Simultaneous detection and quantification of six equine cytokines in plasma using a fluorescent microsphere immunoassay (FMIA)

SA Hall, D Stucke, B Morrone, D Lebelt, AJ Zanella

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Cytokines are cell signalling proteins that mediate a number of different physiological responses. They are also biomarkers for inflammatory conditions and potential diagnostic references for diseases. Until recently, simultaneous quantification of cytokine profiles had not been possible. Now however, fluorescent microsphere immunoassays (FMIA) are able to measure multiple cytokines in a single sample. The following pro-inflammatory and anti inflammatory cytokines were quantified in equine plasma and serum samples: Interleukin (IL)-2, IL-4, IL-6, IL-10, Interferon (IFN)-γ, Tumor necrosis factor (TNF)-α.
Original languageEnglish
Pages (from-to)241 - 248
Number of pages8
JournalMethodsX
Volume2
DOIs
Publication statusFirst published - 2015

Fingerprint

Microspheres
Cytokines
Plasmas
Cell signaling
Biomarkers
Interleukin-4
Interleukin-10
Interferons
Interleukin-2
Interleukin-6
Anti-Inflammatory Agents
Tumor Necrosis Factor-alpha
Proteins

Bibliographical note

1023306

Keywords

  • Cytokine profile
  • Equine plasma
  • FMIA
  • Multiplex
  • Six-plex

Cite this

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Simultaneous detection and quantification of six equine cytokines in plasma using a fluorescent microsphere immunoassay (FMIA). / Hall, SA; Stucke, D; Morrone, B; Lebelt, D; Zanella, AJ.

In: MethodsX, Vol. 2, 2015, p. 241 - 248.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Simultaneous detection and quantification of six equine cytokines in plasma using a fluorescent microsphere immunoassay (FMIA)

AU - Hall, SA

AU - Stucke, D

AU - Morrone, B

AU - Lebelt, D

AU - Zanella, AJ

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PY - 2015

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AB - Cytokines are cell signalling proteins that mediate a number of different physiological responses. They are also biomarkers for inflammatory conditions and potential diagnostic references for diseases. Until recently, simultaneous quantification of cytokine profiles had not been possible. Now however, fluorescent microsphere immunoassays (FMIA) are able to measure multiple cytokines in a single sample. The following pro-inflammatory and anti inflammatory cytokines were quantified in equine plasma and serum samples: Interleukin (IL)-2, IL-4, IL-6, IL-10, Interferon (IFN)-γ, Tumor necrosis factor (TNF)-α.

KW - Cytokine profile

KW - Equine plasma

KW - FMIA

KW - Multiplex

KW - Six-plex

U2 - 10.1016/j.mex.2015.04.002

DO - 10.1016/j.mex.2015.04.002

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