TY - JOUR
T1 - Structural proteins of Helicoverpa armigera densovirus 2 enhance transcription of viral genes through transactivation
AU - Xu, Pengjun
AU - Yuan, He
AU - Yang, Xianming
AU - Graham, Robert I.
AU - Liu, Kaiyu
AU - Wu, Kongming
PY - 2017
Y1 - 2017
N2 - Herein, we report the identification of putative promoters for the non-structural proteins (NS) and capsid structural proteins (VP) of Helicoverpa armigera densovirus (HaDV2) as well as a potential mechanism for how these promoters might be regulated. For the first time, we report that VP is able to transactivate the VP promoter and, to a lesser degree, the NS promoter in densoviruses. In addition to this, another promoter-like sequence designated P2, when co-transfected with the VP gene, enhanced luciferase activity by approximately 35 times compared to a control. This suggests that there are two promoters for VP in HaDV2 and that the VP of parvoviruses might play a more important role in viral transcription than previously appreciated.
AB - Herein, we report the identification of putative promoters for the non-structural proteins (NS) and capsid structural proteins (VP) of Helicoverpa armigera densovirus (HaDV2) as well as a potential mechanism for how these promoters might be regulated. For the first time, we report that VP is able to transactivate the VP promoter and, to a lesser degree, the NS promoter in densoviruses. In addition to this, another promoter-like sequence designated P2, when co-transfected with the VP gene, enhanced luciferase activity by approximately 35 times compared to a control. This suggests that there are two promoters for VP in HaDV2 and that the VP of parvoviruses might play a more important role in viral transcription than previously appreciated.
U2 - 10.1007/s00705-017-3253-0
DO - 10.1007/s00705-017-3253-0
M3 - Report/ Case Report
SN - 0304-8608
VL - 162
SP - 1745
EP - 1750
JO - Archives of Virology
JF - Archives of Virology
ER -