The determination of amino-acids colorimetrically by the ninhydrin reaction

A. M. Smith*, A. H. Agiza

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)


The coloured compound produced in the reaction between amino-acids and ninhydrin can be used as a quantitative measure of the amino-acid decomposed. The depth of colour is not the same for all the common amino-acids, but the results are reproducible to within 3 per cent. and the curve is linear for all the acids examined in the range 5 to 25 μg of amino nitrogen. Hence the reaction provides a convenient method of determining the sub-micro quantities of amino-acid obtained from a drop of a protein hydrolysate fractionated by two-dimensional chromatography on paper.

Original languageEnglish
Pages (from-to)623-627
Number of pages5
JournalThe Analyst
Issue number908
Publication statusPrint publication - 1 Dec 1951


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