The importance of methanogens associated with ciliate protozoa in ruminal methane production in vitro

C J Newbold, B Lassalas, J P Jouany

Research output: Contribution to journalArticlepeer-review

190 Citations (Scopus)

Abstract

The importance of methanogenic bacteria associated with ciliate protozoa was estimated either by removing protozoa from whole rumen fluid (using defaunated rumen fluid to correct for the effects of centrifugation on bacteria) or by isolating the protozoa. Rumen fluid was withdrawn from sheep inoculated with either Polyplastron multivesiculatum, a co-culture of Isotricha prostoma plus Entodinium spp. or a mixed type B fauna of Entodinium, Eudiplodinium and Epidinium spp. Methanogenesis was highest in rumen fluid containing a mixed protozoal population of the following genera: Entodinium, Eudiplodinium and Epidinium, was lower in defaunated rumen fluid and lowest in rumen fluid containing either I. prostoma plus Entodinium or P. multivesiculatum. Methanogenic bacteria associated with rumen ciliates were apparently responsible for between 9 and 25% of methanogenesis in rumen fluid.

Original languageEnglish
Pages (from-to)230-4
Number of pages5
JournalLetters in Applied Microbiology
Volume21
Issue number4
Publication statusPrint publication - Oct 1995
Externally publishedYes

Keywords

  • Animals
  • Ciliophora/microbiology
  • Euryarchaeota/metabolism
  • Fermentation
  • Hexoses/metabolism
  • Methane/metabolism
  • Rumen/metabolism
  • Sheep

Fingerprint Dive into the research topics of 'The importance of methanogens associated with ciliate protozoa in ruminal methane production in vitro'. Together they form a unique fingerprint.

Cite this