Strain-dependent cellular immune responses in cattle following Escherichia coli O157:H7 colonization

A Corbishley, NI Ahmad, K Hughes, MR Hutchings, SP McAteer, TK Connelley, H Brown, DL Gally, TN McNeilly

Research output: Contribution to journalArticleResearchpeer-review

17 Citations (Scopus)

Abstract

Enterohemorrhagic Escherichia coli (EHEC) O157:H7 causes hemorrhagic diarrhea and potentially fatal renal failure in humans. Ruminants are considered to be the primary reservoir for human infection. Vaccines that reduce shedding in cattle are only partially protective, and their underlying protective mechanisms are unknown. Studies investigating the response of cattle to colonization generally focus on humoral immunity, leaving the role of cellular immunity unclear. To inform future vaccine development, we studied the cellular immune responses of cattle during EHEC O157:H7 colonization. Calves were challenged either with a phage type 21/28 (PT21/28) strain possessing the Shiga toxin 2a (Stx2a) and Stx2c genes or with a PT32 strain possessing the Stx2c gene only. T-helper cell-associated transcripts at the terminal rectum were analyzed by reverse transcription-quantitative PCR (RT-qPCR). Induction of gamma interferon (IFN- ) and T-bet was observed with peak expression of both genes at 7 days in PT32-challenged calves, while upregulation was delayed, peaking at 21 days, in PT21/28-challenged calves. Cells isolated from gastrointestinal lymph nodes demonstrated antigen-specific proliferation and IFN- release in response to type III secreted proteins (T3SPs); however, responsiveness was suppressed in cells isolated from PT32-challenged calves. Lymph node cells showed increased expression of the proliferation marker Ki67 in CD4 T cells from PT21/28-challenged calves, NK cells from PT32-challenged calves, and CD8 and T cells from both PT21/28- and PT32-challenged calves following ex vivo restimulation with T3SPs. This study demonstrates that cattle mount cellular immune responses during colonization with EHEC O157: H7, the temporality of which is strain dependent, with further evidence of strain-specific immunomodulation.
Original languageEnglish
Pages (from-to)5117 - 5131
Number of pages15
JournalInfection and Immunity
Volume82
Issue number12
DOIs
Publication statusFirst published - 2014

Fingerprint

Escherichia coli O157
Enterohemorrhagic Escherichia coli
Cellular Immunity
Bacteriophages
Vaccines
Lymph Nodes
Shiga Toxin
T-Lymphocytes
Immunomodulation
Ruminants
Humoral Immunity
Helper-Inducer T-Lymphocytes
Rectum
Natural Killer Cells
Interferons
Genes
Reverse Transcription
Interferon-gamma
Renal Insufficiency
Diarrhea

Bibliographical note

1020802
1023364

Keywords

  • Cattle
  • Escherichia coli
  • Immunity

Cite this

Corbishley, A., Ahmad, NI., Hughes, K., Hutchings, MR., McAteer, SP., Connelley, TK., ... McNeilly, TN. (2014). Strain-dependent cellular immune responses in cattle following Escherichia coli O157:H7 colonization. Infection and Immunity, 82(12), 5117 - 5131. https://doi.org/10.1128/IAI.02462-14
Corbishley, A ; Ahmad, NI ; Hughes, K ; Hutchings, MR ; McAteer, SP ; Connelley, TK ; Brown, H ; Gally, DL ; McNeilly, TN. / Strain-dependent cellular immune responses in cattle following Escherichia coli O157:H7 colonization. In: Infection and Immunity. 2014 ; Vol. 82, No. 12. pp. 5117 - 5131.
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Corbishley, A, Ahmad, NI, Hughes, K, Hutchings, MR, McAteer, SP, Connelley, TK, Brown, H, Gally, DL & McNeilly, TN 2014, 'Strain-dependent cellular immune responses in cattle following Escherichia coli O157:H7 colonization', Infection and Immunity, vol. 82, no. 12, pp. 5117 - 5131. https://doi.org/10.1128/IAI.02462-14

Strain-dependent cellular immune responses in cattle following Escherichia coli O157:H7 colonization. / Corbishley, A; Ahmad, NI; Hughes, K; Hutchings, MR; McAteer, SP; Connelley, TK; Brown, H; Gally, DL; McNeilly, TN.

In: Infection and Immunity, Vol. 82, No. 12, 2014, p. 5117 - 5131.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Strain-dependent cellular immune responses in cattle following Escherichia coli O157:H7 colonization

AU - Corbishley, A

AU - Ahmad, NI

AU - Hughes, K

AU - Hutchings, MR

AU - McAteer, SP

AU - Connelley, TK

AU - Brown, H

AU - Gally, DL

AU - McNeilly, TN

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PY - 2014

Y1 - 2014

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KW - Cattle

KW - Escherichia coli

KW - Immunity

U2 - 10.1128/IAI.02462-14

DO - 10.1128/IAI.02462-14

M3 - Article

VL - 82

SP - 5117

EP - 5131

JO - Infection and Immunity

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SN - 0019-9567

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