Skeletal muscle cation contents and effects of genetic selection for production traits: a relationship with spontaneous myopathy?

DA Sandercock*, MA Mitchell, Zoe E Barker

*Corresponding author for this work

Research output: Contribution to journalMeeting Abstract

Abstract

Recent studies have indicated that genetic selection for useful production traits in meat type poultry is associated with an increased incidence and degree of idiopathic (spontaneous) and stress-induced myopathies. The mechanisms mediating these pathologies await full elucidation but alterations in intracellular calcium homeostasis have been strongly implicated. Whilst a direct role for altered function in calcium regulatory systems is well recognized, disruptions in the balance of the intracellular active concentrations of other cations may also contribute to these conditions. The present study examined the effects of genetic selection on muscle cation contents and related these to myopathic status. Pectoral muscle samples were prepared from 4 birds (49d) from each of 34 chicken pure-lines from three categories; broiler (B), traditional (T) and layers (L) selected for the relevant production traits. The contents of sodium (Na), potassium (K), calcium (Ca) and magnesium (Mg) were determined by atomic absorption spectrophotometry in muscle homogenates and expressed per unit tissue dry and ash weights. Plasma cation contents were also measured in each bird and the degree of spontaneous myopathy was assessed by plasma creatine kinase (CK) activity. Plasma Ca was 40% (p<0.001) higher in B lines whilst Mg was lower in traditional breeds. Plasma Na and K were higher in B lines than in the other categories (p<0.05). Muscle Ca content did not differ between categories. Muscle Mg content was higher in B lines than in the other lines. Dry and ash weight elemental analysis, however, revealed a 30% (p<0.01) elevation muscle Na content in B lines and a corresponding 14% increase in muscle K. These observations are consistent with myopathic and dystrophic conditions in other species. It is proposed that selection associated alterations in cation (other than Ca) regulation mechanisms play a significant role in the aetiology of spontaneous myopathy in modern poultry.
Original languageEnglish
Article numberabstract 259
Pages (from-to)61
Number of pages1
JournalPoultry Science
Volume81
Issue number1
Publication statusPrint publication - 2002
Externally publishedYes
Event91st Poultry Science Association Annual Meeting 2002 - University of Delaware, Newark, United States
Duration: 11 Aug 200214 Aug 2002
https://www.poultryscience.org/abstracts.asp

Fingerprint

muscular diseases
Genetic Selection
Muscular Diseases
skeletal muscle
Cations
cations
Skeletal Muscle
Calcium
calcium
muscles
Muscles
Magnesium
magnesium
Poultry
Birds
poultry
Pectoralis Muscles
Weights and Measures
Atomic Spectrophotometry
birds

Keywords

  • Genetic selection
  • Production traits
  • Muscle mass
  • Muscle hypertrophy
  • Intracellular cation regulation
  • Idiopathic myopathy
  • Stress-induced myopathy
  • Pectoralis major
  • Elemental analysis
  • Sodium
  • Calcium
  • Potassium
  • Magnesium
  • Ash weight
  • Dry weight
  • Intracellular potassium
  • Muscle damage
  • Intracellular sodium

Cite this

@article{4ebdd195682e44bab51c4c38bf3427e8,
title = "Skeletal muscle cation contents and effects of genetic selection for production traits: a relationship with spontaneous myopathy?",
abstract = "Recent studies have indicated that genetic selection for useful production traits in meat type poultry is associated with an increased incidence and degree of idiopathic (spontaneous) and stress-induced myopathies. The mechanisms mediating these pathologies await full elucidation but alterations in intracellular calcium homeostasis have been strongly implicated. Whilst a direct role for altered function in calcium regulatory systems is well recognized, disruptions in the balance of the intracellular active concentrations of other cations may also contribute to these conditions. The present study examined the effects of genetic selection on muscle cation contents and related these to myopathic status. Pectoral muscle samples were prepared from 4 birds (49d) from each of 34 chicken pure-lines from three categories; broiler (B), traditional (T) and layers (L) selected for the relevant production traits. The contents of sodium (Na), potassium (K), calcium (Ca) and magnesium (Mg) were determined by atomic absorption spectrophotometry in muscle homogenates and expressed per unit tissue dry and ash weights. Plasma cation contents were also measured in each bird and the degree of spontaneous myopathy was assessed by plasma creatine kinase (CK) activity. Plasma Ca was 40{\%} (p<0.001) higher in B lines whilst Mg was lower in traditional breeds. Plasma Na and K were higher in B lines than in the other categories (p<0.05). Muscle Ca content did not differ between categories. Muscle Mg content was higher in B lines than in the other lines. Dry and ash weight elemental analysis, however, revealed a 30{\%} (p<0.01) elevation muscle Na content in B lines and a corresponding 14{\%} increase in muscle K. These observations are consistent with myopathic and dystrophic conditions in other species. It is proposed that selection associated alterations in cation (other than Ca) regulation mechanisms play a significant role in the aetiology of spontaneous myopathy in modern poultry.",
keywords = "Genetic selection, Production traits, Muscle mass, Muscle hypertrophy, Intracellular cation regulation, Idiopathic myopathy, Stress-induced myopathy, Pectoralis major, Elemental analysis, Sodium, Calcium, Potassium, Magnesium, Ash weight, Dry weight, Intracellular potassium, Muscle damage, Intracellular sodium",
author = "DA Sandercock and MA Mitchell and Barker, {Zoe E}",
year = "2002",
language = "English",
volume = "81",
pages = "61",
journal = "Poultry Science",
issn = "0032-5791",
publisher = "Oxford University Press",
number = "1",

}

Skeletal muscle cation contents and effects of genetic selection for production traits: a relationship with spontaneous myopathy? / Sandercock, DA; Mitchell, MA; Barker, Zoe E.

In: Poultry Science, Vol. 81, No. 1, abstract 259, 2002, p. 61.

Research output: Contribution to journalMeeting Abstract

TY - JOUR

T1 - Skeletal muscle cation contents and effects of genetic selection for production traits: a relationship with spontaneous myopathy?

AU - Sandercock, DA

AU - Mitchell, MA

AU - Barker, Zoe E

PY - 2002

Y1 - 2002

N2 - Recent studies have indicated that genetic selection for useful production traits in meat type poultry is associated with an increased incidence and degree of idiopathic (spontaneous) and stress-induced myopathies. The mechanisms mediating these pathologies await full elucidation but alterations in intracellular calcium homeostasis have been strongly implicated. Whilst a direct role for altered function in calcium regulatory systems is well recognized, disruptions in the balance of the intracellular active concentrations of other cations may also contribute to these conditions. The present study examined the effects of genetic selection on muscle cation contents and related these to myopathic status. Pectoral muscle samples were prepared from 4 birds (49d) from each of 34 chicken pure-lines from three categories; broiler (B), traditional (T) and layers (L) selected for the relevant production traits. The contents of sodium (Na), potassium (K), calcium (Ca) and magnesium (Mg) were determined by atomic absorption spectrophotometry in muscle homogenates and expressed per unit tissue dry and ash weights. Plasma cation contents were also measured in each bird and the degree of spontaneous myopathy was assessed by plasma creatine kinase (CK) activity. Plasma Ca was 40% (p<0.001) higher in B lines whilst Mg was lower in traditional breeds. Plasma Na and K were higher in B lines than in the other categories (p<0.05). Muscle Ca content did not differ between categories. Muscle Mg content was higher in B lines than in the other lines. Dry and ash weight elemental analysis, however, revealed a 30% (p<0.01) elevation muscle Na content in B lines and a corresponding 14% increase in muscle K. These observations are consistent with myopathic and dystrophic conditions in other species. It is proposed that selection associated alterations in cation (other than Ca) regulation mechanisms play a significant role in the aetiology of spontaneous myopathy in modern poultry.

AB - Recent studies have indicated that genetic selection for useful production traits in meat type poultry is associated with an increased incidence and degree of idiopathic (spontaneous) and stress-induced myopathies. The mechanisms mediating these pathologies await full elucidation but alterations in intracellular calcium homeostasis have been strongly implicated. Whilst a direct role for altered function in calcium regulatory systems is well recognized, disruptions in the balance of the intracellular active concentrations of other cations may also contribute to these conditions. The present study examined the effects of genetic selection on muscle cation contents and related these to myopathic status. Pectoral muscle samples were prepared from 4 birds (49d) from each of 34 chicken pure-lines from three categories; broiler (B), traditional (T) and layers (L) selected for the relevant production traits. The contents of sodium (Na), potassium (K), calcium (Ca) and magnesium (Mg) were determined by atomic absorption spectrophotometry in muscle homogenates and expressed per unit tissue dry and ash weights. Plasma cation contents were also measured in each bird and the degree of spontaneous myopathy was assessed by plasma creatine kinase (CK) activity. Plasma Ca was 40% (p<0.001) higher in B lines whilst Mg was lower in traditional breeds. Plasma Na and K were higher in B lines than in the other categories (p<0.05). Muscle Ca content did not differ between categories. Muscle Mg content was higher in B lines than in the other lines. Dry and ash weight elemental analysis, however, revealed a 30% (p<0.01) elevation muscle Na content in B lines and a corresponding 14% increase in muscle K. These observations are consistent with myopathic and dystrophic conditions in other species. It is proposed that selection associated alterations in cation (other than Ca) regulation mechanisms play a significant role in the aetiology of spontaneous myopathy in modern poultry.

KW - Genetic selection

KW - Production traits

KW - Muscle mass

KW - Muscle hypertrophy

KW - Intracellular cation regulation

KW - Idiopathic myopathy

KW - Stress-induced myopathy

KW - Pectoralis major

KW - Elemental analysis

KW - Sodium

KW - Calcium

KW - Potassium

KW - Magnesium

KW - Ash weight

KW - Dry weight

KW - Intracellular potassium

KW - Muscle damage

KW - Intracellular sodium

M3 - Meeting Abstract

VL - 81

SP - 61

JO - Poultry Science

JF - Poultry Science

SN - 0032-5791

IS - 1

M1 - abstract 259

ER -