Traumatic neuroma development in tail docked piglets is not associated with long-term changes in spinal nociceptive processing

DA Sandercock*, SH Smith, JE Coe, P Di Giminiani, SA Edwards

*Corresponding author for this work

Research output: Contribution to conferenceAbstract

Abstract

Concerns exist over the long term consequences for tail stump pain experienced by piglets after docking, especially in relation to traumatic neuroma development in caudal nerves after docking injury. Neuroma formation may cause detrimental sensory changes in the tail due to altered axonal excitability leading to abnormal sensation or pain. The aims of this study were to characterize pig tail histopathology at time intervals up to 16 weeks after tail docking and to measure expression of key neuropeptides in caudal dorsal root ganglia and spinal cord neurons associated with (i) peripheral nerve regeneration; activating transcription factor-3 (ATF3), (ii) inflammatory pain; Calcitonin gene-related peptide (CGRP) and (iii) the maintenance of chronic pain; N-methyl D-aspartate (NMDA) ionotropic glutamate receptor subtype 2B (GRIN2B) at the same time points after tail docking injury. Thirty-two female piglets (Landrace/Large White x synthetic sireline) were used (16 docked/16 sham-docked). Piglets were tail docked (amputation of approx. 2/3 of the tail) on post-natal day 3 using a gas hot docking iron. Equivalent sham-docked piglets served as intact controls. Pigs were euthanized by barbiturate overdose 1, 4, 8 and 16 weeks after sham/tail docking. Tail stumps (2 cm) were collected post-mortem for histopathological assessment. Caudal dorsal root ganglia (Ca1-Ca4+) and associated spinal cord were collected for gene expression analysis by real-time quantitative PCR of mRNA. Non-neural inflammatory and reparative epidermal and dermal changes associated with healing were observed after tail docking. Mild inflammation, ulceration and oedema were present at 1 week. Traumatic neuroma development was a consistent feature from 4 weeks after tail docking. Neuroma axonal dispersion in the tail stump was on-going 16 weeks after tail docking. ATF-3 mRNA was significantly upregulated in caudal DRGs up to 8 weeks after tail docking, but did not differ at 16 weeks compared with sham controls. Both CGRP and GRIN2B mRNA expression was significantly upregulated 1 week after tail docking in caudal spinal cord neurons but were not significantly different from sham-docked pigs thereafter. Histopathological lesions that occur shortly after tail docking (beyond 1 week) are not likely to induce or maintain pain. The effects of tail docking on peripheral nerve axonal proliferation and dispersion are relatively short-lived and, although still present, are attenuated by 16 weeks after tail docking injury. Changes in peripheral and spinal nociceptive processing associated with possible inflammatory and chronic pain appear to resolve by 4 weeks after tail docking injury.
Original languageEnglish
Pages611
Number of pages1
Publication statusFirst published - Jun 2016
Event24th International Pig Veterinary Society Congress and 8th European Symposium of Porcine Health Management - Dublin, Ireland
Duration: 1 Jun 2016 → …

Conference

Conference24th International Pig Veterinary Society Congress and 8th European Symposium of Porcine Health Management
CountryIreland
CityDublin
Period1/06/16 → …

Fingerprint

Neuroma
Tail
Pain
Spinal Cord
Swine
Calcitonin Gene-Related Peptide
Wounds and Injuries
Spinal Ganglia
Peripheral Nerves
Chronic Pain
Messenger RNA
Activating Transcription Factor 3
Neurons

Bibliographical note

1026454

Keywords

  • Tail amputation
  • Tail docking
  • Traumatic neuroma
  • Painful procedures
  • Piglets
  • Piglet welfare
  • Pain assessment
  • Neuropathic pain
  • Inflammatory pain
  • Spinal cord
  • Dorsal root ganglia
  • Peripheral nerve injury

Cite this

Sandercock, DA., Smith, SH., Coe, JE., Di Giminiani, P., & Edwards, SA. (2016). Traumatic neuroma development in tail docked piglets is not associated with long-term changes in spinal nociceptive processing. 611. Abstract from 24th International Pig Veterinary Society Congress and 8th European Symposium of Porcine Health Management, Dublin, Ireland.
Sandercock, DA ; Smith, SH ; Coe, JE ; Di Giminiani, P ; Edwards, SA. / Traumatic neuroma development in tail docked piglets is not associated with long-term changes in spinal nociceptive processing. Abstract from 24th International Pig Veterinary Society Congress and 8th European Symposium of Porcine Health Management, Dublin, Ireland.1 p.
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title = "Traumatic neuroma development in tail docked piglets is not associated with long-term changes in spinal nociceptive processing",
abstract = "Concerns exist over the long term consequences for tail stump pain experienced by piglets after docking, especially in relation to traumatic neuroma development in caudal nerves after docking injury. Neuroma formation may cause detrimental sensory changes in the tail due to altered axonal excitability leading to abnormal sensation or pain. The aims of this study were to characterize pig tail histopathology at time intervals up to 16 weeks after tail docking and to measure expression of key neuropeptides in caudal dorsal root ganglia and spinal cord neurons associated with (i) peripheral nerve regeneration; activating transcription factor-3 (ATF3), (ii) inflammatory pain; Calcitonin gene-related peptide (CGRP) and (iii) the maintenance of chronic pain; N-methyl D-aspartate (NMDA) ionotropic glutamate receptor subtype 2B (GRIN2B) at the same time points after tail docking injury. Thirty-two female piglets (Landrace/Large White x synthetic sireline) were used (16 docked/16 sham-docked). Piglets were tail docked (amputation of approx. 2/3 of the tail) on post-natal day 3 using a gas hot docking iron. Equivalent sham-docked piglets served as intact controls. Pigs were euthanized by barbiturate overdose 1, 4, 8 and 16 weeks after sham/tail docking. Tail stumps (2 cm) were collected post-mortem for histopathological assessment. Caudal dorsal root ganglia (Ca1-Ca4+) and associated spinal cord were collected for gene expression analysis by real-time quantitative PCR of mRNA. Non-neural inflammatory and reparative epidermal and dermal changes associated with healing were observed after tail docking. Mild inflammation, ulceration and oedema were present at 1 week. Traumatic neuroma development was a consistent feature from 4 weeks after tail docking. Neuroma axonal dispersion in the tail stump was on-going 16 weeks after tail docking. ATF-3 mRNA was significantly upregulated in caudal DRGs up to 8 weeks after tail docking, but did not differ at 16 weeks compared with sham controls. Both CGRP and GRIN2B mRNA expression was significantly upregulated 1 week after tail docking in caudal spinal cord neurons but were not significantly different from sham-docked pigs thereafter. Histopathological lesions that occur shortly after tail docking (beyond 1 week) are not likely to induce or maintain pain. The effects of tail docking on peripheral nerve axonal proliferation and dispersion are relatively short-lived and, although still present, are attenuated by 16 weeks after tail docking injury. Changes in peripheral and spinal nociceptive processing associated with possible inflammatory and chronic pain appear to resolve by 4 weeks after tail docking injury.",
keywords = "Tail amputation, Tail docking, Traumatic neuroma, Painful procedures, Piglets, Piglet welfare, Pain assessment, Neuropathic pain, Inflammatory pain, Spinal cord, Dorsal root ganglia, Peripheral nerve injury",
author = "DA Sandercock and SH Smith and JE Coe and {Di Giminiani}, P and SA Edwards",
note = "1026454; 24th International Pig Veterinary Society Congress and 8th European Symposium of Porcine Health Management ; Conference date: 01-06-2016",
year = "2016",
month = "6",
language = "English",
pages = "611",

}

Sandercock, DA, Smith, SH, Coe, JE, Di Giminiani, P & Edwards, SA 2016, 'Traumatic neuroma development in tail docked piglets is not associated with long-term changes in spinal nociceptive processing', 24th International Pig Veterinary Society Congress and 8th European Symposium of Porcine Health Management, Dublin, Ireland, 1/06/16 pp. 611.

Traumatic neuroma development in tail docked piglets is not associated with long-term changes in spinal nociceptive processing. / Sandercock, DA; Smith, SH; Coe, JE; Di Giminiani, P; Edwards, SA.

2016. 611 Abstract from 24th International Pig Veterinary Society Congress and 8th European Symposium of Porcine Health Management, Dublin, Ireland.

Research output: Contribution to conferenceAbstract

TY - CONF

T1 - Traumatic neuroma development in tail docked piglets is not associated with long-term changes in spinal nociceptive processing

AU - Sandercock, DA

AU - Smith, SH

AU - Coe, JE

AU - Di Giminiani, P

AU - Edwards, SA

N1 - 1026454

PY - 2016/6

Y1 - 2016/6

N2 - Concerns exist over the long term consequences for tail stump pain experienced by piglets after docking, especially in relation to traumatic neuroma development in caudal nerves after docking injury. Neuroma formation may cause detrimental sensory changes in the tail due to altered axonal excitability leading to abnormal sensation or pain. The aims of this study were to characterize pig tail histopathology at time intervals up to 16 weeks after tail docking and to measure expression of key neuropeptides in caudal dorsal root ganglia and spinal cord neurons associated with (i) peripheral nerve regeneration; activating transcription factor-3 (ATF3), (ii) inflammatory pain; Calcitonin gene-related peptide (CGRP) and (iii) the maintenance of chronic pain; N-methyl D-aspartate (NMDA) ionotropic glutamate receptor subtype 2B (GRIN2B) at the same time points after tail docking injury. Thirty-two female piglets (Landrace/Large White x synthetic sireline) were used (16 docked/16 sham-docked). Piglets were tail docked (amputation of approx. 2/3 of the tail) on post-natal day 3 using a gas hot docking iron. Equivalent sham-docked piglets served as intact controls. Pigs were euthanized by barbiturate overdose 1, 4, 8 and 16 weeks after sham/tail docking. Tail stumps (2 cm) were collected post-mortem for histopathological assessment. Caudal dorsal root ganglia (Ca1-Ca4+) and associated spinal cord were collected for gene expression analysis by real-time quantitative PCR of mRNA. Non-neural inflammatory and reparative epidermal and dermal changes associated with healing were observed after tail docking. Mild inflammation, ulceration and oedema were present at 1 week. Traumatic neuroma development was a consistent feature from 4 weeks after tail docking. Neuroma axonal dispersion in the tail stump was on-going 16 weeks after tail docking. ATF-3 mRNA was significantly upregulated in caudal DRGs up to 8 weeks after tail docking, but did not differ at 16 weeks compared with sham controls. Both CGRP and GRIN2B mRNA expression was significantly upregulated 1 week after tail docking in caudal spinal cord neurons but were not significantly different from sham-docked pigs thereafter. Histopathological lesions that occur shortly after tail docking (beyond 1 week) are not likely to induce or maintain pain. The effects of tail docking on peripheral nerve axonal proliferation and dispersion are relatively short-lived and, although still present, are attenuated by 16 weeks after tail docking injury. Changes in peripheral and spinal nociceptive processing associated with possible inflammatory and chronic pain appear to resolve by 4 weeks after tail docking injury.

AB - Concerns exist over the long term consequences for tail stump pain experienced by piglets after docking, especially in relation to traumatic neuroma development in caudal nerves after docking injury. Neuroma formation may cause detrimental sensory changes in the tail due to altered axonal excitability leading to abnormal sensation or pain. The aims of this study were to characterize pig tail histopathology at time intervals up to 16 weeks after tail docking and to measure expression of key neuropeptides in caudal dorsal root ganglia and spinal cord neurons associated with (i) peripheral nerve regeneration; activating transcription factor-3 (ATF3), (ii) inflammatory pain; Calcitonin gene-related peptide (CGRP) and (iii) the maintenance of chronic pain; N-methyl D-aspartate (NMDA) ionotropic glutamate receptor subtype 2B (GRIN2B) at the same time points after tail docking injury. Thirty-two female piglets (Landrace/Large White x synthetic sireline) were used (16 docked/16 sham-docked). Piglets were tail docked (amputation of approx. 2/3 of the tail) on post-natal day 3 using a gas hot docking iron. Equivalent sham-docked piglets served as intact controls. Pigs were euthanized by barbiturate overdose 1, 4, 8 and 16 weeks after sham/tail docking. Tail stumps (2 cm) were collected post-mortem for histopathological assessment. Caudal dorsal root ganglia (Ca1-Ca4+) and associated spinal cord were collected for gene expression analysis by real-time quantitative PCR of mRNA. Non-neural inflammatory and reparative epidermal and dermal changes associated with healing were observed after tail docking. Mild inflammation, ulceration and oedema were present at 1 week. Traumatic neuroma development was a consistent feature from 4 weeks after tail docking. Neuroma axonal dispersion in the tail stump was on-going 16 weeks after tail docking. ATF-3 mRNA was significantly upregulated in caudal DRGs up to 8 weeks after tail docking, but did not differ at 16 weeks compared with sham controls. Both CGRP and GRIN2B mRNA expression was significantly upregulated 1 week after tail docking in caudal spinal cord neurons but were not significantly different from sham-docked pigs thereafter. Histopathological lesions that occur shortly after tail docking (beyond 1 week) are not likely to induce or maintain pain. The effects of tail docking on peripheral nerve axonal proliferation and dispersion are relatively short-lived and, although still present, are attenuated by 16 weeks after tail docking injury. Changes in peripheral and spinal nociceptive processing associated with possible inflammatory and chronic pain appear to resolve by 4 weeks after tail docking injury.

KW - Tail amputation

KW - Tail docking

KW - Traumatic neuroma

KW - Painful procedures

KW - Piglets

KW - Piglet welfare

KW - Pain assessment

KW - Neuropathic pain

KW - Inflammatory pain

KW - Spinal cord

KW - Dorsal root ganglia

KW - Peripheral nerve injury

M3 - Abstract

SP - 611

ER -

Sandercock DA, Smith SH, Coe JE, Di Giminiani P, Edwards SA. Traumatic neuroma development in tail docked piglets is not associated with long-term changes in spinal nociceptive processing. 2016. Abstract from 24th International Pig Veterinary Society Congress and 8th European Symposium of Porcine Health Management, Dublin, Ireland.