TOPALi: software for automatic identification of recombinant sequences within DNA multiple alignments

Iain Milne, Frank Wright, Glenn Rowe, David F Marshall, Dirk Husmeier, Gràinne McGuire

Research output: Contribution to journalArticlepeer-review

387 Citations (Scopus)


SUMMARY: TOPALi is a new Java graphical analysis application that allows the user to identify recombinant sequences within a DNA multiple alignment (either automatically or via manual investigation). TOPALi allows a choice of three statistical methods to predict the positions of breakpoints due to past recombination. The breakpoint predictions are then used to identify putative recombinant sequences and their relationships to other sequences. In addition to its sophisticated interface, TOPALi can import many sequence formats, estimate and display phylogenetic trees and allow interactive analysis and/or automatic HTML report generation.\n\nAVAILABILITY: TOPALi is freely available from
Original languageEnglish
Pages (from-to)1806-1807
Number of pages2
Issue number11
Publication statusPrint publication - 22 Jul 2004
Externally publishedYes

Cite this