Sustainable Nanoparticles from Stephania glabra and Analysis of Their Anticancer Potential on 2D and 3D Models of Prostate Cancer

Prachi Vaid; Adesh K. Saini; Raju Kumar Gupta; Eshu Singhal Sinha; Deepak Sharma; Walaa F. Alsanie; Vijay Kumar Thakur; Reena V. Saini

doi:10.1007/s12010-023-04700-w

Sustainable Nanoparticles from Stephania glabra and Analysis of Their Anticancer Potential on 2D and 3D Models of Prostate Cancer

Prachi Vaid, Adesh K. Saini, Raju Kumar Gupta, Eshu Singhal Sinha, Deepak Sharma, Walaa F. Alsanie, Vijay Kumar Thakur, Reena V. Saini^*

^*Corresponding author for this work

Biorefining And Advanced Materials Research Centre

Research output: Contribution to journal › Article › peer-review

Abstract

In pursuit of a novel effective treatment for prostate cancer, methanolic extract of Stephania glabra tubers (Sg-ME) was utilized to fabricate silver (Sg-AgNP), copper oxide (Sg-CuONP), and silver-copper bimetallic nanoparticles (Sg-BNP). The characterization of the nanoparticles confirmed spherical shape with average diameters of 30.72, 32.19, and 25.59 nm of Sg-AgNP, Sg-CuONP, and Sg-BNP, respectively. Interestingly, these nanoparticles exhibited significant cytotoxicity toward the prostate cancer (PC3) cell line while being non-toxic toward normal cells. The nanoparticles were capable of inducing apoptosis in PC3 cells by enhancing reactive oxygen species (ROS) generation and mitochondrial depolarization. Furthermore, the shrinkage of 3D prostate tumor spheroids was observed after 4 days of treatment with these green nanoparticles. The 3D model system was less susceptible to nanoparticles as compared to the 2D model system. Sg-BNP showed the highest anticancer potential on 2D and 3D prostate cancer models.

Original language	English
Journal	Applied Biochemistry and Biotechnology
Early online date	8 Sept 2023
DOIs	https://doi.org/10.1007/s12010-023-04700-w
Publication status	First published - 8 Sept 2023

Bibliographical note

Publisher Copyright:
© 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

3D tumor model
Apoptosis
Bimetallic nanoparticles
Copper oxide nanoparticles
Prostate cancer
Silver nanoparticles
Stephania glabra

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s12010-023-04700-w

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title = "Sustainable Nanoparticles from Stephania glabra and Analysis of Their Anticancer Potential on 2D and 3D Models of Prostate Cancer",

abstract = "In pursuit of a novel effective treatment for prostate cancer, methanolic extract of Stephania glabra tubers (Sg-ME) was utilized to fabricate silver (Sg-AgNP), copper oxide (Sg-CuONP), and silver-copper bimetallic nanoparticles (Sg-BNP). The characterization of the nanoparticles confirmed spherical shape with average diameters of 30.72, 32.19, and 25.59 nm of Sg-AgNP, Sg-CuONP, and Sg-BNP, respectively. Interestingly, these nanoparticles exhibited significant cytotoxicity toward the prostate cancer (PC3) cell line while being non-toxic toward normal cells. The nanoparticles were capable of inducing apoptosis in PC3 cells by enhancing reactive oxygen species (ROS) generation and mitochondrial depolarization. Furthermore, the shrinkage of 3D prostate tumor spheroids was observed after 4 days of treatment with these green nanoparticles. The 3D model system was less susceptible to nanoparticles as compared to the 2D model system. Sg-BNP showed the highest anticancer potential on 2D and 3D prostate cancer models.",

keywords = "3D tumor model, Apoptosis, Bimetallic nanoparticles, Copper oxide nanoparticles, Prostate cancer, Silver nanoparticles, Stephania glabra",

author = "Prachi Vaid and Saini, {Adesh K.} and Gupta, {Raju Kumar} and Sinha, {Eshu Singhal} and Deepak Sharma and Alsanie, {Walaa F.} and Thakur, {Vijay Kumar} and Saini, {Reena V.}",

note = " Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.",

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doi = "10.1007/s12010-023-04700-w",

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AU - Saini, Adesh K.

AU - Gupta, Raju Kumar

AU - Sinha, Eshu Singhal

AU - Sharma, Deepak

AU - Alsanie, Walaa F.

AU - Thakur, Vijay Kumar

AU - Saini, Reena V.

PY - 2023/9/8

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AB - In pursuit of a novel effective treatment for prostate cancer, methanolic extract of Stephania glabra tubers (Sg-ME) was utilized to fabricate silver (Sg-AgNP), copper oxide (Sg-CuONP), and silver-copper bimetallic nanoparticles (Sg-BNP). The characterization of the nanoparticles confirmed spherical shape with average diameters of 30.72, 32.19, and 25.59 nm of Sg-AgNP, Sg-CuONP, and Sg-BNP, respectively. Interestingly, these nanoparticles exhibited significant cytotoxicity toward the prostate cancer (PC3) cell line while being non-toxic toward normal cells. The nanoparticles were capable of inducing apoptosis in PC3 cells by enhancing reactive oxygen species (ROS) generation and mitochondrial depolarization. Furthermore, the shrinkage of 3D prostate tumor spheroids was observed after 4 days of treatment with these green nanoparticles. The 3D model system was less susceptible to nanoparticles as compared to the 2D model system. Sg-BNP showed the highest anticancer potential on 2D and 3D prostate cancer models.

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Sustainable Nanoparticles from Stephania glabra and Analysis of Their Anticancer Potential on 2D and 3D Models of Prostate Cancer

Abstract

Bibliographical note

Keywords

UN SDGs

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