The effect of incremental levels of dietary nitrate on methane emissions in Holstein steers and performance in Nelore bulls

J R Newbold, S M van Zijderveld, R B A Hulshof, W B Fokkink, R A Leng, P Terencio, W J Powers, P S J van Adrichem, N D Paton, H B Perdok

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Two experiments were conducted to study effects of dietary nitrate on enteric methane production, blood methemoglobin concentration, and growth rate in cattle. In Exp. 1, 36 Holstein steers (288 ± 25 kg BW) were fed increasing levels of dietary nitrate (6 levels; 0 to 3.0% of feed DM) in corn silage-based total mixed rations. Nitrate was introduced gradually in a 25-d adaptation period before methane production was determined in environmentally controlled rooms. In the rooms, feed intake was restricted and similar among all treatments. Methane production (g/d) decreased linearly as dietary nitrate concentration increased (P < 0.01). The apparent efficiency (measured methane reduction divided by potential methane reduction) with which enteric methane was mitigated was 49%. Blood methemoglobin levels increased with increasing nitrate dose. In Exp. 2, 300 Nelore bulls (392 ± 28 kg) were fed increasing levels of nitrate (6 levels; 0 to 2.4% of feed DM) in high-concentrate total mixed rations offered ad libitum. Feed intake decreased linearly with increasing level of dietary nitrate (P < 0.01). However, ADG was not affected by nitrate dose (P = 0.54), resulting in a linear improvement in G:F (P = 0.03) as dietary nitrate level increased. Carcass dressing percentage showed a quadratic response to incremental dietary nitrate, reaching the highest value at 0.96% of NO3/kg DM (P = 0.04).

Original languageEnglish
Pages (from-to)5032-40
Number of pages9
JournalJournal of Animal Science
Volume92
Issue number11
DOIs
Publication statusPrint publication - Nov 2014
Externally publishedYes

Fingerprint

Nellore
Methane
Nitrates
methane
bulls
Holstein
nitrates
methane production
Methemoglobin
total mixed rations
feed intake
Silage
dressing percentage
blood
Bandages
dosage
corn silage
Zea mays
concentrates
corn

Keywords

  • Animal Nutritional Physiological Phenomena
  • Animals
  • Cattle/growth & development
  • Diet/veterinary
  • Male
  • Methane/metabolism
  • Nitrates/administration & dosage
  • Silage/analysis

Cite this

Newbold, J. R., van Zijderveld, S. M., Hulshof, R. B. A., Fokkink, W. B., Leng, R. A., Terencio, P., ... Perdok, H. B. (2014). The effect of incremental levels of dietary nitrate on methane emissions in Holstein steers and performance in Nelore bulls. Journal of Animal Science, 92(11), 5032-40. https://doi.org/10.2527/jas.2014-7677
Newbold, J R ; van Zijderveld, S M ; Hulshof, R B A ; Fokkink, W B ; Leng, R A ; Terencio, P ; Powers, W J ; van Adrichem, P S J ; Paton, N D ; Perdok, H B. / The effect of incremental levels of dietary nitrate on methane emissions in Holstein steers and performance in Nelore bulls. In: Journal of Animal Science. 2014 ; Vol. 92, No. 11. pp. 5032-40.
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abstract = "Two experiments were conducted to study effects of dietary nitrate on enteric methane production, blood methemoglobin concentration, and growth rate in cattle. In Exp. 1, 36 Holstein steers (288 ± 25 kg BW) were fed increasing levels of dietary nitrate (6 levels; 0 to 3.0{\%} of feed DM) in corn silage-based total mixed rations. Nitrate was introduced gradually in a 25-d adaptation period before methane production was determined in environmentally controlled rooms. In the rooms, feed intake was restricted and similar among all treatments. Methane production (g/d) decreased linearly as dietary nitrate concentration increased (P < 0.01). The apparent efficiency (measured methane reduction divided by potential methane reduction) with which enteric methane was mitigated was 49{\%}. Blood methemoglobin levels increased with increasing nitrate dose. In Exp. 2, 300 Nelore bulls (392 ± 28 kg) were fed increasing levels of nitrate (6 levels; 0 to 2.4{\%} of feed DM) in high-concentrate total mixed rations offered ad libitum. Feed intake decreased linearly with increasing level of dietary nitrate (P < 0.01). However, ADG was not affected by nitrate dose (P = 0.54), resulting in a linear improvement in G:F (P = 0.03) as dietary nitrate level increased. Carcass dressing percentage showed a quadratic response to incremental dietary nitrate, reaching the highest value at 0.96{\%} of NO3/kg DM (P = 0.04).",
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year = "2014",
month = "11",
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Newbold, JR, van Zijderveld, SM, Hulshof, RBA, Fokkink, WB, Leng, RA, Terencio, P, Powers, WJ, van Adrichem, PSJ, Paton, ND & Perdok, HB 2014, 'The effect of incremental levels of dietary nitrate on methane emissions in Holstein steers and performance in Nelore bulls', Journal of Animal Science, vol. 92, no. 11, pp. 5032-40. https://doi.org/10.2527/jas.2014-7677

The effect of incremental levels of dietary nitrate on methane emissions in Holstein steers and performance in Nelore bulls. / Newbold, J R; van Zijderveld, S M; Hulshof, R B A; Fokkink, W B; Leng, R A; Terencio, P; Powers, W J; van Adrichem, P S J; Paton, N D; Perdok, H B.

In: Journal of Animal Science, Vol. 92, No. 11, 11.2014, p. 5032-40.

Research output: Contribution to journalArticle

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T1 - The effect of incremental levels of dietary nitrate on methane emissions in Holstein steers and performance in Nelore bulls

AU - Newbold, J R

AU - van Zijderveld, S M

AU - Hulshof, R B A

AU - Fokkink, W B

AU - Leng, R A

AU - Terencio, P

AU - Powers, W J

AU - van Adrichem, P S J

AU - Paton, N D

AU - Perdok, H B

PY - 2014/11

Y1 - 2014/11

N2 - Two experiments were conducted to study effects of dietary nitrate on enteric methane production, blood methemoglobin concentration, and growth rate in cattle. In Exp. 1, 36 Holstein steers (288 ± 25 kg BW) were fed increasing levels of dietary nitrate (6 levels; 0 to 3.0% of feed DM) in corn silage-based total mixed rations. Nitrate was introduced gradually in a 25-d adaptation period before methane production was determined in environmentally controlled rooms. In the rooms, feed intake was restricted and similar among all treatments. Methane production (g/d) decreased linearly as dietary nitrate concentration increased (P < 0.01). The apparent efficiency (measured methane reduction divided by potential methane reduction) with which enteric methane was mitigated was 49%. Blood methemoglobin levels increased with increasing nitrate dose. In Exp. 2, 300 Nelore bulls (392 ± 28 kg) were fed increasing levels of nitrate (6 levels; 0 to 2.4% of feed DM) in high-concentrate total mixed rations offered ad libitum. Feed intake decreased linearly with increasing level of dietary nitrate (P < 0.01). However, ADG was not affected by nitrate dose (P = 0.54), resulting in a linear improvement in G:F (P = 0.03) as dietary nitrate level increased. Carcass dressing percentage showed a quadratic response to incremental dietary nitrate, reaching the highest value at 0.96% of NO3/kg DM (P = 0.04).

AB - Two experiments were conducted to study effects of dietary nitrate on enteric methane production, blood methemoglobin concentration, and growth rate in cattle. In Exp. 1, 36 Holstein steers (288 ± 25 kg BW) were fed increasing levels of dietary nitrate (6 levels; 0 to 3.0% of feed DM) in corn silage-based total mixed rations. Nitrate was introduced gradually in a 25-d adaptation period before methane production was determined in environmentally controlled rooms. In the rooms, feed intake was restricted and similar among all treatments. Methane production (g/d) decreased linearly as dietary nitrate concentration increased (P < 0.01). The apparent efficiency (measured methane reduction divided by potential methane reduction) with which enteric methane was mitigated was 49%. Blood methemoglobin levels increased with increasing nitrate dose. In Exp. 2, 300 Nelore bulls (392 ± 28 kg) were fed increasing levels of nitrate (6 levels; 0 to 2.4% of feed DM) in high-concentrate total mixed rations offered ad libitum. Feed intake decreased linearly with increasing level of dietary nitrate (P < 0.01). However, ADG was not affected by nitrate dose (P = 0.54), resulting in a linear improvement in G:F (P = 0.03) as dietary nitrate level increased. Carcass dressing percentage showed a quadratic response to incremental dietary nitrate, reaching the highest value at 0.96% of NO3/kg DM (P = 0.04).

KW - Animal Nutritional Physiological Phenomena

KW - Animals

KW - Cattle/growth & development

KW - Diet/veterinary

KW - Male

KW - Methane/metabolism

KW - Nitrates/administration & dosage

KW - Silage/analysis

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DO - 10.2527/jas.2014-7677

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JF - Journal of Animal Science

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