The importance of methanogens associated with ciliate protozoa in ruminal methane production in vitro

C J Newbold, B Lassalas, J P Jouany

Research output: Contribution to journalArticleResearchpeer-review

179 Citations (Scopus)

Abstract

The importance of methanogenic bacteria associated with ciliate protozoa was estimated either by removing protozoa from whole rumen fluid (using defaunated rumen fluid to correct for the effects of centrifugation on bacteria) or by isolating the protozoa. Rumen fluid was withdrawn from sheep inoculated with either Polyplastron multivesiculatum, a co-culture of Isotricha prostoma plus Entodinium spp. or a mixed type B fauna of Entodinium, Eudiplodinium and Epidinium spp. Methanogenesis was highest in rumen fluid containing a mixed protozoal population of the following genera: Entodinium, Eudiplodinium and Epidinium, was lower in defaunated rumen fluid and lowest in rumen fluid containing either I. prostoma plus Entodinium or P. multivesiculatum. Methanogenic bacteria associated with rumen ciliates were apparently responsible for between 9 and 25% of methanogenesis in rumen fluid.

Original languageEnglish
Pages (from-to)230-4
Number of pages5
JournalLetters in Applied Microbiology
Volume21
Issue number4
Publication statusPrint publication - Oct 1995
Externally publishedYes

Fingerprint

Rumen
Methane
Bacteria
In Vitro Techniques
Coculture Techniques
Centrifugation
Sheep
Population

Keywords

  • Animals
  • Ciliophora/microbiology
  • Euryarchaeota/metabolism
  • Fermentation
  • Hexoses/metabolism
  • Methane/metabolism
  • Rumen/metabolism
  • Sheep

Cite this

@article{650d8b9218784feaa7fd3eff5bf28b3a,
title = "The importance of methanogens associated with ciliate protozoa in ruminal methane production in vitro",
abstract = "The importance of methanogenic bacteria associated with ciliate protozoa was estimated either by removing protozoa from whole rumen fluid (using defaunated rumen fluid to correct for the effects of centrifugation on bacteria) or by isolating the protozoa. Rumen fluid was withdrawn from sheep inoculated with either Polyplastron multivesiculatum, a co-culture of Isotricha prostoma plus Entodinium spp. or a mixed type B fauna of Entodinium, Eudiplodinium and Epidinium spp. Methanogenesis was highest in rumen fluid containing a mixed protozoal population of the following genera: Entodinium, Eudiplodinium and Epidinium, was lower in defaunated rumen fluid and lowest in rumen fluid containing either I. prostoma plus Entodinium or P. multivesiculatum. Methanogenic bacteria associated with rumen ciliates were apparently responsible for between 9 and 25{\%} of methanogenesis in rumen fluid.",
keywords = "Animals, Ciliophora/microbiology, Euryarchaeota/metabolism, Fermentation, Hexoses/metabolism, Methane/metabolism, Rumen/metabolism, Sheep",
author = "Newbold, {C J} and B Lassalas and Jouany, {J P}",
year = "1995",
month = "10",
language = "English",
volume = "21",
pages = "230--4",
journal = "Letters in Applied Microbiology",
issn = "0266-8254",
publisher = "Wiley Blackwell",
number = "4",

}

The importance of methanogens associated with ciliate protozoa in ruminal methane production in vitro. / Newbold, C J; Lassalas, B; Jouany, J P.

In: Letters in Applied Microbiology, Vol. 21, No. 4, 10.1995, p. 230-4.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - The importance of methanogens associated with ciliate protozoa in ruminal methane production in vitro

AU - Newbold, C J

AU - Lassalas, B

AU - Jouany, J P

PY - 1995/10

Y1 - 1995/10

N2 - The importance of methanogenic bacteria associated with ciliate protozoa was estimated either by removing protozoa from whole rumen fluid (using defaunated rumen fluid to correct for the effects of centrifugation on bacteria) or by isolating the protozoa. Rumen fluid was withdrawn from sheep inoculated with either Polyplastron multivesiculatum, a co-culture of Isotricha prostoma plus Entodinium spp. or a mixed type B fauna of Entodinium, Eudiplodinium and Epidinium spp. Methanogenesis was highest in rumen fluid containing a mixed protozoal population of the following genera: Entodinium, Eudiplodinium and Epidinium, was lower in defaunated rumen fluid and lowest in rumen fluid containing either I. prostoma plus Entodinium or P. multivesiculatum. Methanogenic bacteria associated with rumen ciliates were apparently responsible for between 9 and 25% of methanogenesis in rumen fluid.

AB - The importance of methanogenic bacteria associated with ciliate protozoa was estimated either by removing protozoa from whole rumen fluid (using defaunated rumen fluid to correct for the effects of centrifugation on bacteria) or by isolating the protozoa. Rumen fluid was withdrawn from sheep inoculated with either Polyplastron multivesiculatum, a co-culture of Isotricha prostoma plus Entodinium spp. or a mixed type B fauna of Entodinium, Eudiplodinium and Epidinium spp. Methanogenesis was highest in rumen fluid containing a mixed protozoal population of the following genera: Entodinium, Eudiplodinium and Epidinium, was lower in defaunated rumen fluid and lowest in rumen fluid containing either I. prostoma plus Entodinium or P. multivesiculatum. Methanogenic bacteria associated with rumen ciliates were apparently responsible for between 9 and 25% of methanogenesis in rumen fluid.

KW - Animals

KW - Ciliophora/microbiology

KW - Euryarchaeota/metabolism

KW - Fermentation

KW - Hexoses/metabolism

KW - Methane/metabolism

KW - Rumen/metabolism

KW - Sheep

M3 - Article

VL - 21

SP - 230

EP - 234

JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

SN - 0266-8254

IS - 4

ER -