The effect of LPS and ketoprofen on social behaviour and brain physiology in group housed pigs

Christina Veit, Andrew Janczak, Birgit Ranheim, Anna Valros, DA Sandercock, Janicke Nordgreen*

*Corresponding author for this work

Research output: Contribution to conferenceAbstract

Abstract

Poor health is a risk factor for tail biting but the mechanism behind this link is still unclear. An injection with lipopolysaccharide (LPS) can be used to model aspects of sickness. Recent studies showed more tail- and ear- directed behaviour in LPS injected pigs compared to saline injected pigs. The aim of this study was to evaluate social behaviour and stress physiology of pigs after LPS injection [1.2 µg/kg], and to test the effect of a nonsteroidal anti-inflammatory drug (NSAID) on the effects of LPS. The experiment was approved by the national animal research authority (FOTS id 15232). Fifty-two female pigs (11-12 weeks) were randomly allocated to four treatments all comprising two injections: Saline-Saline (SS), Saline-LPS (SL), Ketoprofen-Saline (KS), Ketoprofen-LPS (KL). The first substance was administered intramuscularly (i.m.). The latter was administered intravenously (i.v.) on average 60 ± 14 min afterwards in an ear vein during fixation with a mouth snare. Pigs were marked on the back for individual identification and video recorded. Activity was scan sampled every 5 min for 6 h after the last i.v. injection in the pen. Social behaviour was observed continuously in 10 x 15 min intervals between 8 am and 5 pm at baseline and one and two days after i.v. injection with focus on ear and tail exploration as well as manipulation of other body parts. Data were analysed by a mixed model to account for repeated measures and baseline behaviour. Saliva was sampled at baseline and at 4, 24, 48, 72 h after the i.v. injection and analysed for cortisol. Salivary cortisol was significantly higher in SL pigs (1067.6 ± 77.8 pg/ml) compared to KL pigs (641.4 ± 77.8 pg/ml; student’s t-test: p < 0.001), SS pigs (418.0 ± 84.6 pg/ml; p < 0.001) and KS pigs (258.0 ± 77.8 pg/ml; p < 0.001) at 4 h post injection (F(treatment*time point)12,182.8 = 4.65; p < 0.001). SL pigs showed more sternal recumbancy (296 counts) and were less active (52) than SS pigs (lying inactive sternal: 149 | active: 186, student’s t-test for both models respectively: p < 0.001), KL pigs (174 | 217, p < 0.001), and KS pigs (151 | 215, p < 0.001) during 6 h post injection (lying inactive sternal F(treatment)3,48 = 9.26; p < 0.001; active F(treatment)3,48 = 14.66; p < 0.001). Treatment effects on social behaviour were not consistent. SL pigs performed longer ear exploration (sum per day: 1702.0 s) than SS pigs (465.9 s) two days after i.v. injection, (student’s t-test: p = 0.02; F(treatment*day)6,88 = 1.49, p = 0.19). They did not differ at baseline. The duration of tail exploration (F(treatment*day)6, 87.58 = 0.23, p = 0.97) and manipulation of other body parts (F(treatment*day)6, 88 = 0.36, p = 0.90) did not differ between treatments. LPS activated the HPA-axis (measured 4 h after injection) and elicited so-called sickness behaviour within 6 h after injection as indicated by lower activity in LPS injected pigs.
Original languageEnglish
Number of pages1
Publication statusAccepted/In press - 15 Apr 2019
Event53rd Congress of the International Society for Applied Ethology (ISAE) - Bergen, Norway
Duration: 5 Aug 20199 Aug 2019
http://www.isae2019.com/

Conference

Conference53rd Congress of the International Society for Applied Ethology (ISAE)
CountryNorway
CityBergen
Period5/08/199/08/19
Internet address

Fingerprint

ketoprofen
social behavior
lipopolysaccharides
physiology
brain
swine
injection
ears
tail
cortisol
animal research
nonsteroidal anti-inflammatory agents
back (body region)

Keywords

  • Tail biting
  • Risk factors
  • Pig production
  • Poor health
  • Lipopolysaccharide
  • Inflammation
  • NSAID
  • Social Behaviour
  • Salivary cortisol
  • HPA-axis
  • Tail lesions
  • Pig welfare
  • Pain
  • Brain
  • Neuroinflammation

Cite this

Veit, C., Janczak, A., Ranheim, B., Valros, A., Sandercock, DA., & Nordgreen, J. (Accepted/In press). The effect of LPS and ketoprofen on social behaviour and brain physiology in group housed pigs. Abstract from 53rd Congress of the International Society for Applied Ethology (ISAE), Bergen, Norway.
Veit, Christina ; Janczak, Andrew ; Ranheim, Birgit ; Valros, Anna ; Sandercock, DA ; Nordgreen, Janicke. / The effect of LPS and ketoprofen on social behaviour and brain physiology in group housed pigs. Abstract from 53rd Congress of the International Society for Applied Ethology (ISAE), Bergen, Norway.1 p.
@conference{f31f2105331a42be8b04e74d198fe5a7,
title = "The effect of LPS and ketoprofen on social behaviour and brain physiology in group housed pigs",
abstract = "Poor health is a risk factor for tail biting but the mechanism behind this link is still unclear. An injection with lipopolysaccharide (LPS) can be used to model aspects of sickness. Recent studies showed more tail- and ear- directed behaviour in LPS injected pigs compared to saline injected pigs. The aim of this study was to evaluate social behaviour and stress physiology of pigs after LPS injection [1.2 µg/kg], and to test the effect of a nonsteroidal anti-inflammatory drug (NSAID) on the effects of LPS. The experiment was approved by the national animal research authority (FOTS id 15232). Fifty-two female pigs (11-12 weeks) were randomly allocated to four treatments all comprising two injections: Saline-Saline (SS), Saline-LPS (SL), Ketoprofen-Saline (KS), Ketoprofen-LPS (KL). The first substance was administered intramuscularly (i.m.). The latter was administered intravenously (i.v.) on average 60 ± 14 min afterwards in an ear vein during fixation with a mouth snare. Pigs were marked on the back for individual identification and video recorded. Activity was scan sampled every 5 min for 6 h after the last i.v. injection in the pen. Social behaviour was observed continuously in 10 x 15 min intervals between 8 am and 5 pm at baseline and one and two days after i.v. injection with focus on ear and tail exploration as well as manipulation of other body parts. Data were analysed by a mixed model to account for repeated measures and baseline behaviour. Saliva was sampled at baseline and at 4, 24, 48, 72 h after the i.v. injection and analysed for cortisol. Salivary cortisol was significantly higher in SL pigs (1067.6 ± 77.8 pg/ml) compared to KL pigs (641.4 ± 77.8 pg/ml; student’s t-test: p < 0.001), SS pigs (418.0 ± 84.6 pg/ml; p < 0.001) and KS pigs (258.0 ± 77.8 pg/ml; p < 0.001) at 4 h post injection (F(treatment*time point)12,182.8 = 4.65; p < 0.001). SL pigs showed more sternal recumbancy (296 counts) and were less active (52) than SS pigs (lying inactive sternal: 149 | active: 186, student’s t-test for both models respectively: p < 0.001), KL pigs (174 | 217, p < 0.001), and KS pigs (151 | 215, p < 0.001) during 6 h post injection (lying inactive sternal F(treatment)3,48 = 9.26; p < 0.001; active F(treatment)3,48 = 14.66; p < 0.001). Treatment effects on social behaviour were not consistent. SL pigs performed longer ear exploration (sum per day: 1702.0 s) than SS pigs (465.9 s) two days after i.v. injection, (student’s t-test: p = 0.02; F(treatment*day)6,88 = 1.49, p = 0.19). They did not differ at baseline. The duration of tail exploration (F(treatment*day)6, 87.58 = 0.23, p = 0.97) and manipulation of other body parts (F(treatment*day)6, 88 = 0.36, p = 0.90) did not differ between treatments. LPS activated the HPA-axis (measured 4 h after injection) and elicited so-called sickness behaviour within 6 h after injection as indicated by lower activity in LPS injected pigs.",
keywords = "Tail biting, Risk factors, Pig production, Poor health, Lipopolysaccharide, Inflammation, NSAID, Social Behaviour, Salivary cortisol, HPA-axis, Tail lesions, Pig welfare, Pain, Brain, Neuroinflammation",
author = "Christina Veit and Andrew Janczak and Birgit Ranheim and Anna Valros and DA Sandercock and Janicke Nordgreen",
year = "2019",
month = "4",
day = "15",
language = "English",
note = "53rd Congress of the International Society for Applied Ethology (ISAE) ; Conference date: 05-08-2019 Through 09-08-2019",
url = "http://www.isae2019.com/",

}

Veit, C, Janczak, A, Ranheim, B, Valros, A, Sandercock, DA & Nordgreen, J 2019, 'The effect of LPS and ketoprofen on social behaviour and brain physiology in group housed pigs', 53rd Congress of the International Society for Applied Ethology (ISAE), Bergen, Norway, 5/08/19 - 9/08/19.

The effect of LPS and ketoprofen on social behaviour and brain physiology in group housed pigs. / Veit, Christina; Janczak, Andrew; Ranheim, Birgit; Valros, Anna; Sandercock, DA; Nordgreen, Janicke.

2019. Abstract from 53rd Congress of the International Society for Applied Ethology (ISAE), Bergen, Norway.

Research output: Contribution to conferenceAbstract

TY - CONF

T1 - The effect of LPS and ketoprofen on social behaviour and brain physiology in group housed pigs

AU - Veit, Christina

AU - Janczak, Andrew

AU - Ranheim, Birgit

AU - Valros, Anna

AU - Sandercock, DA

AU - Nordgreen, Janicke

PY - 2019/4/15

Y1 - 2019/4/15

N2 - Poor health is a risk factor for tail biting but the mechanism behind this link is still unclear. An injection with lipopolysaccharide (LPS) can be used to model aspects of sickness. Recent studies showed more tail- and ear- directed behaviour in LPS injected pigs compared to saline injected pigs. The aim of this study was to evaluate social behaviour and stress physiology of pigs after LPS injection [1.2 µg/kg], and to test the effect of a nonsteroidal anti-inflammatory drug (NSAID) on the effects of LPS. The experiment was approved by the national animal research authority (FOTS id 15232). Fifty-two female pigs (11-12 weeks) were randomly allocated to four treatments all comprising two injections: Saline-Saline (SS), Saline-LPS (SL), Ketoprofen-Saline (KS), Ketoprofen-LPS (KL). The first substance was administered intramuscularly (i.m.). The latter was administered intravenously (i.v.) on average 60 ± 14 min afterwards in an ear vein during fixation with a mouth snare. Pigs were marked on the back for individual identification and video recorded. Activity was scan sampled every 5 min for 6 h after the last i.v. injection in the pen. Social behaviour was observed continuously in 10 x 15 min intervals between 8 am and 5 pm at baseline and one and two days after i.v. injection with focus on ear and tail exploration as well as manipulation of other body parts. Data were analysed by a mixed model to account for repeated measures and baseline behaviour. Saliva was sampled at baseline and at 4, 24, 48, 72 h after the i.v. injection and analysed for cortisol. Salivary cortisol was significantly higher in SL pigs (1067.6 ± 77.8 pg/ml) compared to KL pigs (641.4 ± 77.8 pg/ml; student’s t-test: p < 0.001), SS pigs (418.0 ± 84.6 pg/ml; p < 0.001) and KS pigs (258.0 ± 77.8 pg/ml; p < 0.001) at 4 h post injection (F(treatment*time point)12,182.8 = 4.65; p < 0.001). SL pigs showed more sternal recumbancy (296 counts) and were less active (52) than SS pigs (lying inactive sternal: 149 | active: 186, student’s t-test for both models respectively: p < 0.001), KL pigs (174 | 217, p < 0.001), and KS pigs (151 | 215, p < 0.001) during 6 h post injection (lying inactive sternal F(treatment)3,48 = 9.26; p < 0.001; active F(treatment)3,48 = 14.66; p < 0.001). Treatment effects on social behaviour were not consistent. SL pigs performed longer ear exploration (sum per day: 1702.0 s) than SS pigs (465.9 s) two days after i.v. injection, (student’s t-test: p = 0.02; F(treatment*day)6,88 = 1.49, p = 0.19). They did not differ at baseline. The duration of tail exploration (F(treatment*day)6, 87.58 = 0.23, p = 0.97) and manipulation of other body parts (F(treatment*day)6, 88 = 0.36, p = 0.90) did not differ between treatments. LPS activated the HPA-axis (measured 4 h after injection) and elicited so-called sickness behaviour within 6 h after injection as indicated by lower activity in LPS injected pigs.

AB - Poor health is a risk factor for tail biting but the mechanism behind this link is still unclear. An injection with lipopolysaccharide (LPS) can be used to model aspects of sickness. Recent studies showed more tail- and ear- directed behaviour in LPS injected pigs compared to saline injected pigs. The aim of this study was to evaluate social behaviour and stress physiology of pigs after LPS injection [1.2 µg/kg], and to test the effect of a nonsteroidal anti-inflammatory drug (NSAID) on the effects of LPS. The experiment was approved by the national animal research authority (FOTS id 15232). Fifty-two female pigs (11-12 weeks) were randomly allocated to four treatments all comprising two injections: Saline-Saline (SS), Saline-LPS (SL), Ketoprofen-Saline (KS), Ketoprofen-LPS (KL). The first substance was administered intramuscularly (i.m.). The latter was administered intravenously (i.v.) on average 60 ± 14 min afterwards in an ear vein during fixation with a mouth snare. Pigs were marked on the back for individual identification and video recorded. Activity was scan sampled every 5 min for 6 h after the last i.v. injection in the pen. Social behaviour was observed continuously in 10 x 15 min intervals between 8 am and 5 pm at baseline and one and two days after i.v. injection with focus on ear and tail exploration as well as manipulation of other body parts. Data were analysed by a mixed model to account for repeated measures and baseline behaviour. Saliva was sampled at baseline and at 4, 24, 48, 72 h after the i.v. injection and analysed for cortisol. Salivary cortisol was significantly higher in SL pigs (1067.6 ± 77.8 pg/ml) compared to KL pigs (641.4 ± 77.8 pg/ml; student’s t-test: p < 0.001), SS pigs (418.0 ± 84.6 pg/ml; p < 0.001) and KS pigs (258.0 ± 77.8 pg/ml; p < 0.001) at 4 h post injection (F(treatment*time point)12,182.8 = 4.65; p < 0.001). SL pigs showed more sternal recumbancy (296 counts) and were less active (52) than SS pigs (lying inactive sternal: 149 | active: 186, student’s t-test for both models respectively: p < 0.001), KL pigs (174 | 217, p < 0.001), and KS pigs (151 | 215, p < 0.001) during 6 h post injection (lying inactive sternal F(treatment)3,48 = 9.26; p < 0.001; active F(treatment)3,48 = 14.66; p < 0.001). Treatment effects on social behaviour were not consistent. SL pigs performed longer ear exploration (sum per day: 1702.0 s) than SS pigs (465.9 s) two days after i.v. injection, (student’s t-test: p = 0.02; F(treatment*day)6,88 = 1.49, p = 0.19). They did not differ at baseline. The duration of tail exploration (F(treatment*day)6, 87.58 = 0.23, p = 0.97) and manipulation of other body parts (F(treatment*day)6, 88 = 0.36, p = 0.90) did not differ between treatments. LPS activated the HPA-axis (measured 4 h after injection) and elicited so-called sickness behaviour within 6 h after injection as indicated by lower activity in LPS injected pigs.

KW - Tail biting

KW - Risk factors

KW - Pig production

KW - Poor health

KW - Lipopolysaccharide

KW - Inflammation

KW - NSAID

KW - Social Behaviour

KW - Salivary cortisol

KW - HPA-axis

KW - Tail lesions

KW - Pig welfare

KW - Pain

KW - Brain

KW - Neuroinflammation

UR - http://www.isae2019.com/

M3 - Abstract

ER -

Veit C, Janczak A, Ranheim B, Valros A, Sandercock DA, Nordgreen J. The effect of LPS and ketoprofen on social behaviour and brain physiology in group housed pigs. 2019. Abstract from 53rd Congress of the International Society for Applied Ethology (ISAE), Bergen, Norway.