The genomic architecture of resistance to Campylobacter jejuni intestinal colonisation in chickens

A Psifidi, M Fife, J Howell, O Matika, PM van Diemen, R Kuo, J Smith, PM Hocking, N Salmon, MA Jones, DA Hume, G Banos, MP Stevens, P Kaiser

Research output: Contribution to journalArticle

13 Citations (Scopus)
3 Downloads (Pure)

Abstract

Background: Campylobacter is the leading cause of foodborne diarrhoeal illness in humans and is mostly acquired from consumption or handling of contaminated poultry meat. In the absence of effective licensed vaccines and inhibitors, selection for chickens with increased resistance to Campylobacter could potentially reduce its subsequent entry into the food chain. Campylobacter intestinal colonisation levels are influenced by the host genetics of the chicken. In the present study, two chicken populations were used to investigate the genetic architecture of avian resistance to colonisation: (i) a back-cross of two White Leghorn derived inbred lines [(61 x N) x N] known to differ in resistance to Campylobacter colonisation and (ii) a 9th generation advanced intercross (61 x N) line. Results: The level of colonisation with Campylobacter jejuni following experimental infection was found to be a quantitative trait. A back-cross experiment using 1,243 fully informative single nucleotide polymorphism (SNP) markers revealed quantitative trait loci (QTL) on chromosomes 7, 11 and 14. In the advanced intercross line study, the location of the QTL on chromosome 14 was confirmed and refined and two new QTLs were identified located on chromosomes 4 and 16. Pathway and re-sequencing data analysis of the genes located in the QTL candidate regions identified potential pathways, networks and candidate resistance genes. Finally, gene expression analyses were performed for some of the candidate resistance genes to support the results. Conclusion: Campylobacter resistance in chickens is a complex trait, possibly involving the Major Histocompatibility Complex, innate and adaptive immune responses, cadherins and other factors. Two of the QTLs for Campylobacter resistance are co-located with Salmonella resistance loci, indicating that it may be possible to breed simultaneously for enhanced resistance to both zoonoses.
Original languageEnglish
Pages (from-to)293 - 310
Number of pages18
JournalBMC Genomics
Volume17
Early online date18 Apr 2016
DOIs
Publication statusFirst published - 18 Apr 2016

Fingerprint

Campylobacter jejuni
Campylobacter
quantitative trait loci
chickens
genomics
chromosomes
genes
cadherins
poultry meat
White Leghorn
zoonoses
food chain
inbred lines
single nucleotide polymorphism
diarrhea
data analysis
Salmonella
vaccines
breeds
gene expression

Bibliographical note

1028723

Keywords

  • Advanced intercross
  • Back-cross
  • Campylobacter
  • Chicken
  • Genome-wide association
  • Quantitative trait
  • Resistance

Cite this

Psifidi, A., Fife, M., Howell, J., Matika, O., van Diemen, PM., Kuo, R., ... Kaiser, P. (2016). The genomic architecture of resistance to Campylobacter jejuni intestinal colonisation in chickens. BMC Genomics, 17, 293 - 310. https://doi.org/10.1186/s12864-016-2612-7
Psifidi, A ; Fife, M ; Howell, J ; Matika, O ; van Diemen, PM ; Kuo, R ; Smith, J ; Hocking, PM ; Salmon, N ; Jones, MA ; Hume, DA ; Banos, G ; Stevens, MP ; Kaiser, P. / The genomic architecture of resistance to Campylobacter jejuni intestinal colonisation in chickens. In: BMC Genomics. 2016 ; Vol. 17. pp. 293 - 310.
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Psifidi, A, Fife, M, Howell, J, Matika, O, van Diemen, PM, Kuo, R, Smith, J, Hocking, PM, Salmon, N, Jones, MA, Hume, DA, Banos, G, Stevens, MP & Kaiser, P 2016, 'The genomic architecture of resistance to Campylobacter jejuni intestinal colonisation in chickens', BMC Genomics, vol. 17, pp. 293 - 310. https://doi.org/10.1186/s12864-016-2612-7

The genomic architecture of resistance to Campylobacter jejuni intestinal colonisation in chickens. / Psifidi, A; Fife, M; Howell, J; Matika, O; van Diemen, PM; Kuo, R; Smith, J; Hocking, PM; Salmon, N; Jones, MA; Hume, DA; Banos, G; Stevens, MP; Kaiser, P.

In: BMC Genomics, Vol. 17, 18.04.2016, p. 293 - 310.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The genomic architecture of resistance to Campylobacter jejuni intestinal colonisation in chickens

AU - Psifidi, A

AU - Fife, M

AU - Howell, J

AU - Matika, O

AU - van Diemen, PM

AU - Kuo, R

AU - Smith, J

AU - Hocking, PM

AU - Salmon, N

AU - Jones, MA

AU - Hume, DA

AU - Banos, G

AU - Stevens, MP

AU - Kaiser, P

N1 - 1028723

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N2 - Background: Campylobacter is the leading cause of foodborne diarrhoeal illness in humans and is mostly acquired from consumption or handling of contaminated poultry meat. In the absence of effective licensed vaccines and inhibitors, selection for chickens with increased resistance to Campylobacter could potentially reduce its subsequent entry into the food chain. Campylobacter intestinal colonisation levels are influenced by the host genetics of the chicken. In the present study, two chicken populations were used to investigate the genetic architecture of avian resistance to colonisation: (i) a back-cross of two White Leghorn derived inbred lines [(61 x N) x N] known to differ in resistance to Campylobacter colonisation and (ii) a 9th generation advanced intercross (61 x N) line. Results: The level of colonisation with Campylobacter jejuni following experimental infection was found to be a quantitative trait. A back-cross experiment using 1,243 fully informative single nucleotide polymorphism (SNP) markers revealed quantitative trait loci (QTL) on chromosomes 7, 11 and 14. In the advanced intercross line study, the location of the QTL on chromosome 14 was confirmed and refined and two new QTLs were identified located on chromosomes 4 and 16. Pathway and re-sequencing data analysis of the genes located in the QTL candidate regions identified potential pathways, networks and candidate resistance genes. Finally, gene expression analyses were performed for some of the candidate resistance genes to support the results. Conclusion: Campylobacter resistance in chickens is a complex trait, possibly involving the Major Histocompatibility Complex, innate and adaptive immune responses, cadherins and other factors. Two of the QTLs for Campylobacter resistance are co-located with Salmonella resistance loci, indicating that it may be possible to breed simultaneously for enhanced resistance to both zoonoses.

AB - Background: Campylobacter is the leading cause of foodborne diarrhoeal illness in humans and is mostly acquired from consumption or handling of contaminated poultry meat. In the absence of effective licensed vaccines and inhibitors, selection for chickens with increased resistance to Campylobacter could potentially reduce its subsequent entry into the food chain. Campylobacter intestinal colonisation levels are influenced by the host genetics of the chicken. In the present study, two chicken populations were used to investigate the genetic architecture of avian resistance to colonisation: (i) a back-cross of two White Leghorn derived inbred lines [(61 x N) x N] known to differ in resistance to Campylobacter colonisation and (ii) a 9th generation advanced intercross (61 x N) line. Results: The level of colonisation with Campylobacter jejuni following experimental infection was found to be a quantitative trait. A back-cross experiment using 1,243 fully informative single nucleotide polymorphism (SNP) markers revealed quantitative trait loci (QTL) on chromosomes 7, 11 and 14. In the advanced intercross line study, the location of the QTL on chromosome 14 was confirmed and refined and two new QTLs were identified located on chromosomes 4 and 16. Pathway and re-sequencing data analysis of the genes located in the QTL candidate regions identified potential pathways, networks and candidate resistance genes. Finally, gene expression analyses were performed for some of the candidate resistance genes to support the results. Conclusion: Campylobacter resistance in chickens is a complex trait, possibly involving the Major Histocompatibility Complex, innate and adaptive immune responses, cadherins and other factors. Two of the QTLs for Campylobacter resistance are co-located with Salmonella resistance loci, indicating that it may be possible to breed simultaneously for enhanced resistance to both zoonoses.

KW - Advanced intercross

KW - Back-cross

KW - Campylobacter

KW - Chicken

KW - Genome-wide association

KW - Quantitative trait

KW - Resistance

U2 - 10.1186/s12864-016-2612-7

DO - 10.1186/s12864-016-2612-7

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VL - 17

SP - 293

EP - 310

JO - BMC Genomics

JF - BMC Genomics

SN - 1471-2164

ER -