The construction of a substitution library of recombinant backcross lines in Brassica oleracea for the precision mapping of quantitative trait loci

L. D. Ramsay, D. E. Jennings, M. J. Kearsey, D. F. Marshall, E. J. R. Bohuon, A. E. Arthur, D. J. Lydiate

Research output: Contribution to journalArticlepeer-review

68 Citations (Scopus)

Abstract

The currently available methods for locating quantitative trait loci (QTLs) and measuring their effects in segregating populations lack precision unless individual QTLs have very high heritabilities. The use of recombinant backcross lines containing short regions of donor chromosome introgressed into a constant recipient background permits QTLs to be located with greater precision. The present paper describes the use of molecular markers to introgress defined short regions of chromosome from a donor doubled haploid calabrese line of Brassica oleracea (var. italica) into a recipient short generation variety (Brassica oleracea var. alboglabra). We demonstrate that in just two or three generations of backcrossing, combined with selection for mapped molecular markers, the generation of a library of recombinant backcross lines is feasible. The possible use and refinement of these lines are discussed. Key words : backcrossing, Brassica oleracea, introgression, molecular markers, near-isogenic lines, QTL mapping...
Original languageEnglish
Pages (from-to)558-567
Number of pages10
JournalGenome
Volume39
Issue number3
DOIs
Publication statusPrint publication - Jun 1996
Externally publishedYes

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