Short communication: Identification of variation in the ovine prolactin gene of Chios sheep with a cost effective sequence based typing assay

D Miltiadou, M Orford, S Symeou, G Banos

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3 Citations (Scopus)
3 Downloads (Pure)

Abstract

The present study identified single nucleotide polymorphisms (SNP) in the coding and untranslated regions of the ovine prolactin gene of Chios sheep. By developing a cost-effective direct sequence-based typing assay, around 600 bp of reliable sequencing data and clear identification of heterozygous positions was achieved. Five SNP were found, located in exons 2 (KC764410:g.567G > A, g.625C > T, g.683C > A) and 3 (KC764410:g.2015C > A, g.2101G > A), whereas the remaining exons were monomorphic. The identified SNP were synonymous, with the exception of the g.567G > A SNP, which results in an Arg to His amino acid change. As the sequencing cost of the sequence-based typing assay was 20 orders of magnitude lower compared with a standard Sanger method, the assay was also used as a genotyping tool. The identified polymorphism was genotyped for 247 ewes and was subsequently used in mixed model association analyses of milk yield, milk fat content, and litter size at birth. The association analysis revealed a significant dominance effect of 0.17 ± 0.07 of the g.2015C > A SNP on milk fat percentage, whereas a dominance effect of −21.33 ± 10.51 of the same SNP on total lactation milk yield was also estimated. The g.2015C > A SNP explained 2.47 and 3.68% of the total phenotypic variance of milk yield and milk fat percentage, respectively, whereas the corresponding values for the animal variance were 7.14 and 11.75%. A suggestive association of the nonsynonymous g.567G > A SNP with litter size at birth was also detected
Original languageEnglish
Pages (from-to)1290 - 1294
Number of pages5
JournalJournal of Dairy Science
Volume100
Issue number2
Early online date21 Dec 2016
DOIs
Publication statusFirst published - 21 Dec 2016

Fingerprint

Chios (sheep breed)
prolactin
animal communication
single nucleotide polymorphism
sheep
assays
genes
milk yield
milk fat percentage
litter size
dominance (genetics)
exons
phenotypic variation
milk fat
genotyping
ewes
lactation
lipid content
genetic polymorphism

Bibliographical note

1030786

Keywords

  • Prolactin association
  • SNP identification
  • Sequence based typing (SBT)
  • Sheep

Cite this

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abstract = "The present study identified single nucleotide polymorphisms (SNP) in the coding and untranslated regions of the ovine prolactin gene of Chios sheep. By developing a cost-effective direct sequence-based typing assay, around 600 bp of reliable sequencing data and clear identification of heterozygous positions was achieved. Five SNP were found, located in exons 2 (KC764410:g.567G > A, g.625C > T, g.683C > A) and 3 (KC764410:g.2015C > A, g.2101G > A), whereas the remaining exons were monomorphic. The identified SNP were synonymous, with the exception of the g.567G > A SNP, which results in an Arg to His amino acid change. As the sequencing cost of the sequence-based typing assay was 20 orders of magnitude lower compared with a standard Sanger method, the assay was also used as a genotyping tool. The identified polymorphism was genotyped for 247 ewes and was subsequently used in mixed model association analyses of milk yield, milk fat content, and litter size at birth. The association analysis revealed a significant dominance effect of 0.17 ± 0.07 of the g.2015C > A SNP on milk fat percentage, whereas a dominance effect of −21.33 ± 10.51 of the same SNP on total lactation milk yield was also estimated. The g.2015C > A SNP explained 2.47 and 3.68{\%} of the total phenotypic variance of milk yield and milk fat percentage, respectively, whereas the corresponding values for the animal variance were 7.14 and 11.75{\%}. A suggestive association of the nonsynonymous g.567G > A SNP with litter size at birth was also detected",
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Short communication: Identification of variation in the ovine prolactin gene of Chios sheep with a cost effective sequence based typing assay. / Miltiadou, D; Orford, M; Symeou, S; Banos, G.

In: Journal of Dairy Science, Vol. 100, No. 2, 21.12.2016, p. 1290 - 1294.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Short communication: Identification of variation in the ovine prolactin gene of Chios sheep with a cost effective sequence based typing assay

AU - Miltiadou, D

AU - Orford, M

AU - Symeou, S

AU - Banos, G

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AB - The present study identified single nucleotide polymorphisms (SNP) in the coding and untranslated regions of the ovine prolactin gene of Chios sheep. By developing a cost-effective direct sequence-based typing assay, around 600 bp of reliable sequencing data and clear identification of heterozygous positions was achieved. Five SNP were found, located in exons 2 (KC764410:g.567G > A, g.625C > T, g.683C > A) and 3 (KC764410:g.2015C > A, g.2101G > A), whereas the remaining exons were monomorphic. The identified SNP were synonymous, with the exception of the g.567G > A SNP, which results in an Arg to His amino acid change. As the sequencing cost of the sequence-based typing assay was 20 orders of magnitude lower compared with a standard Sanger method, the assay was also used as a genotyping tool. The identified polymorphism was genotyped for 247 ewes and was subsequently used in mixed model association analyses of milk yield, milk fat content, and litter size at birth. The association analysis revealed a significant dominance effect of 0.17 ± 0.07 of the g.2015C > A SNP on milk fat percentage, whereas a dominance effect of −21.33 ± 10.51 of the same SNP on total lactation milk yield was also estimated. The g.2015C > A SNP explained 2.47 and 3.68% of the total phenotypic variance of milk yield and milk fat percentage, respectively, whereas the corresponding values for the animal variance were 7.14 and 11.75%. A suggestive association of the nonsynonymous g.567G > A SNP with litter size at birth was also detected

KW - Prolactin association

KW - SNP identification

KW - Sequence based typing (SBT)

KW - Sheep

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DO - 10.3168/jds.2016-11721

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JO - Journal of Dairy Science

JF - Journal of Dairy Science

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