Streptococcus caledonicus sp. Nov., isolated from sheep

Geoffrey Foster, Miranda Kirchner, Jakub Muchowski, Nicholas Duggett, Luke Randall, Hazel I. Knight, Adrian M. Whatmore*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Five strains of an unidentified Gram-positive, catalase-negative, chain-forming coccus-shaped organism recovered from sheep in Scotland were characterized using phenotypic and molecular taxonomic methods. Based on morphological and biochemical criteria, the strains were tentatively identified as streptococci but they did not appear to correspond to any recognised species of the genus. Comparative 16S rRNA gene sequencing showed the strains were highly related to each other and confirmed their placement in the genus Streptococcus, with a maximum nucleotide identity of around 97% to extant species. Best matches were with Streptococcus hillyeri followed by Streptococcus porci. Average nucleotide identity and in silico DNA–DNA hybridiza-tion values determined from whole-genome sequence were also consistent with the group representing a novel species. Best matches, again seen to S. hillyeri, followed by S. porci and S. plurextorum, were below accepted cut-off values for species deline-ation. Based on biochemical criteria and molecular genetic evidence, it is proposed that the unknown isolates from sheep be assigned to a new species of the genus Streptococcus as Streptococcus caledonicus sp. nov. The type strain of Streptococcus caledonicus is S784/96/1T=CCUG 73951T=NCTC 14363T.

Original languageEnglish
Article number004081
Pages (from-to)2611-2615
Number of pages5
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume70
Issue number4
Early online date11 Mar 2020
DOIs
Publication statusFirst published - 11 Mar 2020

Keywords

  • Ovine
  • Streptococcus
  • Streptococcus caledonicus
  • Veterinary bacteriology

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    Foster, G., Kirchner, M., Muchowski, J., Duggett, N., Randall, L., Knight, H. I., & Whatmore, A. M. (2020). Streptococcus caledonicus sp. Nov., isolated from sheep. International Journal of Systematic and Evolutionary Microbiology, 70(4), 2611-2615. [004081]. https://doi.org/10.1099/ijsem.0.004081