell, W. (2004). Sequence polymorphism in polyploid wheat and their d-genome diploid ancestor. Genetics, 167(2), 941-7. https://doi.org/10.1534/genetics.103.016303
Caldwell, Katherine S ; Dvorak, Jan ; Lagudah, Evans S ; Akhunov, Eduard ; Luo, Ming-Cheng ; Wolters, Petra ; Powell, Wayne. / Sequence polymorphism in polyploid wheat and their d-genome diploid ancestor. In: Genetics. 2004 ; Vol. 167, No. 2. pp. 941-7.
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Caldwell, KS, Dvorak, J, Lagudah, ES, Akhunov, E, Luo, M-C, Wolters, P & Powell, W 2004, 'Sequence polymorphism in polyploid wheat and their d-genome diploid ancestor', Genetics, vol. 167, no. 2, pp. 941-7. https://doi.org/10.1534/genetics.103.016303

Sequence polymorphism in polyploid wheat and their d-genome diploid ancestor. / Caldwell, Katherine S; Dvorak, Jan; Lagudah, Evans S; Akhunov, Eduard; Luo, Ming-Cheng; Wolters, Petra; Powell, Wayne.

In: Genetics, Vol. 167, No. 2, 06.2004, p. 941-7.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Sequence polymorphism in polyploid wheat and their d-genome diploid ancestor

AU - Caldwell, Katherine S

AU - Dvorak, Jan

AU - Lagudah, Evans S

AU - Akhunov, Eduard

AU - Luo, Ming-Cheng

AU - Wolters, Petra

AU - Powell, Wayne

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N2 - Sequencing was used to investigate the origin of the D genome of the allopolyploid species Triticum aestivum and Aegilops cylindrica. A 247-bp region of the wheat D-genome Xwye838 locus, encoding ADP-glucopyrophosphorylase, and a 326-bp region of the wheat D-genome Gss locus, encoding granule-bound starch synthase, were sequenced in a total 564 lines of hexaploid wheat (T. aestivum, genome AABBDD) involving all its subspecies and 203 lines of Aegilops tauschii, the diploid source of the wheat D genome. In Ae. tauschii, two SNP variants were detected at the Xwye838 locus and 11 haplotypes at the Gss locus. Two haplotypes with contrasting frequencies were found at each locus in wheat. Both wheat Xwye838 variants, but only one of the Gss haplotypes seen in wheat, were found among the Ae. tauschii lines. The other wheat Gss haplotype was not found in either Ae. tauschii or 70 lines of tetraploid Ae. cylindrica (genomes CCDD), which is known to hybridize with wheat. It is concluded that both T. aestivum and Ae. cylindrica originated recurrently, with at least two genetically distinct progenitors contributing to the formation of the D genome in both species.

AB - Sequencing was used to investigate the origin of the D genome of the allopolyploid species Triticum aestivum and Aegilops cylindrica. A 247-bp region of the wheat D-genome Xwye838 locus, encoding ADP-glucopyrophosphorylase, and a 326-bp region of the wheat D-genome Gss locus, encoding granule-bound starch synthase, were sequenced in a total 564 lines of hexaploid wheat (T. aestivum, genome AABBDD) involving all its subspecies and 203 lines of Aegilops tauschii, the diploid source of the wheat D genome. In Ae. tauschii, two SNP variants were detected at the Xwye838 locus and 11 haplotypes at the Gss locus. Two haplotypes with contrasting frequencies were found at each locus in wheat. Both wheat Xwye838 variants, but only one of the Gss haplotypes seen in wheat, were found among the Ae. tauschii lines. The other wheat Gss haplotype was not found in either Ae. tauschii or 70 lines of tetraploid Ae. cylindrica (genomes CCDD), which is known to hybridize with wheat. It is concluded that both T. aestivum and Ae. cylindrica originated recurrently, with at least two genetically distinct progenitors contributing to the formation of the D genome in both species.

KW - Base Sequence

KW - DNA Primers

KW - Diploidy

KW - Genetic Variation

KW - Genome, Plant

KW - Nucleic Acid Conformation

KW - Polymerase Chain Reaction/methods

KW - Polyploidy

KW - Sequence Alignment

KW - Triticum/classification

U2 - 10.1534/genetics.103.016303

DO - 10.1534/genetics.103.016303

M3 - Article

VL - 167

SP - 941

EP - 947

JO - Genetics

JF - Genetics

SN - 0016-6731

IS - 2

ER -

Caldwell KS, Dvorak J, Lagudah ES, Akhunov E, Luo M-C, Wolters P et al. Sequence polymorphism in polyploid wheat and their d-genome diploid ancestor. Genetics. 2004 Jun;167(2):941-7. https://doi.org/10.1534/genetics.103.016303