Use of a mannitol rich ensiled grass press juice (EGPJ) as a sole carbon source for polyhydroxyalkanoates (PHAs) production through high cell density cultivation

Federico Cerrone, Reeta Davis, Shane T. Kenny, Trevor Woods, Anthonia O'Donovan, Vijai Kumar Gupta, Maria Tuohy, Ramesh P. Babu, Padraig O'Kiely, Kevin O'Connor*

*Corresponding author for this work

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

This study demonstrates the use of a mannitol rich ensiled grass press juice (EGPJ) as a renewable carbon substrate for polyhydroxyalkanoates (PHA) production in shaking flask experiments and fed-batch stirred tank reactor cultivations. Fed-batch cultivations of Burkholderia sacchari IPT101 using EGPJ as sole carbon source produced 44.5. g/L CDW containing 33% polyhydroxybutyrate (PHB) in 36. h, while Pseudomonas chlororaphis IMD555 produced a CDW of 37. g/L containing 10% of medium chain length polyhydroxyalkanoates (mcl-PHA) in 34. h. PHB and mcl-PHA extracted from B. sacchari IPT101 and P. chlororaphis IMD555, grown on EGPJ, had a molecular weight of 548. kg/mol and 115.4. kg/mol, respectively. While mcl-PHA can be produced from EGPJ, PHB production is more interesting as there is a 4-fold higher volumetric productivity compared to mcl-PHA.

Original languageEnglish
Pages (from-to)45-52
Number of pages8
JournalBioresource Technology
Volume191
Early online date6 May 2015
DOIs
Publication statusPrint publication - Sep 2015
Externally publishedYes

Keywords

  • Biorefining
  • Ensiled grass press juice
  • Mannitol
  • Polyhydroxyalkanoates
  • Pulse feed

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