Temporal and spatial variation in Anaplasma phagocytophilum infection in Swedish moose (Alces alces)

J Malmsten, D Gavier Widen, G Rydevik, L Yon, MR Hutchings, CG Thulin, L Soderquist, A Aspan, S Stuen, AM Dalin

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Abstract

The occurrence of Anaplasma phagocytophilum was investigated in spleen and serum samples from Swedish moose (Alces alces) in southern Sweden (island and mainland). Samples were analysed for presence of A. phagocytophilum DNA by real-time PCR (n=263), and for Anaplasma antibodies with ELISA serology (n=234). All serum samples had antibodies against A. phagocytophilum. The mean DNA-based prevalence was 26·3%, and significant (P<0·01) temporal, and spatial variation was found. Island moose had significantly (P<0·001) higher prevalence of A. phagocytophilum DNA than moose from the mainland areas. Two samples were sequenced to determine genetic variation in the 16S rRNA and groESL genes. Genetic sequence similarity with the human granulocytic anaplasmosis agent, equine granulocytic ehrlichiosis agent, and different wildlife-associated A. phagocytophilum variants were observed in the 16S rRNA and groESL genes. Our study shows that moose are exposed to A. phagocytophilum in Sweden, and represent a potential wildlife reservoir of the pathogen.
Original languageEnglish
Pages (from-to)1205 - 1213
Number of pages9
JournalEpidemiology and Infection
Volume142
Issue number6
DOIs
Publication statusFirst published - 2014

Keywords

  • Ehrlichia
  • Epidemiology
  • HGA
  • Moose
  • PCR
  • Serology
  • Tick-borne fever

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    Malmsten, J., Gavier Widen, D., Rydevik, G., Yon, L., Hutchings, MR., Thulin, CG., Soderquist, L., Aspan, A., Stuen, S., & Dalin, AM. (2014). Temporal and spatial variation in Anaplasma phagocytophilum infection in Swedish moose (Alces alces). Epidemiology and Infection, 142(6), 1205 - 1213. https://doi.org/10.1017/S0950268813002094