SWEET11b transports both sugar and cytokinin in developing barley grains

Volodymyr Radchuk, Zeinu M Belew, Andre Gündel, Simon Mayer, Alexander Hilo, Goetz Hensel, Rajiv Sharma, Kerstin Neumann, Stefan Ortleb, Steffen Wagner, Aleksandra Muszynska, Christoph Crocoll, Deyang Xu, Iris Hoffie, Jochen Kumlehn, Joerg Fuchs, Fritz F Peleke, Jedrzej J Szymanski, Hardy Rolletschek, Hussam H Nour-EldinLjudmilla Borisjuk

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)
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Abstract

Even though Sugars Will Eventually be Exported Transporters (SWEETs) have been found in every sequenced plant genome, a comprehensive understanding of their functionality is lacking. In this study, we focused on the SWEET family of barley (Hordeum vulgare). A radiotracer assay revealed that expressing HvSWEET11b in African clawed frog (Xenopus laevis) oocytes facilitated the bidirectional transfer of not only just sucrose and glucose, but also cytokinin. Barley plants harboring a loss-of-function mutation of HvSWEET11b could not set viable grains, while the distribution of sucrose and cytokinin was altered in developing grains of plants in which the gene was knocked down. Sucrose allocation within transgenic grains was disrupted, which is consistent with the changes to the cytokinin gradient across grains, as visualized by magnetic resonance imaging and Fourier transform infrared spectroscopy microimaging. Decreasing HvSWEET11b expression in developing grains reduced overall grain size, sink strength, the number of endopolyploid endosperm cells, and the contents of starch and protein. The control exerted by HvSWEET11b over sugars and cytokinins likely predetermines their synergy, resulting in adjustments to the grain's biochemistry and transcriptome.

Original languageEnglish
Pages (from-to)2186-2207
Number of pages22
JournalPlant Cell
Volume35
Issue number6
Early online date1 Mar 2023
DOIs
Publication statusPrint publication - Jun 2023

Bibliographical note

© The Author(s) 2023. Published by Oxford University Press on behalf of American Society of Plant Biologists.

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