Simultaneous detection and quantification of six equine cytokines in plasma using a fluorescent microsphere immunoassay (FMIA)

SA Hall; D Stucke; B Morrone; D Lebelt; AJ Zanella

doi:10.1016/j.mex.2015.04.002

Simultaneous detection and quantification of six equine cytokines in plasma using a fluorescent microsphere immunoassay (FMIA)

SA Hall, D Stucke, B Morrone, D Lebelt, AJ Zanella

Animal Welfare

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Abstract

Cytokines are cell signalling proteins that mediate a number of different physiological responses. They are also biomarkers for inflammatory conditions and potential diagnostic references for diseases. Until recently, simultaneous quantification of cytokine profiles had not been possible. Now however, fluorescent microsphere immunoassays (FMIA) are able to measure multiple cytokines in a single sample. The following pro-inflammatory and anti inflammatory cytokines were quantified in equine plasma and serum samples: Interleukin (IL)-2, IL-4, IL-6, IL-10, Interferon (IFN)-γ, Tumor necrosis factor (TNF)-α.

Original language	English
Pages (from-to)	241 - 248
Number of pages	8
Journal	MethodsX
Volume	2
DOIs	https://doi.org/10.1016/j.mex.2015.04.002
Publication status	First published - 2015

Bibliographical note

1023306

Keywords

Cytokine profile
Equine plasma
FMIA
Multiplex
Six-plex

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10.1016/j.mex.2015.04.002

14863975.pdfFinal published version, 6.16 MBLicence: CC BY

Biomarkers Laboratory
Lowe, A. (Manager)
Veterinary And Animal Sciences
Facility/equipment: Facility

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T1 - Simultaneous detection and quantification of six equine cytokines in plasma using a fluorescent microsphere immunoassay (FMIA)

AU - Hall, SA

AU - Stucke, D

AU - Morrone, B

AU - Lebelt, D

AU - Zanella, AJ

N1 - 1023306

PY - 2015

Y1 - 2015

N2 - Cytokines are cell signalling proteins that mediate a number of different physiological responses. They are also biomarkers for inflammatory conditions and potential diagnostic references for diseases. Until recently, simultaneous quantification of cytokine profiles had not been possible. Now however, fluorescent microsphere immunoassays (FMIA) are able to measure multiple cytokines in a single sample. The following pro-inflammatory and anti inflammatory cytokines were quantified in equine plasma and serum samples: Interleukin (IL)-2, IL-4, IL-6, IL-10, Interferon (IFN)-γ, Tumor necrosis factor (TNF)-α.

AB - Cytokines are cell signalling proteins that mediate a number of different physiological responses. They are also biomarkers for inflammatory conditions and potential diagnostic references for diseases. Until recently, simultaneous quantification of cytokine profiles had not been possible. Now however, fluorescent microsphere immunoassays (FMIA) are able to measure multiple cytokines in a single sample. The following pro-inflammatory and anti inflammatory cytokines were quantified in equine plasma and serum samples: Interleukin (IL)-2, IL-4, IL-6, IL-10, Interferon (IFN)-γ, Tumor necrosis factor (TNF)-α.

KW - Cytokine profile

KW - Equine plasma

KW - FMIA

KW - Multiplex

KW - Six-plex

U2 - 10.1016/j.mex.2015.04.002

DO - 10.1016/j.mex.2015.04.002

M3 - Article

SN - 2215-0161

VL - 2

SP - 241

EP - 248

JO - MethodsX

JF - MethodsX

ER -

Simultaneous detection and quantification of six equine cytokines in plasma using a fluorescent microsphere immunoassay (FMIA)

Abstract

Bibliographical note

Keywords

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Biomarkers Laboratory

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