Study of methanogen communities associated with different rumen protozoal populations

Alejandro Belanche, Gabriel de la Fuente, Charles J Newbold

Research output: Contribution to journalArticle

38 Citations (Scopus)
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Abstract

Protozoa-associated methanogens (PAM) are considered one of the most active communities in the rumen methanogenesis. This experiment investigated whether methanogens are sequestrated within rumen protozoa, and structural differences between rumen free-living methanogens and PAM. Rumen protozoa were harvested from totally faunated sheep, and six protozoal fractions (plus free-living microorganisms) were generated by sequential filtration. Holotrich-monofaunated sheep were also used to investigate the holotrich-associated methanogens. Protozoal size determined the number of PAM as big protozoa had 1.7-3.3 times more methanogen DNA than smaller protozoa, but also more endosymbiotic bacteria (2.2- to 3.5-fold times). Thus, similar abundance of methanogens with respect to total bacteria were observed across all protozoal fractions and free-living microorganisms, suggesting that methanogens are not accumulated within rumen protozoa in a greater proportion to that observed in the rumen as a whole. All rumen methanogen communities had similar diversity (22.2 ± 3.4 TRFs). Free-living methanogens composed a conserved community (67% similarity within treatment) in the rumen with similar diversity but different structures than PAM (P < 0.05). On the contrary, PAM constituted a more variable community (48% similarity), which differed between holotrich and total protozoa (P < 0.001). Thus, PAM constitutes a community, which requires further investigation as part of methane mitigation strategies.

Original languageEnglish
Pages (from-to)663-77
Number of pages15
JournalFEMS Microbiology Ecology
Volume90
Issue number3
DOIs
Publication statusPrint publication - Dec 2014
Externally publishedYes

Fingerprint

Rumen
Population
Sheep
Bacteria
Methane
DNA

Bibliographical note

© 2014 The Authors. FEMS Microbiology Ecology published by John Wiley & Sons Ltd on behalf of Federation of European Microbiological Societies.

Keywords

  • Animals
  • Bacteria/metabolism
  • Euryarchaeota/metabolism
  • Methane/metabolism
  • Polymorphism, Restriction Fragment Length
  • Rumen/microbiology
  • Sheep, Domestic/microbiology
  • Symbiosis
  • Trichostomatida/microbiology

Cite this

Belanche, Alejandro ; de la Fuente, Gabriel ; Newbold, Charles J. / Study of methanogen communities associated with different rumen protozoal populations. In: FEMS Microbiology Ecology. 2014 ; Vol. 90, No. 3. pp. 663-77.
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abstract = "Protozoa-associated methanogens (PAM) are considered one of the most active communities in the rumen methanogenesis. This experiment investigated whether methanogens are sequestrated within rumen protozoa, and structural differences between rumen free-living methanogens and PAM. Rumen protozoa were harvested from totally faunated sheep, and six protozoal fractions (plus free-living microorganisms) were generated by sequential filtration. Holotrich-monofaunated sheep were also used to investigate the holotrich-associated methanogens. Protozoal size determined the number of PAM as big protozoa had 1.7-3.3 times more methanogen DNA than smaller protozoa, but also more endosymbiotic bacteria (2.2- to 3.5-fold times). Thus, similar abundance of methanogens with respect to total bacteria were observed across all protozoal fractions and free-living microorganisms, suggesting that methanogens are not accumulated within rumen protozoa in a greater proportion to that observed in the rumen as a whole. All rumen methanogen communities had similar diversity (22.2 ± 3.4 TRFs). Free-living methanogens composed a conserved community (67{\%} similarity within treatment) in the rumen with similar diversity but different structures than PAM (P < 0.05). On the contrary, PAM constituted a more variable community (48{\%} similarity), which differed between holotrich and total protozoa (P < 0.001). Thus, PAM constitutes a community, which requires further investigation as part of methane mitigation strategies.",
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Study of methanogen communities associated with different rumen protozoal populations. / Belanche, Alejandro; de la Fuente, Gabriel; Newbold, Charles J.

In: FEMS Microbiology Ecology, Vol. 90, No. 3, 12.2014, p. 663-77.

Research output: Contribution to journalArticle

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T1 - Study of methanogen communities associated with different rumen protozoal populations

AU - Belanche, Alejandro

AU - de la Fuente, Gabriel

AU - Newbold, Charles J

N1 - © 2014 The Authors. FEMS Microbiology Ecology published by John Wiley & Sons Ltd on behalf of Federation of European Microbiological Societies.

PY - 2014/12

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KW - Euryarchaeota/metabolism

KW - Methane/metabolism

KW - Polymorphism, Restriction Fragment Length

KW - Rumen/microbiology

KW - Sheep, Domestic/microbiology

KW - Symbiosis

KW - Trichostomatida/microbiology

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