Abstract
The coloured compound produced in the reaction between amino-acids and ninhydrin can be used as a quantitative measure of the amino-acid decomposed. The depth of colour is not the same for all the common amino-acids, but the results are reproducible to within 3 per cent. and the curve is linear for all the acids examined in the range 5 to 25 μg of amino nitrogen. Hence the reaction provides a convenient method of determining the sub-micro quantities of amino-acid obtained from a drop of a protein hydrolysate fractionated by two-dimensional chromatography on paper.
| Original language | English |
|---|---|
| Pages (from-to) | 623-627 |
| Number of pages | 5 |
| Journal | The Analyst |
| Volume | 76 |
| Issue number | 908 |
| DOIs | |
| Publication status | Print publication - 1 Dec 1951 |