The madness of microbiome: attempting to find consensus "best practice" for 16S microbiome studies

J Pollock, L Glendinning, T Wisedchanwet, M Watson

    Research output: Contribution to journalArticlepeer-review

    127 Citations (Scopus)
    25 Downloads (Pure)

    Abstract

    The development and continuous improvement of high-throughput sequencing platforms has stimulated interest in the study of complex microbial communities. Currently, the most popular sequencing approach to study microbial community composition and dynamics is targeted 16S rRNA gene metabarcoding. To prepare samples for sequencing, there are a variety of processing steps, each with the potential to introduce bias at the data analysis stage. In this short review, key information from the literature pertaining to each processing step is described and consequently, general recommendations for future 16S rRNA gene metabarcoding experiments are made.
    Original languageEnglish
    Article numbere02627-17
    JournalApplied and Environmental Microbiology
    Volume84
    Issue number7
    Early online date2 Feb 2018
    DOIs
    Publication statusFirst published - 2 Feb 2018

    Bibliographical note

    1025227

    Keywords

    • 16S RNA
    • DNA sequencing
    • Microbiome

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