Transmission ratio distortion detection by neutral genetic markers in the Pura Raza Española horse breed

D. I. Perdomo-González*, S. Id-Lahoucine, A. Molina, A. Cánovas, N. Laseca, P. J. Azor, M. Valera

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

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Abstract

Transmission Ratio Distortion (TRD) is a genetic phenomenon widely demonstrated in several livestock species, but barely in equine species. The TRD occurs when certain genotypes are over- or under-represented in the offspring of a particular mating and can be caused by a variety of factors during gamete formation or during embryonic development. For this study, 126 394 trios consisting of a stallion, mare, and offspring were genotyped using a panel of 17 neutral microsatellite markers recommended by the International Society for Animal Genetics for paternity tests and individual identification. The number of alleles available for each marker ranges from 13 to 18, been 268 the total number of alleles investigated. The TRDscan v.2.0 software was used with the biallelic procedure to identify regions with distorted segregation ratios. After completing the analysis, a total of 12 alleles (out of 11 microsatellites) were identified with decisive evidence for genotypic TRD; 3 and 9 with additive and heterosis patterns, respectively. In addition, 19 alleles (out of 10 microsatellites) were identified displaying allelic TRD. Among them, 14 and 5 were parent-unspecific and stallion-mare-specific TRD. Out of the TRD regions, 24 genes were identified and annotated, predominantly associated with cholesterol metabolism and homeostasis. These genes are often linked to non-specific symptoms like impaired fertility, stunted growth, and compromised overall health. The results suggest a significant impact on the inheritance of certain genetic traits in horses. Further analysis and validation are needed to better understand the TRD impact before the potential implementation in the horse breeding programme strategies.

Original languageEnglish
Article number101012
JournalAnimal
Volume17
Issue number11
Early online date10 Nov 2023
DOIs
Publication statusPrint publication - Nov 2023

Bibliographical note

Publisher Copyright:
© 2023 The Author(s)

Keywords

  • Equine
  • Fertility
  • Genotypic expression
  • Mendelian inheritance
  • Microsatellite marker
  • Male
  • Genotype
  • Horses/genetics
  • Genetic Markers
  • Inheritance Patterns
  • Phenotype
  • Animals
  • Alleles
  • Female
  • Software

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