Strong stability and host specific bacterial community in faeces of ponies

Tina M Blackmore, Alex Dugdale, Caroline McG Argo, Gemma Curtis, Eric Pinloche, Pat A Harris, Hilary J Worgan, Susan E Girdwood, Kirsty Dougal, C Jamie Newbold, Neil R McEwan

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The horse, as a hindgut fermenter, is reliant on its intestinal bacterial population for efficient diet utilisation. However, sudden disturbance of this population can result in severe colic or laminitis, both of which may require euthanasia. This study therefore aimed to determine the temporal stability of the bacterial population of faecal samples from six ponies maintained on a formulated high fibre diet. Bacterial 16S rRNA terminal restriction fragment length polymorphism (TRFLP) analyses of 10 faecal samples collected from 6 ponies at regular intervals over 72 hour trial periods identified a significant pony-specific profile (P<0.001) with strong stability. Within each pony, a significantly different population was found after 11 weeks on the same diet (P<0.001) and with greater intra-individual similarity. Total short chain fatty acid (SCFA) concentration increased in all ponies, but other changes (such as bacterial population diversity measures, individual major SCFA concentration) were significant and dependent on the individual. This study is the first to report the extent of stability of microbes resident in the intestinal tract as represented with such depth and frequency of faecal sampling. In doing so, this provides a baseline from which future trials can be planned and the extent to which results may be interpreted.

Original languageEnglish
Article numbere75079
JournalPLoS ONE
Volume8
Issue number9
DOIs
Publication statusPrint publication - Sep 2013
Externally publishedYes

Fingerprint

Nutrition
bacterial communities
Feces
Volatile Fatty Acids
feces
horses
Population
Fermenters
Diet
short chain fatty acids
Polymorphism
Euthanasia
Colic
Sampling
high fiber diet
laminitis
Fibers
Restriction Fragment Length Polymorphisms
fermenters
colic

Keywords

  • Animals
  • DNA, Bacterial/genetics
  • Fatty Acids, Volatile/metabolism
  • Feces/microbiology
  • Horses/microbiology
  • Intestines/microbiology
  • Metabolomics
  • Microbiota
  • Polymorphism, Restriction Fragment Length
  • Principal Component Analysis
  • RNA, Ribosomal, 16S/genetics
  • Time Factors

Cite this

Blackmore, T. M., Dugdale, A., Argo, C. M., Curtis, G., Pinloche, E., Harris, P. A., ... McEwan, N. R. (2013). Strong stability and host specific bacterial community in faeces of ponies. PLoS ONE, 8(9), [e75079]. https://doi.org/10.1371/journal.pone.0075079
Blackmore, Tina M ; Dugdale, Alex ; Argo, Caroline McG ; Curtis, Gemma ; Pinloche, Eric ; Harris, Pat A ; Worgan, Hilary J ; Girdwood, Susan E ; Dougal, Kirsty ; Newbold, C Jamie ; McEwan, Neil R. / Strong stability and host specific bacterial community in faeces of ponies. In: PLoS ONE. 2013 ; Vol. 8, No. 9.
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Blackmore, TM, Dugdale, A, Argo, CM, Curtis, G, Pinloche, E, Harris, PA, Worgan, HJ, Girdwood, SE, Dougal, K, Newbold, CJ & McEwan, NR 2013, 'Strong stability and host specific bacterial community in faeces of ponies', PLoS ONE, vol. 8, no. 9, e75079. https://doi.org/10.1371/journal.pone.0075079

Strong stability and host specific bacterial community in faeces of ponies. / Blackmore, Tina M; Dugdale, Alex; Argo, Caroline McG; Curtis, Gemma; Pinloche, Eric; Harris, Pat A; Worgan, Hilary J; Girdwood, Susan E; Dougal, Kirsty; Newbold, C Jamie; McEwan, Neil R.

In: PLoS ONE, Vol. 8, No. 9, e75079, 09.2013.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Strong stability and host specific bacterial community in faeces of ponies

AU - Blackmore, Tina M

AU - Dugdale, Alex

AU - Argo, Caroline McG

AU - Curtis, Gemma

AU - Pinloche, Eric

AU - Harris, Pat A

AU - Worgan, Hilary J

AU - Girdwood, Susan E

AU - Dougal, Kirsty

AU - Newbold, C Jamie

AU - McEwan, Neil R

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AB - The horse, as a hindgut fermenter, is reliant on its intestinal bacterial population for efficient diet utilisation. However, sudden disturbance of this population can result in severe colic or laminitis, both of which may require euthanasia. This study therefore aimed to determine the temporal stability of the bacterial population of faecal samples from six ponies maintained on a formulated high fibre diet. Bacterial 16S rRNA terminal restriction fragment length polymorphism (TRFLP) analyses of 10 faecal samples collected from 6 ponies at regular intervals over 72 hour trial periods identified a significant pony-specific profile (P<0.001) with strong stability. Within each pony, a significantly different population was found after 11 weeks on the same diet (P<0.001) and with greater intra-individual similarity. Total short chain fatty acid (SCFA) concentration increased in all ponies, but other changes (such as bacterial population diversity measures, individual major SCFA concentration) were significant and dependent on the individual. This study is the first to report the extent of stability of microbes resident in the intestinal tract as represented with such depth and frequency of faecal sampling. In doing so, this provides a baseline from which future trials can be planned and the extent to which results may be interpreted.

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KW - DNA, Bacterial/genetics

KW - Fatty Acids, Volatile/metabolism

KW - Feces/microbiology

KW - Horses/microbiology

KW - Intestines/microbiology

KW - Metabolomics

KW - Microbiota

KW - Polymorphism, Restriction Fragment Length

KW - Principal Component Analysis

KW - RNA, Ribosomal, 16S/genetics

KW - Time Factors

U2 - 10.1371/journal.pone.0075079

DO - 10.1371/journal.pone.0075079

M3 - Article

C2 - 24040388

VL - 8

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 9

M1 - e75079

ER -

Blackmore TM, Dugdale A, Argo CM, Curtis G, Pinloche E, Harris PA et al. Strong stability and host specific bacterial community in faeces of ponies. PLoS ONE. 2013 Sep;8(9). e75079. https://doi.org/10.1371/journal.pone.0075079