TY - JOUR
T1 - The Post-genomic Era of Trichoderma reesei
T2 - What's Next?
AU - Gupta, Vijai Kumar
AU - Steindorff, Andrei Stecca
AU - de Paula, Renato Graciano
AU - Silva-Rocha, Rafael
AU - Mach-Aigner, Astrid R.
AU - Mach, Robert L.
AU - Silva, Roberto N.
PY - 2016/12/1
Y1 - 2016/12/1
N2 - The ascomycete Trichoderma reesei is one of the most well studied cellulolytic microorganisms. This fungus is widely used in the biotechnology industry, mainly in the production of biofuels. Due to its importance, its genome was sequenced in 2008, opening new avenues to study this microorganism. In this ‘post-genomic’ era, a transcriptomic and proteomic era has emerged. Here, we present an overview of new findings in the gene expression regulation network of T. reesei. We also discuss new rational strategies to obtain mutants that produce hydrolytic enzymes with a higher yield, using metabolic engineering. Finally, we present how synthetic biology strategies can be used to create engineered promoters to efficiently synthesize enzymes for biomass degradation to produce bioethanol.
AB - The ascomycete Trichoderma reesei is one of the most well studied cellulolytic microorganisms. This fungus is widely used in the biotechnology industry, mainly in the production of biofuels. Due to its importance, its genome was sequenced in 2008, opening new avenues to study this microorganism. In this ‘post-genomic’ era, a transcriptomic and proteomic era has emerged. Here, we present an overview of new findings in the gene expression regulation network of T. reesei. We also discuss new rational strategies to obtain mutants that produce hydrolytic enzymes with a higher yield, using metabolic engineering. Finally, we present how synthetic biology strategies can be used to create engineered promoters to efficiently synthesize enzymes for biomass degradation to produce bioethanol.
KW - biofuel
KW - cellulases
KW - synthetic biology
KW - transcriptome
KW - Trichoderma reesei
UR - http://www.scopus.com/inward/record.url?scp=84979675926&partnerID=8YFLogxK
U2 - 10.1016/j.tibtech.2016.06.003
DO - 10.1016/j.tibtech.2016.06.003
M3 - Review article
C2 - 27394390
AN - SCOPUS:84979675926
SN - 0167-7799
VL - 34
SP - 970
EP - 982
JO - Trends in Biotechnology
JF - Trends in Biotechnology
IS - 12
ER -